(4)
(2)
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF4605, RRID:AB_2844646.
展开/折叠
BLCPMG; FLJ08163; FLJ18079; FLJ77961; FLJ94056; MGC34277; Occludin; Ocln; OCLN_HUMAN; Tight junction protein occludin;
抗原和靶标
Localized at tight junctions of both epithelial and endothelial cells. Highly expressed in kidney. Not detected in testis.
- Q16625 OCLN_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSSRPLESPPPYRPDEFKPNHYAPSNDIYGGEMHVRPMLSQPAYSFYPEDEILHFYKWTSPPGVIRILSMLIIVMCIAIFACVASTLAWDRGYGTSLLGGSVGYPYGGSGFGSYGSGYGYGYGYGYGYGGYTDPRAAKGFMLAMAAFCFIAALVIFVTSVIRSEMSRTRRYYLSVIIVSAILGIMVFIATIVYIMGVNPTAQSSGSLYGSQIYALCNQFYTPAATGLYVDQYLYHYCVVDPQEAIAIVLGFMIIVAFALIIFFAVKTRRKMDRYDKSNILWDKEHIYDEQPPNVEEWVKNVSAGTQDVPSPPSDYVERVDSPMAYSSNGKVNDKRFYPESSYKSTPVPEVVQELPLTSPVDDFRQPRYSSGGNFETPSKRAPAKGRAGRSKRTEQDHYETDYTTGGESCDELEEDWIREYPPITSDQQRQLYKRNFDTGLQEYKSLQSELDEINKELSRLDKELDDYREESEEYMAAADEYNRLKQVKGSADYKSKKNHCKQLKSKLSHIKKMVGDYDRQKT
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q16625 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S8 | Phosphorylation | Uniprot | |
| K18 | Ubiquitination | Uniprot | |
| S40 | Phosphorylation | Uniprot | |
| Y44 | Phosphorylation | Uniprot | |
| S45 | Phosphorylation | Uniprot | |
| K276 | Ubiquitination | Uniprot | |
| S277 | Phosphorylation | Uniprot | |
| K283 | Ubiquitination | Uniprot | |
| Y287 | Phosphorylation | Uniprot | |
| K299 | Ubiquitination | Uniprot | |
| S302 | Phosphorylation | Uniprot | |
| T305 | Phosphorylation | Uniprot | |
| S310 | Phosphorylation | Uniprot | |
| S313 | Phosphorylation | Uniprot | |
| Y315 | Phosphorylation | Uniprot | |
| S321 | Phosphorylation | Uniprot | |
| Y325 | Phosphorylation | Uniprot | |
| S326 | Phosphorylation | Uniprot | |
| S327 | Phosphorylation | Uniprot | |
| K330 | Ubiquitination | Uniprot | |
| Y337 | Phosphorylation | Uniprot | |
| S340 | Phosphorylation | Uniprot | |
| S341 | Phosphorylation | Uniprot | |
| Y342 | Phosphorylation | Uniprot | |
| K343 | Ubiquitination | Uniprot | |
| S344 | Phosphorylation | Uniprot | |
| T345 | Phosphorylation | Uniprot | |
| S358 | Phosphorylation | Uniprot | |
| Y368 | Phosphorylation | Uniprot | |
| S369 | Phosphorylation | Uniprot | |
| S370 | Phosphorylation | Uniprot | |
| T376 | Phosphorylation | Uniprot | |
| S378 | Phosphorylation | Uniprot | |
| K379 | Ubiquitination | Uniprot | |
| T393 | Phosphorylation | Uniprot | |
| Y398 | Phosphorylation | P12931 (SRC) | Uniprot |
| T400 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| Y402 | Phosphorylation | P12931 (SRC) | Uniprot |
| T403 | Phosphorylation | Q02156 (PRKCE) , P24723 (PRKCH) , Q05513 (PRKCZ) | Uniprot |
| T404 | Phosphorylation | Q02156 (PRKCE) , Q05513 (PRKCZ) , P24723 (PRKCH) , P68400 (CSNK2A1) , P67870 (CSNK2B) | Uniprot |
| S408 | Phosphorylation | P67870 (CSNK2B) , P68400 (CSNK2A1) | Uniprot |
| T424 | Phosphorylation | Q02156 (PRKCE) , Q05513 (PRKCZ) | Uniprot |
| T438 | Phosphorylation | Q02156 (PRKCE) , Q05513 (PRKCZ) | Uniprot |
| Y443 | Phosphorylation | Uniprot | |
| S445 | Phosphorylation | Uniprot | |
| S448 | Phosphorylation | Uniprot | |
| S458 | Phosphorylation | Uniprot | |
| Y467 | Phosphorylation | Uniprot | |
| S471 | Phosphorylation | Uniprot | |
| Y474 | Phosphorylation | Uniprot | |
| Y481 | Phosphorylation | Uniprot | |
| K488 | Ubiquitination | Uniprot | |
| S490 | Phosphorylation | P05771 (PRKCB) | Uniprot |
研究背景
May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions.
Dephosphorylated by PTPRJ. The tyrosine phosphorylation on Tyr-398 and Tyr-402 reduces its ability to interact with TJP1. Phosphorylation at Ser-490 also attenuates the interaction with TJP1.
Cell membrane>Multi-pass membrane protein. Cell junction>Tight junction.
Localized at tight junctions of both epithelial and endothelial cells. Highly expressed in kidney. Not detected in testis.
Interacts with TJP1/ZO1. Interacts with VAPA. Interacts with CLDN1, CLDN6, CLDN9, CLDN11, CLDN12 and CLDN17.
The C-terminal is cytoplasmic and is important for interaction with ZO-1. Sufficient for the tight junction localization. Involved in the regulation of the permeability barrier function of the tight junction (By similarity). The first extracellular loop participates in an adhesive interaction.
Belongs to the ELL/occludin family.
研究领域
· Cellular Processes > Cellular community - eukaryotes > Tight junction. (View pathway)
· Environmental Information Processing > Signaling molecules and interaction > Cell adhesion molecules (CAMs). (View pathway)
· Human Diseases > Infectious diseases: Bacterial > Pathogenic Escherichia coli infection.
· Human Diseases > Infectious diseases: Viral > Hepatitis C.
· Organismal Systems > Immune system > Leukocyte transendothelial migration. (View pathway)
文献引用
Application: WB Species: rat Sample: brain
Application: WB Species: mouse Sample: brain
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