| 产品: | CARD11 抗体 |
| 货号: | AF4790 |
| 描述: | Rabbit polyclonal antibody to CARD11 |
| 应用: | WB |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Chicken |
| 分子量: | 130kDa; 133kD(Calculated). |
| 蛋白号: | Q9BXL7 |
| RRID: | AB_2844610 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF4790, RRID:AB_2844610.
展开/折叠
0610008L17Rik; 2410011D02Rik; Bcl10 interacting maguk protein 3; BIMP 3; BIMP3; CAR11_HUMAN; CARD 11; CARD containing MAGUK protein 3; Card maguk protein 1; CARD-containing MAGUK protein 1; CARD11; CARD11 protein; Carma 1; CARMA1; Caspase recruitment domain containing protein 11; Caspase recruitment domain family member 11; Caspase recruitment domain-containing protein 11; MGC133069;
抗原和靶标
Detected in adult peripheral blood leukocytes, thymus, spleen and liver. Also found in promyelocytic leukemia HL-60 cells, chronic myelogenous leukemia K-562 cells, Burkitt's lymphoma Raji cells and colorectal adenocarcinoma SW480 cells. Not detected in HeLaS3, MOLT-4, A-549 and G431 cells.
- Q9BXL7 CAR11_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MPGGGPEMDDYMETLKDEEDALWENVECNRHMLSRYINPAKLTPYLRQCKVIDEQDEDEVLNAPMLPSKINRAGRLLDILHTKGQRGYVVFLESLEFYYPELYKLVTGKEPTRRFSTIVVEEGHEGLTHFLMNEVIKLQQQMKAKDLQRCELLARLRQLEDEKKQMTLTRVELLTFQERYYKMKEERDSYNDELVKVKDDNYNLAMRYAQLSEEKNMAVMRSRDLQLEIDQLKHRLNKMEEECKLERNQSLKLKNDIENRPKKEQVLELERENEMLKTKNQELQSIIQAGKRSLPDSDKAILDILEHDRKEALEDRQELVNRIYNLQEEARQAEELRDKYLEEKEDLELKCSTLGKDCEMYKHRMNTVMLQLEEVERERDQAFHSRDEAQTQYSQCLIEKDKYRKQIRELEEKNDEMRIEMVRREACIVNLESKLRRLSKDSNNLDQSLPRNLPVTIISQDFGDASPRTNGQEADDSSTSEESPEDSKYFLPYHPPQRRMNLKGIQLQRAKSPISLKRTSDFQAKGHEEEGTDASPSSCGSLPITNSFTKMQPPRSRSSIMSITAEPPGNDSIVRRYKEDAPHRSTVEEDNDSGGFDALDLDDDSHERYSFGPSSIHSSSSSHQSEGLDAYDLEQVNLMFRKFSLERPFRPSVTSVGHVRGPGPSVQHTTLNGDSLTSQLTLLGGNARGSFVHSVKPGSLAEKAGLREGHQLLLLEGCIRGERQSVPLDTCTKEEAHWTIQRCSGPVTLHYKVNHEGYRKLVKDMEDGLITSGDSFYIRLNLNISSQLDACTMSLKCDDVVHVRDTMYQDRHEWLCARVDPFTDHDLDMGTIPSYSRAQQLLLVKLQRLMHRGSREEVDGTHHTLRALRNTLQPEEALSTSDPRVSPRLSRASFLFGQLLQFVSRSENKYKRMNSNERVRIISGSPLGSLARSSLDATKLLTEKQEELDPESELGKNLSLIPYSLVRAFYCERRRPVLFTPTVLAKTLVQRLLNSGGAMEFTICKSDIVTRDEFLRRQKTETIIYSREKNPNAFECIAPANIEAVAAKNKHCLLEAGIGCTRDLIKSNIYPIVLFIRVCEKNIKRFRKLLPRPETEEEFLRVCRLKEKELEALPCLYATVEPDMWGSVEELLRVVKDKIGEEQRKTIWVDEDQL
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9BXL7 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y11 | Phosphorylation | Uniprot | |
| K69 | Ubiquitination | Uniprot | |
| S116 | Phosphorylation | Q9UQM7 (CAMK2A) | Uniprot |
| T117 | Phosphorylation | Uniprot | |
| Y202 | Phosphorylation | Uniprot | |
| Y208 | Phosphorylation | Uniprot | |
| S433 | Phosphorylation | Uniprot | |
| S448 | Phosphorylation | Uniprot | |
| S459 | Phosphorylation | Uniprot | |
| S466 | Phosphorylation | Uniprot | |
| S487 | Phosphorylation | Uniprot | |
| Y489 | Phosphorylation | Uniprot | |
| Y493 | Phosphorylation | Uniprot | |
| S512 | Phosphorylation | Uniprot | |
