| 产品: | NAKED2 抗体 |
| 货号: | AF4647 |
| 描述: | Rabbit polyclonal antibody to NAKED2 |
| 应用: | WB |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Zebrafish, Horse, Dog |
| 分子量: | 50kDa; 50kD(Calculated). |
| 蛋白号: | Q969F2 |
| RRID: | AB_2844589 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF4647, RRID:AB_2844589.
展开/折叠
hNkd2; Naked 2; Naked-2; nkd2; NKD2_HUMAN; Protein naked cuticle homolog 2;
抗原和靶标
- Q969F2 NKD2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGKLQSKHAAAARKRRESPEGDSFVASAYASGRKGAEEAERRARDKQELPNGDPKEGPFREDQCPLQVALPAEKAEGREHPGQLLSADDGERAANREGPRGPGGQRLNIDALQCDVSVEEDDRQEWTFTLYDFDNCGKVTREDMSSLMHTIYEVVDASVNHSSGSSKTLRVKLTVSPEPSSKRKEGPPAGQDREPTRCRMEGELAEEPRVADRRLSAHVRRPSTDPQPCSERGPYCVDENTERRNHYLDLAGIENYTSRFGPGSPPVQAKQEPQGRASHLQARSRSQEPDTHAVHHRRSQVLVEHVVPASEPAARALDTQPRPKGPEKQFLKSPKGSGKPPGVPASSKSGKAFSYYLPAVLPPQAPQDGHHLPQPPPPPYGHKRYRQKGREGHSPLKAPHAQPATVEHEVVRDLPPTPAGEGYAVPVIQRHEHHHHHEHHHHHHHHHFHPS
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q969F2 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| G2 | Myristoylation | Uniprot | |
| S18 | Phosphorylation | Uniprot | |
| S23 | Phosphorylation | Uniprot | |
| S31 | Phosphorylation | Uniprot | |
| K74 | Ubiquitination | Uniprot | |
| S176 | Phosphorylation | Uniprot | |
| S216 | Phosphorylation | Uniprot | |
| S223 | Phosphorylation | Uniprot | |
| T224 | Phosphorylation | Uniprot | |
| S230 | Phosphorylation | Uniprot | |
| Y247 | Phosphorylation | Uniprot | |
| Y256 | Phosphorylation | Uniprot | |
| T257 | Phosphorylation | Uniprot | |
| S264 | Phosphorylation | Uniprot | |
| S286 | Phosphorylation | Uniprot | |
| S299 | Phosphorylation | Uniprot | |
| S333 | Phosphorylation | Uniprot | |
| S394 | Phosphorylation | Uniprot | |
| Y423 | Phosphorylation | Uniprot |
研究背景
Cell autonomous antagonist of the canonical Wnt signaling pathway. May activate a second Wnt signaling pathway that controls planar cell polarity (By similarity). Required for processing of TGFA and for targeting of TGFA to the basolateral membrane of polarized epithelial cells.
Ubiquitinated, leading to rapid proteasomal degradation. Interaction with TGFA interferes with RNF25 binding and protects against ubiquitination mediated by RNF25.
Cell membrane. Cytoplasm. Cytoplasmic vesicle.
Expressed in kidney, lung, pancreas and spleen.
Interacts with DVL1, DVL2, DVL3 and PPP2R3A (By similarity). Interacts with RNF25 and TGFA (via cytoplasmic domain).
The N-terminal domain comprising the first 217 amino acid residues is mostly unstructured.
Belongs to the NKD family.
研究领域
· Environmental Information Processing > Signal transduction > Wnt signaling pathway. (View pathway)
限制条款
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