| 产品: | BACH1/BRIP1 抗体 |
| 货号: | AF4789 |
| 描述: | Rabbit polyclonal antibody to BACH1/BRIP1 |
| 应用: | WB |
| 反应: | Human, Mouse |
| 预测: | Pig, Bovine, Dog, Chicken |
| 分子量: | 145kDa; 141kD(Calculated). |
| 蛋白号: | Q9BX63 |
| RRID: | AB_2844609 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF4789, RRID:AB_2844609.
展开/折叠
ATP dependent RNA helicase BRIP1; ATP-dependent RNA helicase BRIP1; BACH 1; BRAC 1 Associated C Terminal Helicase 1; BRCA 1 Interacting Protein 1; BRCA1 binding helicase like protein BACH1; BRCA1 interacting protein C terminal helicase 1; BRCA1-associated C-terminal helicase 1; BRCA1-interacting protein 1; BRCA1-interacting protein C-terminal helicase 1; BRCA1/BRCA2 associated helicase 1; BRIP 1; BRIP1; FANCJ; FANCJ_HUMAN; Fanconi anemia group J protein; FLJ90232; MGC126521; MGC126523; OF; Protein FACJ;
抗原和靶标
- Q9BX63 FANCJ_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSSMWSEYTIGGVKIYFPYKAYPSQLAMMNSILRGLNSKQHCLLESPTGSGKSLALLCSALAWQQSLSGKPADEGVSEKAEVQLSCCCACHSKDFTNNDMNQGTSRHFNYPSTPPSERNGTSSTCQDSPEKTTLAAKLSAKKQASIYRDENDDFQVEKKRIRPLETTQQIRKRHCFGTEVHNLDAKVDSGKTVKLNSPLEKINSFSPQKPPGHCSRCCCSTKQGNSQESSNTIKKDHTGKSKIPKIYFGTRTHKQIAQITRELRRTAYSGVPMTILSSRDHTCVHPEVVGNFNRNEKCMELLDGKNGKSCYFYHGVHKISDQHTLQTFQGMCKAWDIEELVSLGKKLKACPYYTARELIQDADIIFCPYNYLLDAQIRESMDLNLKEQVVILDEAHNIEDCARESASYSVTEVQLRFARDELDSMVNNNIRKKDHEPLRAVCCSLINWLEANAEYLVERDYESACKIWSGNEMLLTLHKMGITTATFPILQGHFSAVLQKEEKISPIYGKEEAREVPVISASTQIMLKGLFMVLDYLFRQNSRFADDYKIAIQQTYSWTNQIDISDKNGLLVLPKNKKRSRQKTAVHVLNFWCLNPAVAFSDINGKVQTIVLTSGTLSPMKSFSSELGVTFTIQLEANHIIKNSQVWVGTIGSGPKGRNLCATFQNTETFEFQDEVGALLLSVCQTVSQGILCFLPSYKLLEKLKERWLSTGLWHNLELVKTVIVEPQGGEKTNFDELLQVYYDAIKYKGEKDGALLVAVCRGKVSEGLDFSDDNARAVITIGIPFPNVKDLQVELKRQYNDHHSKLRGLLPGRQWYEIQAYRALNQALGRCIRHRNDWGALILVDDRFRNNPSRYISGLSKWVRQQIQHHSTFESALESLAEFSKKHQKVLNVSIKDRTNIQDNESTLEVTSLKYSTSPYLLEAASHLSPENFVEDEAKICVQELQCPKIITKNSPLPSSIISRKEKNDPVFLEEAGKAEKIVISRSTSPTFNKQTKRVSWSSFNSLGQYFTGKIPKATPELGSSENSASSPPRFKTEKMESKTVLPFTDKCESSNLTVNTSFGSCPQSETIISSLKIDATLTRKNHSEHPLCSEEALDPDIELSLVSEEDKQSTSNRDFETEAEDESIYFTPELYDPEDTDEEKNDLAETDRGNRLANNSDCILAKDLFEIRTIKEVDSAREVKAEDCIDTKLNGILHIEESKIDDIDGNVKTTWINELELGKTHEIEIKNFKPSPSKNKGMFPGFK
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9BX63 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y22 | Phosphorylation | Uniprot | |
| K39 | Ubiquitination | Uniprot | |
| S77 | Phosphorylation | Uniprot | |
| Y110 | Phosphorylation | Uniprot | |
| S112 | Phosphorylation | Uniprot | |
| T113 | Phosphorylation | Uniprot | |
| S116 | Phosphorylation | Uniprot | |
| T124 | Phosphorylation | Uniprot | |
| S128 | Phosphorylation | Uniprot | |
| K137 | Ubiquitination | Uniprot | |
| S139 | Phosphorylation | Uniprot | |
| K142 | Ubiquitination | Uniprot | |
| K158 | Ubiquitination | Uniprot | |
| K186 | Acetylation | Uniprot | |
| K191 | Acetylation | Uniprot | |
| S197 | Phosphorylation | Uniprot | |
| S204 | Phosphorylation | Uniprot | |
| S206 | Phosphorylation | Uniprot | |
| K222 | Ubiquitination | Uniprot | |
| S226 | Phosphorylation | Uniprot | |
