SREBP1 Antibody | Affinity Biosciences LTD|亲科生物官网

产品:	SREBP1 抗体
货号:	AF4728
描述:	Rabbit polyclonal antibody to SREBP1
应用:	WB IHC
反应:	Human, Mouse, Rat
预测:	Pig, Horse, Sheep, Dog, Chicken
分子量:	122kDa; 122kD(Calculated).
蛋白号:	P36956
RRID:	AB_2811173

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二抗
封闭肽

阻断肽(封闭肽)是特异性结合目标抗体和阻断抗体结合的多肽。这些多肽包含抗体识别的抗原表位。与阻断肽结合的抗体不再与靶蛋白上的表位结合。例如，在免疫印迹(WB)和免疫组化(IHC)中，通过比较被阻断抗体和未阻断抗体的染色，可以看到哪种染色是特异性的。

规格	价格	库存
50ul	RMB¥ 1250	现货
100ul	RMB¥ 2300	现货
200ul	RMB¥ 3000	现货

货期: 当天发货

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实验方案

产品描述

来源:

Rabbit

应用:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:

Human,Mouse,Rat

预测:

Pig(100%), Horse(82%), Sheep(100%), Dog(100%), Chicken(83%)

克隆:

Polyclonal

特异性:

SREBP1 Antibody detects endogenous levels of total SREBP1.

RRID:

AB_2811173
引用格式: Affinity Biosciences Cat# AF4728, RRID:AB_2811173.

偶联:

Unconjugated.

纯化:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

保存:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

别名:

展开/折叠

ADD 1; bHLHd1; Class D basic helix-loop-helix protein 1; D630008H06; Processed sterol regulatory element-binding protein 1; SRBP1_HUMAN; SREBF 1; SREBF1; SREBP 1; SREBP 1c; SREBP-1; SREBP1; Sterol regulatory element binding protein 1; Sterol Regulatory Element Binding Transcription Factor 1 / Protein 1; Sterol regulatory element binding transcription factor 1; Sterol regulatory element-binding transcription factor 1;

抗原和靶标

免疫原:

Uniprot:

P36956(SRBP1_HUMAN) >>Visit HPA database.

基因/基因ID:

SREBF1(6720)

表达:

P36956 SRBP1_HUMAN:

Expressed in a wide variety of tissues, most abundant in liver and adrenal gland. In fetal tissues lung and liver shows highest expression. Isoform SREBP-1C predominates in liver, adrenal gland and ovary, whereas isoform SREBP-1A predominates in hepatoma cell lines. Isoform SREBP-1A and isoform SREBP-1C are found in kidney, brain, white fat, and muscle.

序列:

P36956 SRBP1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MDEPPFSEAALEQALGEPCDLDAALLTDIEDMLQLINNQDSDFPGLFDPPYAGSGAGGTDPASPDTSSPGSLSPPPATLSSSLEAFLSGPQAAPSPLSPPQPAPTPLKMYPSMPAFSPGPGIKEESVPLSILQTPTPQPLPGALLPQSFPAPAPPQFSSTPVLGYPSPPGGFSTGSPPGNTQQPLPGLPLASPPGVPPVSLHTQVQSVVPQQLLTVTAAPTAAPVTTTVTSQIQQVPVLLQPHFIKADSLLLTAMKTDGATVKAAGLSPLVSGTTVQTGPLPTLVSGGTILATVPLVVDAEKLPINRLAAGSKAPASAQSRGEKRTAHNAIEKRYRSSINDKIIELKDLVVGTEAKLNKSAVLRKAIDYIRFLQHSNQKLKQENLSLRTAVHKSKSLKDLVSACGSGGNTDVLMEGVKTEVEDTLTPPPSDAGSPFQSSPLSLGSRGSGSGGSGSDSEPDSPVFEDSKAKPEQRPSLHSRGMLDRSRLALCTLVFLCLSCNPLASLLGARGLPSPSDTTSVYHSPGRNVLGTESRDGPGWAQWLLPPVVWLLNGLLVLVSLVLLFVYGEPVTRPHSGPAVYFWRHRKQADLDLARGDFAQAAQQLWLALRALGRPLPTSHLDLACSLLWNLIRHLLQRLWVGRWLAGRAGGLQQDCALRVDASASARDAALVYHKLHQLHTMGKHTGGHLTATNLALSALNLAECAGDAVSVATLAEIYVAAALRVKTSLPRALHFLTRFFLSSARQACLAQSGSVPPAMQWLCHPVGHRFFVDGDWSVLSTPWESLYSLAGNPVDPLAQVTQLFREHLLERALNCVTQPNPSPGSADGDKEFSDALGYLQLLNSCSDAAGAPAYSFSISSSMATTTGVDPVAKWWASLTAVVIHWLRRDEEAAERLCPLVEHLPRVLQESERPLPRAALHSFKAARALLGCAKAESGPASLTICEKASGYLQDSLATTPASSSIDKAVQLFLCDLLLVVRTSLWRQQQPPAPAPAAQGTSSRPQASALELRGFQRDLSSLRRLAQSFRPAMRRVFLHEATARLMAGASPTRTHQLLDRSLRRRAGPGGKGGAVAELEPRPTRREHAEALLLASCYLPPGFLSAPGQRVGMLAEAARTLEKLGDRRLLHDCQQMLMRLGGGTTVTSS

