| 产品: | TSC1 抗体 |
| 货号: | AF4609 |
| 描述: | Rabbit polyclonal antibody to TSC1 |
| 应用: | WB |
| 反应: | Human, Mouse, Rat, Monkey |
| 预测: | Horse, Sheep, Dog, Xenopus |
| 分子量: | 150-170kDa; 130kD(Calculated). |
| 蛋白号: | Q92574 |
| RRID: | AB_2844649 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF4609, RRID:AB_2844649.
展开/折叠
Hamartin; kiaa0243; LAM; TSC; Tsc1; Tsc1 gene; TSC1_HUMAN; Tuberous sclerosis 1; Tuberous sclerosis 1 protein; tumor suppressor;
抗原和靶标
Highly expressed in skeletal muscle, followed by heart, brain, placenta, pancreas, lung, liver and kidney. Also expressed in embryonic kidney cells.
- Q92574 TSC1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAQQANVGELLAMLDSPMLGVRDDVTAVFKENLNSDRGPMLVNTLVDYYLETSSQPALHILTTLQEPHDKHLLDRINEYVGKAATRLSILSLLGHVIRLQPSWKHKLSQAPLLPSLLKCLKMDTDVVVLTTGVLVLITMLPMIPQSGKQHLLDFFDIFGRLSSWCLKKPGHVAEVYLVHLHASVYALFHRLYGMYPCNFVSFLRSHYSMKENLETFEEVVKPMMEHVRIHPELVTGSKDHELDPRRWKRLETHDVVIECAKISLDPTEASYEDGYSVSHQISARFPHRSADVTTSPYADTQNSYGCATSTPYSTSRLMLLNMPGQLPQTLSSPSTRLITEPPQATLWSPSMVCGMTTPPTSPGNVPPDLSHPYSKVFGTTAGGKGTPLGTPATSPPPAPLCHSDDYVHISLPQATVTPPRKEERMDSARPCLHRQHHLLNDRGSEEPPGSKGSVTLSDLPGFLGDLASEEDSIEKDKEEAAISRELSEITTAEAEPVVPRGGFDSPFYRDSLPGSQRKTHSAASSSQGASVNPEPLHSSLDKLGPDTPKQAFTPIDLPCGSADESPAGDRECQTSLETSIFTPSPCKIPPPTRVGFGSGQPPPYDHLFEVALPKTAHHFVIRKTEELLKKAKGNTEEDGVPSTSPMEVLDRLIQQGADAHSKELNKLPLPSKSVDWTHFGGSPPSDEIRTLRDQLLLLHNQLLYERFKRQQHALRNRRLLRKVIKAAALEEHNAAMKDQLKLQEKDIQMWKVSLQKEQARYNQLQEQRDTMVTKLHSQIRQLQHDREEFYNQSQELQTKLEDCRNMIAELRIELKKANNKVCHTELLLSQVSQKLSNSESVQQQMEFLNRQLLVLGEVNELYLEQLQNKHSDTTKEVEMMKAAYRKELEKNRSHVLQQTQRLDTSQKRILELESHLAKKDHLLLEQKKYLEDVKLQARGQLQAAESRYEAQKRITQVFELEILDLYGRLEKDGLLKKLEEEKAEAAEAAEERLDCCNDGCSDSMVGHNEEASGHNGETKTPRPSSARGSSGSRGGGGSSSSSSELSTPEKPPHQRAGPFSSRWETTMGEASASIPTTVGSLPSSKSFLGMKARELFRNKSESQCDEDGMTSSLSESLKTELGKDLGVEAKIPLNLDGPHPSPPTPDSVGQLHIMDYNETHHEHS
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q92574 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y79 | Phosphorylation | Uniprot | |
| S91 | Phosphorylation | Uniprot | |
| S102 | Phosphorylation | Uniprot | |
| S108 | Phosphorylation | Uniprot | |
| S115 | Phosphorylation | Uniprot | |
| S205 | Phosphorylation | Uniprot | |
| K221 | Ubiquitination | Uniprot | |
| K238 | Ubiquitination | Uniprot | |
| T267 | Phosphorylation | Uniprot | |
| S270 | Phosphorylation | Uniprot | |
| Y271 | Phosphorylation | Uniprot | |
| Y275 | Phosphorylation | Uniprot | |
| S282 | Phosphorylation | Uniprot | |
| S295 | Phosphorylation | Uniprot | |
| Y297 | Phosphorylation | Uniprot | |
| S303 | Phosphorylation | Uniprot | |
| Y304 | Phosphorylation | Uniprot | |
| T310 | Phosphorylation | Uniprot | |
| Y312 | Phosphorylation | Uniprot | |
| T357 | Phosphorylation | Uniprot | |
| T360 | Phosphorylation | Uniprot | |
| T390 | Phosphorylation | Uniprot | |
| T393 | Phosphorylation | Uniprot | |
| S394 | Phosphorylation | Uniprot | |
| Y406 | Phosphorylation | Uniprot | |
| T417 | Phosphorylation | P06493 (CDK1) | Uniprot |
| S427 | Phosphorylation | Uniprot | |
| S468 | Phosphorylation | Uniprot | |
| S472 | Phosphorylation | Uniprot | |
| K477 | Ubiquitination | Uniprot | |
| S483 | Phosphorylation | Uniprot | |
| S487 | Phosphorylation | O14920 (IKBKB) | Uniprot |
| T490 | Phosphorylation | Uniprot | |
| S505 | Phosphorylation | Uniprot | |
| Y508 | Phosphorylation | Uniprot | |
| S511 | Phosphorylation | O14920 (IKBKB) | Uniprot |
| S515 | Phosphorylation | Uniprot | |
| S521 | Phosphorylation | Uniprot | |
| T547 | Phosphorylation | Uniprot | |
| S579 | Phosphorylation | Uniprot | |
| S584 | Phosphorylation | P06493 (CDK1) | Uniprot |
| S598 | Phosphorylation | Uniprot | |
| Y604 | Phosphorylation | Uniprot | |
| K662 | Ubiquitination | Uniprot | |
| K672 | Ubiquitination | Uniprot | |