| S229 | Phosphorylation | Uniprot | |
| S230 | Phosphorylation | Uniprot | |
| T232 | Phosphorylation | Uniprot | |
| K254 | Ubiquitination | Uniprot | |
| T274 | Phosphorylation | Uniprot | |
| S278 | Phosphorylation | Uniprot | |
| K297 | Ubiquitination | Uniprot | |
| K345 | Ubiquitination | Uniprot | |
| K348 | Ubiquitination | Uniprot | |
| Y461 | Phosphorylation | Uniprot | |
| K503 | Ubiquitination | Uniprot | |
| S505 | Phosphorylation | Uniprot | |
| Y508 | Phosphorylation | Uniprot | |
| K510 | Ubiquitination | Uniprot | |
| T584 | Phosphorylation | Uniprot | |
| K656 | Ubiquitination | Uniprot | |
| Y742 | Phosphorylation | Uniprot | |
| K747 | Ubiquitination | Uniprot | |
| K764 | Ubiquitination | Uniprot | |
| K797 | Ubiquitination | Uniprot | |
| K862 | Ubiquitination | Uniprot | |
| S895 | Phosphorylation | Uniprot | |
| K897 | Ubiquitination | Uniprot | |
| Y916 | Phosphorylation | Uniprot | |
| T918 | Phosphorylation | Uniprot | |
| Y921 | Phosphorylation | Uniprot | |
| S927 | Phosphorylation | Uniprot | |
| S930 | Phosphorylation | Uniprot | |
| T953 | Phosphorylation | Uniprot | |
| K954 | Ubiquitination | Uniprot | |
| S956 | Phosphorylation | Uniprot | |
| K982 | Ubiquitination | Uniprot | |
| S988 | Phosphorylation | Uniprot | |
| S990 | Phosphorylation | Uniprot | |
| S1001 | Phosphorylation | Uniprot | |
| S1004 | Phosphorylation | Uniprot | |
| S1007 | Phosphorylation | Uniprot | |
| T1020 | Phosphorylation | Uniprot | |
| S1031 | Phosphorylation | Uniprot | |
| S1032 | Phosphorylation | Uniprot | |
| T1045 | Phosphorylation | Uniprot | |
| T1050 | Phosphorylation | Uniprot | |
| S1063 | Phosphorylation | Uniprot | |
| T1133 | Phosphorylation | Uniprot | |
| T1142 | Phosphorylation | Uniprot | |
| S1162 | Phosphorylation | Uniprot | |
| K1168 | Acetylation | Uniprot | |
| K1168 | Ubiquitination | Uniprot | |
| K1214 | Ubiquitination | Uniprot | |
| S1237 | Phosphorylation | Uniprot | |
| S1239 | Phosphorylation | Uniprot | |
| K1249 | Acetylation | Uniprot |
研究背景
DNA-dependent ATPase and 5' to 3' DNA helicase required for the maintenance of chromosomal stability. Acts late in the Fanconi anemia pathway, after FANCD2 ubiquitination. Involved in the repair of DNA double-strand breaks by homologous recombination in a manner that depends on its association with BRCA1.
Phosphorylated. Phosphorylation is necessary for interaction with BRCA1, and is cell-cycle regulated.
Acetylation at Lys-1249 facilitates DNA end processing required for repair and checkpoint signaling.
Nucleus. Cytoplasm.
Ubiquitously expressed, with highest levels in testis.
Binds directly to the BRCT domains of BRCA1. Interacts with the CIA complex components CIAO1, CIAO2B and MMS19.
4Fe-4S iron-sulfur-binding is required for helicase activity.
Belongs to the DEAD box helicase family. DEAH subfamily.
研究领域
· Genetic Information Processing > Replication and repair > Homologous recombination.
· Genetic Information Processing > Replication and repair > Fanconi anemia pathway.
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