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species

Results

Score

Pig

100

Sheep

100

Dog

100

Chicken

Horse

Bovine

Xenopus

Zebrafish

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P36956 作为底物

P36956

Site	PTM Type	Enzyme	Source
T59	Phosphorylation		Uniprot
S63	Phosphorylation	Q16539 (MAPK14)	Uniprot
S117	Phosphorylation	P27361 (MAPK3) , P28482 (MAPK1) , P45983 (MAPK8)	Uniprot
K123	Sumoylation		Uniprot
K256	Ubiquitination		Uniprot
T275	Phosphorylation		Uniprot
S312	Phosphorylation		Uniprot
K313	Ubiquitination		Uniprot
R321	Methylation		Uniprot
K324	Acetylation		Uniprot
K324	Ubiquitination		Uniprot
K333	Acetylation		Uniprot
S337	Phosphorylation	P57059 (SIK1)	Uniprot
S338	Phosphorylation	P57059 (SIK1) , P17612 (PRKACA)	Uniprot
K342	Ubiquitination		Uniprot
K347	Ubiquitination		Uniprot
K356	Ubiquitination		Uniprot
K359	Ubiquitination		Uniprot
S360	Phosphorylation		Uniprot
K365	Ubiquitination		Uniprot
K379	Ubiquitination		Uniprot
K381	Ubiquitination		Uniprot
S396	Phosphorylation	P54646 (PRKAA2)	Uniprot
K398	Ubiquitination		Uniprot
S402	Phosphorylation	P57059 (SIK1)	Uniprot
K418	Sumoylation		Uniprot
T424	Phosphorylation		Uniprot
T426	Phosphorylation	P49841 (GSK3B) , Q16539 (MAPK14)	Uniprot
S430	Phosphorylation	P49841 (GSK3B)	Uniprot
S434	Phosphorylation	P49841 (GSK3B)	Uniprot
S439	Phosphorylation	P06493 (CDK1)	Uniprot
S448	Phosphorylation		Uniprot
S450	Phosphorylation		Uniprot
S455	Phosphorylation		Uniprot
S457	Phosphorylation		Uniprot
S467	Phosphorylation	P53350 (PLK1)	Uniprot
K470	Ubiquitination		Uniprot
S486	Phosphorylation		Uniprot
Y567	Phosphorylation		Uniprot
K587	Ubiquitination		Uniprot
K675	Ubiquitination		Uniprot
K727	Ubiquitination		Uniprot
K924	Methylation		Uniprot
K924	Ubiquitination		Uniprot
K934	Ubiquitination		Uniprot
K947	Ubiquitination		Uniprot
S1020	Phosphorylation		Uniprot
S1027	Phosphorylation		Uniprot
S1049	Phosphorylation		Uniprot
S1060	Phosphorylation		Uniprot
K1070	Ubiquitination		Uniprot
K1121	Ubiquitination		Uniprot

研究背景

P36956_SRBP1_HUMAN

功能:

Transcriptional activator required for lipid homeostasis. Regulates transcription of the LDL receptor gene as well as the fatty acid and to a lesser degree the cholesterol synthesis pathway (By similarity). Binds to the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3'). Has dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (5'-ATCACCCCAC-3').

