| 产品: | SIN3A 抗体 |
| 货号: | AF4612 |
| 描述: | Rabbit polyclonal antibody to SIN3A |
| 应用: | WB |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 145kDa; 145kD(Calculated). |
| 蛋白号: | Q96ST3 |
| RRID: | AB_2844651 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF4612, RRID:AB_2844651.
展开/折叠
AW553200; DKFZP434K2235; FLJ90319; Histone deacetylase complex subunit Sin 3a; Histone deacetylase complex subunit Sin3a; KIAA0700; Kiaa4126; mKIAA4126; Paired amphipathic helix protein Sin 3a; Paired amphipathic helix protein Sin3a; Sin 3a; SIN3 homolog A; SIN3 homolog A transcription regulator (yeast); SIN3 homolog A transcription regulator; SIN3 transcription regulator homolog A; Sin3a; SIN3A protein; SIN3A_HUMAN; Transcriptional co repressor Sin 3A; Transcriptional co repressor Sin3A; Transcriptional corepressor Sin3a; Transcriptional regulator SIN3A;
抗原和靶标
Expressed in the developing brain, with highest levels of expression detected in the ventricular zone of various cortical regions.
- Q96ST3 SIN3A_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MKRRLDDQESPVYAAQQRRIPGSTEAFPHQHRVLAPAPPVYEAVSETMQSATGIQYSVTPSYQVSAMPQSSGSHGPAIAAVHSSHHHPTAVQPHGGQVVQSHAHPAPPVAPVQGQQQFQRLKVEDALSYLDQVKLQFGSQPQVYNDFLDIMKEFKSQSIDTPGVISRVSQLFKGHPDLIMGFNTFLPPGYKIEVQTNDMVNVTTPGQVHQIPTHGIQPQPQPPPQHPSQPSAQSAPAPAQPAPQPPPAKVSKPSQLQAHTPASQQTPPLPPYASPRSPPVQPHTPVTISLGTAPSLQNNQPVEFNHAINYVNKIKNRFQGQPDIYKAFLEILHTYQKEQRNAKEAGGNYTPALTEQEVYAQVARLFKNQEDLLSEFGQFLPDANSSVLLSKTTAEKVDSVRNDHGGTVKKPQLNNKPQRPSQNGCQIRRHPTGTTPPVKKKPKLLNLKDSSMADASKHGGGTESLFFDKVRKALRSAEAYENFLRCLVIFNQEVISRAELVQLVSPFLGKFPELFNWFKNFLGYKESVHLETYPKERATEGIAMEIDYASCKRLGSSYRALPKSYQQPKCTGRTPLCKEVLNDTWVSFPSWSEDSTFVSSKKTQYEEHIYRCEDERFELDVVLETNLATIRVLEAIQKKLSRLSAEEQAKFRLDNTLGGTSEVIHRKALQRIYADKAADIIDGLRKNPSIAVPIVLKRLKMKEEEWREAQRGFNKVWREQNEKYYLKSLDHQGINFKQNDTKVLRSKSLLNEIESIYDERQEQATEENAGVPVGPHLSLAYEDKQILEDAAALIIHHVKRQTGIQKEDKYKIKQIMHHFIPDLLFAQRGDLSDVEEEEEEEMDVDEATGAVKKHNGVGGSPPKSKLLFSNTAAQKLRGMDEVYNLFYVNNNWYIFMRLHQILCLRLLRICSQAERQIEEENREREWEREVLGIKRDKSDSPAIQLRLKEPMDVDVEDYYPAFLDMVRSLLDGNIDSSQYEDSLREMFTIHAYIAFTMDKLIQSIVRQLQHIVSDEICVQVTDLYLAENNNGATGGQLNTQNSRSLLESTYQRKAEQLMSDENCFKLMFIQSQGQVQLTIELLDTEEENSDDPVEAERWSDYVERYMNSDTTSPELREHLAQKPVFLPRNLRRIRKCQRGREQQEKEGKEGNSKKTMENVDSLDKLECRFKLNSYKMVYVIKSEDYMYRRTALLRAHQSHERVSKRLHQRFQAWVDKWTKEHVPREMAAETSKWLMGEGLEGLVPCTTTCDTETLHFVSINKYRVKYGTVFKAP
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q96ST3 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S10 | Phosphorylation | Uniprot | |
| Y13 | Phosphorylation | Uniprot | |
| S23 | Phosphorylation | Uniprot | |
| Y41 | Phosphorylation | Uniprot | |
| K122 | Ubiquitination | Uniprot | |
| K155 | Ubiquitination | Uniprot | |
| S166 | Phosphorylation | Uniprot | |
| S169 | Phosphorylation | Uniprot | |
| T260 | Phosphorylation | Uniprot | |
| T266 | Phosphorylation | Uniprot | |
| Y272 | Phosphorylation | Uniprot | |
| S274 | Phosphorylation | Uniprot | |
| S277 | Phosphorylation | Uniprot | |
| T284 | Phosphorylation | Uniprot | |
| S289 | Phosphorylation | Uniprot | |
| Y310 | Phosphorylation | Uniprot | |
| K337 | Ubiquitination | Uniprot | |
| K343 | Ubiquitination | Uniprot | |
| T350 | Phosphorylation | Uniprot | |
| K391 | Ubiquitination | Uniprot | |
| S421 | Phosphorylation | Uniprot | |
| T432 | Phosphorylation | Uniprot | |
| T434 | Phosphorylation | O14757 (CHEK1) | Uniprot |
| T435 | Phosphorylation | Uniprot | |
| S450 | Phosphorylation | Uniprot | |
| S451 | Phosphorylation | Uniprot | |
| K457 | Ubiquitination | Uniprot | |
| K469 | Acetylation | Uniprot | |
| S505 | Phosphorylation | Uniprot | |
| Y524 | Phosphorylation | Uniprot | |
| T539 | Phosphorylation | Uniprot | |
| C551 | S-Nitrosylation | Uniprot | |
| Y558 | Phosphorylation | Uniprot | |
| K563 | Ubiquitination | Uniprot | |
| Y565 | Phosphorylation | Uniprot | |
| T574 | Phosphorylation | Uniprot | |
| T584 | Phosphorylation | Uniprot | |
| K601 | Acetylation | Uniprot | |
| K601 | Ubiquitination | Uniprot | |
| Y610 | Phosphorylation | Uniprot | |
