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| 产品: | 5 Lipoxygenase 抗体 |
| 货号: | AF4699 |
| 描述: | Rabbit polyclonal antibody to 5 Lipoxygenase |
| 应用: | WB |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Bovine, Sheep, Rabbit, Dog |
| 分子量: | 78kDa; 78kD(Calculated). |
| 蛋白号: | P09917 |
| RRID: | AB_2844704 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF4699, RRID:AB_2844704.
展开/折叠
5 LO; 5 LOX; 5-lipoxygenase; 5-LO; 5-LOX; 5LOX; 5LPG; ALOX 5; Alox5; Arachidonate 5 lipoxygenase; Arachidonate 5-lipoxygenase; arachidonic 5-lipoxygenase alpha-10 isoform; arachidonic 5-lipoxygenase delta-10-13 isoform; arachidonic 5-lipoxygenase delta-13 isoform; arachidonic 5-lipoxygenase delta-p10 isoform; Arachidonic acid 5 lipoxygenase; Leukotriene A4 synthase; LOG 5; LOG5; LOX5_HUMAN; MGC163204;
抗原和靶标
- P09917 LOX5_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MPSYTVTVATGSQWFAGTDDYIYLSLVGSAGCSEKHLLDKPFYNDFERGAVDSYDVTVDEELGEIQLVRIEKRKYWLNDDWYLKYITLKTPHGDYIEFPCYRWITGDVEVVLRDGRAKLARDDQIHILKQHRRKELETRQKQYRWMEWNPGFPLSIDAKCHKDLPRDIQFDSEKGVDFVLNYSKAMENLFINRFMHMFQSSWNDFADFEKIFVKISNTISERVMNHWQEDLMFGYQFLNGCNPVLIRRCTELPEKLPVTTEMVECSLERQLSLEQEVQQGNIFIVDFELLDGIDANKTDPCTLQFLAAPICLLYKNLANKIVPIAIQLNQIPGDENPIFLPSDAKYDWLLAKIWVRSSDFHVHQTITHLLRTHLVSEVFGIAMYRQLPAVHPIFKLLVAHVRFTIAINTKAREQLICECGLFDKANATGGGGHVQMVQRAMKDLTYASLCFPEAIKARGMESKEDIPYYFYRDDGLLVWEAIRTFTAEVVDIYYEGDQVVEEDPELQDFVNDVYVYGMRGRKSSGFPKSVKSREQLSEYLTVVIFTASAQHAAVNFGQYDWCSWIPNAPPTMRAPPPTAKGVVTIEQIVDTLPDRGRSCWHLGAVWALSQFQENELFLGMYPEEHFIEKPVKEAMARFRKNLEAIVSVIAERNKKKQLPYYYLSPDRIPNSVAI
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P09917 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y21 | Phosphorylation | Uniprot | |
| Y23 | Phosphorylation | Uniprot | |
| Y43 | Phosphorylation | Uniprot | |
| Y54 | Phosphorylation | Uniprot | |
| Y75 | Phosphorylation | Uniprot | |
| T87 | Phosphorylation | Uniprot | |
| Y95 | Phosphorylation | Uniprot | |
| Y101 | Phosphorylation | Uniprot | |
| T105 | Phosphorylation | Uniprot | |
| Y182 | Phosphorylation | Uniprot | |
| S183 | Phosphorylation | Uniprot | |
| T250 | Phosphorylation | Uniprot | |
| T259 | Phosphorylation | Uniprot | |
| T260 | Phosphorylation | Uniprot | |
| S266 | Phosphorylation | Uniprot | |
| S272 | Phosphorylation | Q9UQM7 (CAMK2A) , P17612 (PRKACA) , P49137 (MAPKAPK2) | Uniprot |
| T445 | Phosphorylation | Uniprot | |
| Y446 | Phosphorylation | Uniprot | |
| S448 | Phosphorylation | Uniprot | |
| T486 | Phosphorylation | Uniprot | |
| S523 | Phosphorylation | Uniprot | |
| S524 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| K528 | Acetylation | Uniprot | |
| S532 | Phosphorylation | Uniprot | |
| S664 | Phosphorylation | P28482 (MAPK1) , P27361 (MAPK3) | Uniprot |
| S671 | Phosphorylation | Uniprot |
研究背景
Catalyzes the first step in leukotriene biosynthesis, and thereby plays a role in inflammatory processes.
Serine phosphorylation by MAPKAPK2 is stimulated by arachidonic acid. Phosphorylation on Ser-523 by PKA has an inhibitory effect. Phosphorylation on Ser-272 prevents export from the nucleus.
Cytoplasm. Nucleus matrix. Nucleus membrane>Peripheral membrane protein.
Note: Shuttles between cytoplasm and nucleus. Found exclusively in the nucleus, when phosphorylated on Ser-272. Calcium binding promotes translocation from the cytosol and the nuclear matrix to the nuclear envelope and membrane association.
Interacts with ALOX5AP and LTC4S. Interacts with COTL1, the interaction is required for stability and efficient catalytic activity.
Belongs to the lipoxygenase family.
研究领域
· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.
· Metabolism > Lipid metabolism > Arachidonic acid metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
· Organismal Systems > Immune system > Fc epsilon RI signaling pathway. (View pathway)
· Organismal Systems > Nervous system > Serotonergic synapse.
· Organismal Systems > Endocrine system > Ovarian steroidogenesis.
文献引用
Application: WB Species: Human Sample: pulmonary artery smooth muscle cells (PASMCs)
限制条款
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