翻译修饰:

At low cholesterol the SCAP/SREBP complex is recruited into COPII vesicles for export from the ER. In the Golgi complex SREBPs are cleaved sequentially by site-1 and site-2 protease. The first cleavage by site-1 protease occurs within the luminal loop, the second cleavage by site-2 protease occurs within the first transmembrane domain and releases the transcription factor from the Golgi membrane. Apoptosis triggers cleavage by the cysteine proteases caspase-3 and caspase-7.

Phosphorylated by AMPK, leading to suppress protein processing and nuclear translocation, and repress target gene expression. Phosphorylation at Ser-402 by SIK1 represses activity possibly by inhibiting DNA-binding (By similarity).

细胞定位:

Endoplasmic reticulum membrane>Multi-pass membrane protein. Golgi apparatus membrane>Multi-pass membrane protein. Cytoplasmic vesicle>COPII-coated vesicle membrane>Multi-pass membrane protein.
Note: Moves from the endoplasmic reticulum to the Golgi in the absence of sterols.

Nucleus.

Nucleus.

Nucleus.

组织特异性:

亚基结构:

Forms a tight complex with SCAP in the ER membrane. Efficient DNA binding of the soluble transcription factor fragment requires dimerization with another bHLH protein. Interacts with LMNA. Interacts with CEBPA, the interaction produces a transcriptional synergy (By similarity).

蛋白家族:

The 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.

Belongs to the SREBP family.

研究领域

· Environmental Information Processing > Signal transduction > AMPK signaling pathway. (View pathway)

· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.

· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).

· Organismal Systems > Endocrine system > Insulin signaling pathway. (View pathway)

文献引用

1). Sirtuin 3‐mediated deacetylation of acyl‐CoA synthetase family member 3 by protocatechuic acid attenuates nonalcoholic fatty liver disease. British Journal of Pharmacology, 2020 (PubMed: 32520409) [IF=7.3]

Application: WB Species: Mouse Sample: livers

Figure 3. PCA promotes fatty acid metabolism through SIRT3 activation. (A-E) Western blotting and real-time PCR analysis of hepatic FASN, SREBP-1c, CPT1, Acox1 and PPARα protein (n=5) and mRNA (n=6) levels in mouse livers. *P< 0.05 vs. the WT ND group, #P< 0.05 vs. the WT HFD group, &P< 0.05 vs. the SIRT3−/− ND group. (F-I) The protein expression of FASN, SREBP-1c, CPT1, Acox1, PPARα and SIRT3 was measured by Western blotting (n=5). The mRNA levels of (J) FASN, SREBP-1c (K) CPT1, Acox1 and PPARα were measured with real-time PCR (n=6). *P< 0.05 vs. the si-Control group, #P< 0.05 vs. the si-Control+PA group, &P< 0.05 vs. the si-Control+PA+PCA group.

2). Patchouli alcohol alleviates metabolic dysfunction-associated steatohepatitis via inhibiting mitochondria-associated endoplasmic reticulum membrane disruption-induced hepatic steatosis and inflammation in rats. International immunopharmacology, 2024 (PubMed: 38971107) [IF=5.6]

3). Targeting at cancer energy metabolism and lipid droplet formation as new treatment strategies for epigallocatechin-3-gallate (EGCG) in colorectal cancer cells. Journal of Functional Foods, 2021 [IF=5.6]

Application: WB Species: Human Sample: HCT116 cells

Fig. 3. Effects of EGCG on the expressions of the key enzymes and transcription factor of fatty acid de novo synthesis in HCT116 cells. (A) The expressions of ACLY, FASN, ACC, SCD1, and SREBP1c at the mRNA level as determined using qRT-PCR. (B-C) Western blot and densitometry analysis. The relative intensities of proteins were calculated after normalization to β-Actin. (D-E) Immunofluorescence staining and quantification of FASN, scale bar: 20 μm. Data represent the means ± SE from three independent experiments (A and C) or from three independent experiments with 2 duplicates (E). Statistical analysis was performed using one-way ANOVA with post hoc Bonferroni’s correction test. Groups containing different lowercase letters (a-e) indicate statistical significance at the level of p < 0.05.