| S673 | Phosphorylation | Uniprot | |
| S682 | Phosphorylation | Uniprot | |
| K725 | Ubiquitination | Uniprot | |
| K737 | Ubiquitination | Uniprot | |
| K751 | Acetylation | Uniprot | |
| K756 | Ubiquitination | Uniprot | |
| K774 | Ubiquitination | Uniprot | |
| S871 | Phosphorylation | Uniprot | |
| T873 | Phosphorylation | Uniprot | |
| T874 | Phosphorylation | Uniprot | |
| K934 | Ubiquitination | Uniprot | |
| T1020 | Phosphorylation | Uniprot | |
| S1024 | Phosphorylation | Uniprot | |
| S1025 | Phosphorylation | Uniprot | |
| S1029 | Phosphorylation | Uniprot | |
| S1030 | Phosphorylation | Uniprot | |
| R1033 | Methylation | Uniprot | |
| S1046 | Phosphorylation | Uniprot | |
| T1047 | Phosphorylation | P06493 (CDK1) | Uniprot |
| S1080 | Phosphorylation | Uniprot | |
| S1100 | Phosphorylation | Uniprot | |
| S1102 | Phosphorylation | Uniprot | |
| S1114 | Phosphorylation | Uniprot | |
| S1116 | Phosphorylation | Uniprot | |
| S1141 | Phosphorylation | Uniprot | |
| T1144 | Phosphorylation | Uniprot |
研究背景
In complex with TSC2, inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling. Seems not to be required for TSC2 GAP activity towards RHEB. Implicated as a tumor suppressor. Involved in microtubule-mediated protein transport, but this seems to be due to unregulated mTOR signaling (By similarity). Acts as a co-chaperone for HSP90AA1 facilitating HSP90AA1 chaperoning of protein clients such as kinases, TSC2 and glucocorticoid receptor NR3C1. Increases ATP binding to HSP90AA1 and inhibits HSP90AA1 ATPase activity. Competes with the activating co-chaperone AHSA1 for binding to HSP90AA1, thereby providing a reciprocal regulatory mechanism for chaperoning of client proteins. Recruits TSC2 to HSP90AA1 and stabilizes TSC2 by preventing the interaction between TSC2 and ubiquitin ligase HERC1.
Phosphorylation at Ser-505 does not affect interaction with TSC2.
Cytoplasm. Membrane>Peripheral membrane protein.
Note: At steady state found in association with membranes.
Highly expressed in skeletal muscle, followed by heart, brain, placenta, pancreas, lung, liver and kidney. Also expressed in embryonic kidney cells.
Probably forms a complex composed of chaperones HSP90 and HSP70, co-chaperones STIP1/HOP, CDC37, PPP5C, PTGES3/p23, TSC1 and client protein TSC2. Forms a complex composed of chaperones HSP90 and HSP70, co-chaperones CDC37, PPP5C, TSC1 and client protein TSC2, CDK4, AKT, RAF1 and NR3C1; this complex does not contain co-chaperones STIP1/HOP and PTGES3/p23. Forms a complex containing HSP90AA1, TSC1 and TSC2; TSC1 is required to recruit TCS2 to the complex. Interacts (via C-terminus) with the closed form of HSP90AA1 (via the middle domain and TPR repeat-binding motif). Interacts with TSC2; the interaction stabilizes TSC2 and prevents TSC2 self-aggregation. Interacts with DOCK7. Interacts with FBXW5. Interacts with TBC1D7. Interacts with WDR45B. Interacts with RPAP3 and URI1.
The C-terminal putative coiled-coil domain is necessary for interaction with TSC2.
研究领域
· Cellular Processes > Transport and catabolism > Autophagy - animal. (View pathway)
· Cellular Processes > Cell growth and death > Cellular senescence. (View pathway)
· Environmental Information Processing > Signal transduction > Phospholipase D signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > mTOR signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > AMPK signaling pathway. (View pathway)
· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.
· Human Diseases > Cancers: Overview > Choline metabolism in cancer. (View pathway)
· Organismal Systems > Aging > Longevity regulating pathway. (View pathway)
· Organismal Systems > Endocrine system > Insulin signaling pathway. (View pathway)
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