| S641 | Phosphorylation | Uniprot | |
| K650 | Ubiquitination | Uniprot | |
| K676 | Ubiquitination | Uniprot | |
| K697 | Ubiquitination | Uniprot | |
| K715 | Ubiquitination | Uniprot | |
| K723 | Acetylation | Uniprot | |
| K727 | Acetylation | Uniprot | |
| K727 | Ubiquitination | Uniprot | |
| K737 | Methylation | Uniprot | |
| S746 | Phosphorylation | Uniprot | |
| K747 | Ubiquitination | Uniprot | |
| S748 | Phosphorylation | Uniprot | |
| Y757 | Phosphorylation | Uniprot | |
| T802 | Phosphorylation | Uniprot | |
| S832 | Phosphorylation | Uniprot | |
| S860 | Phosphorylation | Uniprot | |
| K865 | Acetylation | Uniprot | |
| K865 | Ubiquitination | Uniprot | |
| K875 | Acetylation | Uniprot | |
| K875 | Ubiquitination | Uniprot | |
| K934 | Acetylation | Uniprot | |
| K934 | Ubiquitination | Uniprot | |
| K937 | Ubiquitination | Uniprot | |
| S938 | Phosphorylation | Uniprot | |
| S940 | Phosphorylation | Uniprot | |
| Y958 | Phosphorylation | Uniprot | |
| Y959 | Phosphorylation | Uniprot | |
| K1053 | Ubiquitination | Uniprot | |
| S1089 | Phosphorylation | Uniprot | |
| Y1101 | Phosphorylation | Uniprot | |
| Y1105 | Phosphorylation | Uniprot | |
| S1108 | Phosphorylation | Uniprot | |
| T1110 | Phosphorylation | Uniprot | |
| T1111 | Phosphorylation | Uniprot | |
| S1112 | Phosphorylation | Uniprot | |
| K1122 | Ubiquitination | Uniprot | |
| K1154 | Ubiquitination | Uniprot | |
| S1161 | Phosphorylation | Uniprot | |
| K1164 | Ubiquitination | Uniprot | |
| K1170 | Ubiquitination | Uniprot | |
| Y1178 | Phosphorylation | Uniprot | |
| K1219 | Ubiquitination | Uniprot | |
| S1231 | Phosphorylation | Uniprot | |
| K1232 | Ubiquitination | Uniprot | |
| K1271 | Ubiquitination | Uniprot |
研究背景
Acts as a transcriptional repressor. Corepressor for REST. Interacts with MXI1 to repress MYC responsive genes and antagonize MYC oncogenic activities. Also interacts with MXD1-MAX heterodimers to repress transcription by tethering SIN3A to DNA. Acts cooperatively with OGT to repress transcription in parallel with histone deacetylation. Involved in the control of the circadian rhythms. Required for the transcriptional repression of circadian target genes, such as PER1, mediated by the large PER complex through histone deacetylation. Cooperates with FOXK1 to regulate cell cycle progression probably by repressing cell cycle inhibitor genes expression (By similarity). Required for cortical neuron differentiation and callosal axon elongation (By similarity).
SUMO1 sumoylated by TOPORS. Probably desumoylated by SENP2.
Nucleus. Nucleus>Nucleolus.
Note: Recruited to the nucleolus by SAP30L.
Expressed in the developing brain, with highest levels of expression detected in the ventricular zone of various cortical regions.
Interacts with ARID4B, BRMS1L, HCFC1, HDAC1, HDAC2, MXI1, SAP30L, SAP130, SFPQ and TOPORS. Interacts with OGT (via TPRs 1-6); the interaction mediates transcriptional repression in parallel with histone deacetylase. Interacts with BAZ2A, MXD1, MXD3, MXD4, MBD2, DACH1, NCOR1, NR4A2, REST, RLIM, SAP30, SETDB1, SMYD2, and SUDS3. Interacts with PHF12 in a complex composed of HDAC1, PHF12 and SAP30 (By similarity). Interacts with TET1; the interaction recruits SIN3A to gene promoters. The large PER complex involved in the histone deacetylation is composed of at least HDAC1, PER2, SFPQ and SIN3A (By similarity). Interacts with KLF11 (By similarity). Interacts with PPHLN1. Found in a complex with YY1, GON4L and HDAC1 (By similarity). Interacts (via PAH2) with FOXK1 (By similarity). Interacts with FOXK2 (By similarity). Found in a complex composed of at least SINHCAF, SIN3A, HDAC1, SAP30, RBBP4, OGT and TET1. Interacts with SINHCAF (By similarity). Interacts with SPHK2.
研究领域
· Human Diseases > Neurodegenerative diseases > Huntington's disease.
· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.
· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway. (View pathway)
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