4). Si-Ni-San Reduces Hepatic Lipid Deposition in Rats with Metabolic Associated Fatty Liver Disease by AMPK/SIRT1 Pathway. Drug Design, Development and Therapy, 2023 (PubMed: 37808345) [IF=4.8]

Application: WB Species: Rat Sample: liver tissue

Figure 5 Effects of Si-Ni-San (SNS) on the protein expressions related to lipid metabolism. The protein expressions of FAS, CPT-1, nuclear and cytosolic SREBP-1c and PPARα were determined by Western blot. (A and B) Typical bands of Western blot, (C–F) gray value of Western blot bands. The data are stated as the means ± SD. n = 3 *vs CON group (P < 0.05), #vs MOD group (P < 0.05). Liver was obtained from control group (CON), model group (MOD), low-dose SNS group (SNS-L), high-dose SNS group (SNS-H), and metformin group (MET).

5). The Role of cAMP-PKA Pathway in Lactate-Induced Intramuscular Triglyceride Accumulation and Mitochondria Content Increase in Mice. Frontiers in Physiology, 2021 (PubMed: 34588991) [IF=4.0]

Application: WB Species: Mice Sample: gastrocnemius

Figure 7 The expression levels of lipid metabolism-related proteins after chronic lactate and forskolin injection. (A) Western blot analysis and relative fold protein expression of lipolysis-related proteins. (B) Western blot analysis and relative fold protein expression of lipogenesis-related proteins. CP, chronic PBS treated group; CL, chronic lactate treated group; CF, chronic forskolin treated group; CD, chronic DMSO treated group; and CLF, chronic lactate and forskolin treated group. Relative expression levels were normalized to GAPDH. Three bands are used for statistics. The data are presented as the mean±SD, and significant differences among the groups were analyzed with one-way ANOVA. * p<0.05.

6). Tilianin Protects against Nonalcoholic Fatty Liver Disease in Early Obesity Mice. Biological and Pharmaceutical Bulletin, 2023 (PubMed: 36858570) [IF=2.0]

Application: WB Species: Mouse Sample: Liver tissue

Fig. 3. Tilianin Attenuated Aberrant Lipid Metabolism and Oxidative Stress in HFHC-Fed Mice The levels of (A) LDL-C, (B) HDL-C, and (C) TC in the liver. (D) Representative liver sections stained with Oil Red O. (E–I) The protein expression levels of ACC1, FAS, SREBP-1c and LXRα in the liver were analyzed by Western blotting. (J) ROS concentration in mice detected using DHE probe. (K) 4-HNE expression was analyzed by IHC analysis.

7). Efficacy of Ganshuang granules (肝爽颗粒) on non-alcoholic fatty liver and underlying mechanism: a network pharmacology and experimental verification. Journal of Traditional Chinese Medicine, 2024 (PubMed: 38213247)

8). Intramuscular Mitochondrial Adaptation and Lipid Metabolic Alteration in Rats after Chronic High-intensity Interval Training (HIIT) of Different Training Periods. Research Square, 2022

Application: WB Species: Rat Sample:

FIGURE 3 | The expression levels of lipogenesis-related proteins after HIIT. (A) Western blot analysis of lipogenesis-related proteins. (B) Fold protein expression of SREBP-1C, FAS, and the ratio of P-ACC/ACC. Three bands are used for statistics. The data are presented as the mean±SD, and significant differences between the two groups were analyzed with the independent-samples t-test. *P

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SREBP1 Antibody - #AF4728

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