| S515 | Phosphorylation | Uniprot | |
| T532 | Phosphorylation | Uniprot | |
| S535 | Phosphorylation | Uniprot | |
| S538 | Phosphorylation | Uniprot | |
| S541 | Phosphorylation | Uniprot | |
| S547 | Phosphorylation | Uniprot | |
| T549 | Phosphorylation | Uniprot | |
| S552 | Phosphorylation | Uniprot | |
| S556 | Phosphorylation | Q92918 (MAP4K1) | Uniprot |
| S558 | Phosphorylation | Q92918 (MAP4K1) | Uniprot |
| S559 | Phosphorylation | Q04759 (PRKCQ) , P05771 (PRKCB) | Uniprot |
| S562 | Phosphorylation | Uniprot | |
| S572 | Phosphorylation | Uniprot | |
| S593 | Phosphorylation | Uniprot | |
| S615 | Phosphorylation | P48729 (CSNK1A1) | Uniprot |
| S644 | Phosphorylation | P05771 (PRKCB) , Q04759 (PRKCQ) | Uniprot |
| S645 | Phosphorylation | Uniprot | |
| S652 | Phosphorylation | Q04759 (PRKCQ) , P05771 (PRKCB) | Uniprot |
| K696 | Ubiquitination | Uniprot | |
| K703 | Ubiquitination | Uniprot | |
| S854 | Phosphorylation | Uniprot | |
| T861 | Phosphorylation | Uniprot | |
| T864 | Phosphorylation | Uniprot | |
| T871 | Phosphorylation | Uniprot | |
| S886 | Phosphorylation | Uniprot | |
| S925 | Phosphorylation | Uniprot | |
| S929 | Phosphorylation | Uniprot | |
| K939 | Ubiquitination | Uniprot | |
| K944 | Ubiquitination | Uniprot | |
| S952 | Phosphorylation | Uniprot | |
| Y970 | Phosphorylation | Uniprot | |
| S995 | Phosphorylation | Uniprot | |
| T1002 | Phosphorylation | Uniprot | |
| K1019 | Ubiquitination | Uniprot | |
| K1029 | Ubiquitination | Uniprot |
研究背景
Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Activates NF-kappa-B via BCL10 and IKK. Stimulates the phosphorylation of BCL10. Also activates the TORC1 signaling pathway.
Phosphorylation at Ser-559, Ser-644 and Ser-652 by PRKCB and PRKCQ leads to a shift from an inactive to an active form that activates the NF-kappa-B signaling.
Cytoplasm. Membrane raft.
Note: Colocalized with DPP4 in membrane rafts.
Detected in adult peripheral blood leukocytes, thymus, spleen and liver. Also found in promyelocytic leukemia HL-60 cells, chronic myelogenous leukemia K-562 cells, Burkitt's lymphoma Raji cells and colorectal adenocarcinoma SW480 cells. Not detected in HeLaS3, MOLT-4, A-549 and G431 cells.
Found in a membrane raft complex, at least composed of BCL10, CARD11, DPP4 and IKBKB. CARD11 and BCL10 bind to each other by CARD-CARD interaction. Interacts (via PDZ domain) with DPP4 (via cytoplasmic tail). Interacts with BCL10; as part of a CBM (CARD11-BCL10-MALT1) complex involved in NF-kappa-B activation.
研究领域
· Environmental Information Processing > Signal transduction > NF-kappa B signaling pathway. (View pathway)
· Organismal Systems > Immune system > T cell receptor signaling pathway. (View pathway)
· Organismal Systems > Immune system > B cell receptor signaling pathway. (View pathway)
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