产品: | Eg5 抗体 |
货号: | AF4745 |
描述: | Rabbit polyclonal antibody to Eg5 |
应用: | WB |
反应: | Human, Mouse, Rat |
预测: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken |
分子量: | 120 kDa; 119kD(Calculated). |
蛋白号: | P52732 |
RRID: | AB_2844603 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF4745, RRID:AB_2844603.
展开/折叠
EG5; HKSP; KIF11; KIF11_HUMAN; Kinesin family member 11; Kinesin like protein 1; Kinesin-like protein 1; Kinesin-like protein KIF11; Kinesin-like spindle protein HKSP; Kinesin-related motor protein Eg5; KNSL1; MCLMR; Thyroid receptor-interacting protein 5; TR-interacting protein 5; TRIP-5; TRIP5;
抗原和靶标
- P52732 KIF11_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MASQPNSSAKKKEEKGKNIQVVVRCRPFNLAERKASAHSIVECDPVRKEVSVRTGGLADKSSRKTYTFDMVFGASTKQIDVYRSVVCPILDEVIMGYNCTIFAYGQTGTGKTFTMEGERSPNEEYTWEEDPLAGIIPRTLHQIFEKLTDNGTEFSVKVSLLEIYNEELFDLLNPSSDVSERLQMFDDPRNKRGVIIKGLEEITVHNKDEVYQILEKGAAKRTTAATLMNAYSSRSHSVFSVTIHMKETTIDGEELVKIGKLNLVDLAGSENIGRSGAVDKRAREAGNINQSLLTLGRVITALVERTPHVPYRESKLTRILQDSLGGRTRTSIIATISPASLNLEETLSTLEYAHRAKNILNKPEVNQKLTKKALIKEYTEEIERLKRDLAAAREKNGVYISEENFRVMSGKLTVQEEQIVELIEKIGAVEEELNRVTELFMDNKNELDQCKSDLQNKTQELETTQKHLQETKLQLVKEEYITSALESTEEKLHDAASKLLNTVEETTKDVSGLHSKLDRKKAVDQHNAEAQDIFGKNLNSLFNNMEELIKDGSSKQKAMLEVHKTLFGNLLSSSVSALDTITTVALGSLTSIPENVSTHVSQIFNMILKEQSLAAESKTVLQELINVLKTDLLSSLEMILSPTVVSILKINSQLKHIFKTSLTVADKIEDQKKELDGFLSILCNNLHELQENTICSLVESQKQCGNLTEDLKTIKQTHSQELCKLMNLWTERFCALEEKCENIQKPLSSVQENIQQKSKDIVNKMTFHSQKFCADSDGFSQELRNFNQEGTKLVEESVKHSDKLNGNLEKISQETEQRCESLNTRTVYFSEQWVSSLNEREQELHNLLEVVSQCCEASSSDITEKSDGRKAAHEKQHNIFLDQMTIDEDKLIAQNLELNETIKIGLTKLNCFLEQDLKLDIPTGTTPQRKSYLYPSTLVRTEPREHLLDQLKRKQPELLMMLNCSENNKEETIPDVDVEEAVLGQYTEEPLSQEPSVDAGVDCSSIGGVPFFQHKKSHGKDKENRGINTLERSKVEETTEHLVTKSRLPLRAQINL
种属预测
score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P52732 作为底物
Site | PTM Type | Enzyme | Source |
---|---|---|---|
K34 | Ubiquitination | Uniprot | |
S36 | Phosphorylation | Uniprot | |
K60 | Ubiquitination | Uniprot | |
S62 | Phosphorylation | Uniprot | |
T114 | Phosphorylation | Uniprot | |
S120 | Phosphorylation | Uniprot | |
Y125 | Phosphorylation | Uniprot | |
T126 | Phosphorylation | Uniprot | |
K146 | Acetylation | Uniprot | |
K197 | Ubiquitination | Uniprot | |
K207 | Ubiquitination | Uniprot | |
Y211 | Phosphorylation | Uniprot | |
K216 | Ubiquitination | Uniprot | |
T223 | Phosphorylation | Uniprot | |
T226 | Phosphorylation | Uniprot | |
Y231 | Phosphorylation | Uniprot | |
S232 | Phosphorylation | Uniprot | |
S233 | Phosphorylation | Uniprot | |
S240 | Phosphorylation | Uniprot | |
K257 | Ubiquitination | Uniprot | |
K260 | Ubiquitination | Uniprot | |
S291 | Phosphorylation | Uniprot | |
T294 | Phosphorylation | Uniprot | |
S323 | Phosphorylation | Uniprot | |
K357 | Ubiquitination | Uniprot | |
K362 | Ubiquitination | Uniprot | |
K372 | Acetylation | Uniprot | |
K376 | Acetylation | Uniprot | |
K376 | Ubiquitination | Uniprot | |
K395 | Ubiquitination | Uniprot | |
Y399 | Phosphorylation | Uniprot | |
S401 | Phosphorylation | Uniprot | |
K444 | Ubiquitination | Uniprot | |
K451 | Ubiquitination | Uniprot | |
K457 | Ubiquitination | Uniprot | |
