| 产品: | Occludin 抗体 |
| 货号: | AF4606 |
| 描述: | Rabbit polyclonal antibody to Occludin |
| 应用: | WB |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Bovine, Horse, Sheep, Rabbit, Dog |
| 分子量: | 23~30kD, 52~65kD; 59kD(Calculated). |
| 蛋白号: | Q16625 |
| RRID: | AB_2844647 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF4606, RRID:AB_2844647.
展开/折叠
BLCPMG; FLJ08163; FLJ18079; FLJ77961; FLJ94056; MGC34277; Occludin; Ocln; OCLN_HUMAN; Tight junction protein occludin;
抗原和靶标
Localized at tight junctions of both epithelial and endothelial cells. Highly expressed in kidney. Not detected in testis.
- Q16625 OCLN_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSSRPLESPPPYRPDEFKPNHYAPSNDIYGGEMHVRPMLSQPAYSFYPEDEILHFYKWTSPPGVIRILSMLIIVMCIAIFACVASTLAWDRGYGTSLLGGSVGYPYGGSGFGSYGSGYGYGYGYGYGYGGYTDPRAAKGFMLAMAAFCFIAALVIFVTSVIRSEMSRTRRYYLSVIIVSAILGIMVFIATIVYIMGVNPTAQSSGSLYGSQIYALCNQFYTPAATGLYVDQYLYHYCVVDPQEAIAIVLGFMIIVAFALIIFFAVKTRRKMDRYDKSNILWDKEHIYDEQPPNVEEWVKNVSAGTQDVPSPPSDYVERVDSPMAYSSNGKVNDKRFYPESSYKSTPVPEVVQELPLTSPVDDFRQPRYSSGGNFETPSKRAPAKGRAGRSKRTEQDHYETDYTTGGESCDELEEDWIREYPPITSDQQRQLYKRNFDTGLQEYKSLQSELDEINKELSRLDKELDDYREESEEYMAAADEYNRLKQVKGSADYKSKKNHCKQLKSKLSHIKKMVGDYDRQKT
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q16625 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S8 | Phosphorylation | Uniprot | |
| K18 | Ubiquitination | Uniprot | |
| S40 | Phosphorylation | Uniprot | |
| Y44 | Phosphorylation | Uniprot | |
| S45 | Phosphorylation | Uniprot | |
| K276 | Ubiquitination | Uniprot | |
| S277 | Phosphorylation | Uniprot | |
| K283 | Ubiquitination | Uniprot | |
| Y287 | Phosphorylation | Uniprot | |
| K299 | Ubiquitination | Uniprot | |
| S302 | Phosphorylation | Uniprot | |
| T305 | Phosphorylation | Uniprot | |
| S310 | Phosphorylation | Uniprot | |
| S313 | Phosphorylation | Uniprot | |
| Y315 | Phosphorylation | Uniprot | |
| S321 | Phosphorylation | Uniprot | |
| Y325 | Phosphorylation | Uniprot | |
| S326 | Phosphorylation | Uniprot | |
| S327 | Phosphorylation | Uniprot | |
| K330 | Ubiquitination | Uniprot | |
| Y337 | Phosphorylation | Uniprot | |
| S340 | Phosphorylation | Uniprot | |
| S341 | Phosphorylation | Uniprot | |
| Y342 | Phosphorylation | Uniprot | |
| K343 | Ubiquitination | Uniprot | |
| S344 | Phosphorylation | Uniprot | |
| T345 | Phosphorylation | Uniprot | |
| S358 | Phosphorylation | Uniprot | |
| Y368 | Phosphorylation | Uniprot | |
| S369 | Phosphorylation | Uniprot | |
| S370 | Phosphorylation | Uniprot | |
| T376 | Phosphorylation | Uniprot | |
| S378 | Phosphorylation | Uniprot | |
| K379 | Ubiquitination | Uniprot | |
| T393 | Phosphorylation | Uniprot | |
| Y398 | Phosphorylation | P12931 (SRC) | Uniprot |
| T400 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| Y402 | Phosphorylation | P12931 (SRC) | Uniprot |
| T403 | Phosphorylation | Q02156 (PRKCE) , P24723 (PRKCH) , Q05513 (PRKCZ) | Uniprot |
| T404 | Phosphorylation | Q02156 (PRKCE) , Q05513 (PRKCZ) , P24723 (PRKCH) , P68400 (CSNK2A1) , P67870 (CSNK2B) | Uniprot |
| S408 | Phosphorylation | P67870 (CSNK2B) , P68400 (CSNK2A1) | Uniprot |
| T424 | Phosphorylation | Q02156 (PRKCE) , Q05513 (PRKCZ) | Uniprot |
| T438 | Phosphorylation | Q02156 (PRKCE) , Q05513 (PRKCZ) | Uniprot |
| Y443 | Phosphorylation | Uniprot | |
| S445 | Phosphorylation | Uniprot | |
| S448 | Phosphorylation | Uniprot | |
| S458 | Phosphorylation | Uniprot | |
| Y467 | Phosphorylation | Uniprot | |
| S471 | Phosphorylation | Uniprot | |
| Y474 | Phosphorylation | Uniprot | |
| Y481 | Phosphorylation | Uniprot | |
| K488 | Ubiquitination | Uniprot | |
| S490 | Phosphorylation | P05771 (PRKCB) | Uniprot |
研究背景
May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions.
Dephosphorylated by PTPRJ. The tyrosine phosphorylation on Tyr-398 and Tyr-402 reduces its ability to interact with TJP1. Phosphorylation at Ser-490 also attenuates the interaction with TJP1.
Cell membrane>Multi-pass membrane protein. Cell junction>Tight junction.
Localized at tight junctions of both epithelial and endothelial cells. Highly expressed in kidney. Not detected in testis.
Interacts with TJP1/ZO1. Interacts with VAPA. Interacts with CLDN1, CLDN6, CLDN9, CLDN11, CLDN12 and CLDN17.
The C-terminal is cytoplasmic and is important for interaction with ZO-1. Sufficient for the tight junction localization. Involved in the regulation of the permeability barrier function of the tight junction (By similarity). The first extracellular loop participates in an adhesive interaction.
Belongs to the ELL/occludin family.
研究领域
· Cellular Processes > Cellular community - eukaryotes > Tight junction. (View pathway)
· Environmental Information Processing > Signaling molecules and interaction > Cell adhesion molecules (CAMs). (View pathway)
· Human Diseases > Infectious diseases: Bacterial > Pathogenic Escherichia coli infection.
· Human Diseases > Infectious diseases: Viral > Hepatitis C.
· Organismal Systems > Immune system > Leukocyte transendothelial migration. (View pathway)
限制条款
产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。
产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。
Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。产品仅供科学研究使用。不用于诊断和治疗。
产品未经授权不得转售。
Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.