T458 | Phosphorylation | Uniprot | |
T463 | Phosphorylation | Uniprot | |
T464 | Phosphorylation | Uniprot | |
K466 | Ubiquitination | Uniprot | |
K472 | Ubiquitination | Uniprot | |
K477 | Ubiquitination | Uniprot | |
Y480 | Phosphorylation | Uniprot | |
K491 | Ubiquitination | Uniprot | |
K498 | Ubiquitination | Uniprot | |
T506 | Phosphorylation | Uniprot | |
T507 | Phosphorylation | Uniprot | |
K508 | Ubiquitination | Uniprot | |
K516 | Ubiquitination | Uniprot | |
K521 | Ubiquitination | Uniprot | |
K536 | Ubiquitination | Uniprot | |
S540 | Phosphorylation | Uniprot | |
K550 | Ubiquitination | Uniprot | |
K618 | Ubiquitination | Uniprot | |
T643 | Phosphorylation | Uniprot | |
S646 | Phosphorylation | Uniprot | |
S652 | Phosphorylation | Uniprot | |
K655 | Ubiquitination | Uniprot | |
K659 | Ubiquitination | Uniprot | |
T663 | Phosphorylation | Uniprot | |
K667 | Ubiquitination | Uniprot | |
K712 | Ubiquitination | Uniprot | |
K715 | Ubiquitination | Uniprot | |
K724 | Ubiquitination | Uniprot | |
K739 | Ubiquitination | Uniprot | |
K745 | Ubiquitination | Uniprot | |
K757 | Ubiquitination | Uniprot | |
K764 | Ubiquitination | Uniprot | |
K771 | Ubiquitination | Uniprot | |
R784 | Methylation | Uniprot | |
K792 | Ubiquitination | Uniprot | |
K803 | Ubiquitination | Uniprot | |
K810 | Ubiquitination | Uniprot | |
S852 | Phosphorylation | Uniprot | |
S858 | Phosphorylation | Uniprot | |
K875 | Ubiquitination | Uniprot | |
K890 | Ubiquitination | Uniprot | |
K908 | Ubiquitination | Uniprot | |
K918 | Ubiquitination | Uniprot | |
T923 | Phosphorylation | Uniprot | |
T925 | Phosphorylation | Uniprot | |
T926 | Phosphorylation | P06493 (CDK1) | Uniprot |
K930 | Ubiquitination | Uniprot | |
S931 | Phosphorylation | Uniprot | |
Y932 | Phosphorylation | Uniprot | |
Y934 | Phosphorylation | Uniprot | |
S936 | Phosphorylation | Uniprot | |
K952 | Ubiquitination | Uniprot | |
R1025 | Methylation | Uniprot | |
T1029 | Phosphorylation | Uniprot | |
S1033 | Phosphorylation | Q9HC98 (NEK6) | Uniprot |
K1034 | Ubiquitination | Uniprot | |
K1045 | Ubiquitination | Uniprot |
研究背景
Motor protein required for establishing a bipolar spindle during mitosis. Required in non-mitotic cells for transport of secretory proteins from the Golgi complex to the cell surface.
Phosphorylated exclusively on serine during S phase, but on both serine and Thr-926 during mitosis, so controlling the association of KIF11 with the spindle apparatus (probably during early prophase).
A subset of this protein primarily localized at the spindle pole is phosphorylated by NEK6 during mitosis; phosphorylation is required for mitotic function.
Cytoplasm. Cytoplasm>Cytoskeleton>Spindle pole.
Interacts with the thyroid hormone receptor in the presence of thyroid hormone. Component of a large chromatin remodeling complex, at least composed of MYSM1, PCAF, RBM10 and KIF11/TRIP5. Interacts (via C-terminus) with the kinase NEK6 in both interphase and mitosis.
Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. BimC subfamily.
文献引用
Application: WB Species: Human Sample: NSCLC cells
限制条款
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