MFF Antibody - #DF12006

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产品: MFF 抗体
货号: DF12006
描述: Rabbit polyclonal antibody to MFF
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Chicken
分子量: 26-29, 35-38 kDa; 38kD(Calculated).
蛋白号: Q9GZY8
RRID: AB_2844811

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Chicken(92%)
克隆:
Polyclonal
特异性:
MFF Antibody detects endogenous levels of total MFF.
RRID:
AB_2844811
引用格式: Affinity Biosciences Cat# DF12006, RRID:AB_2844811.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

C2orf33; Chromosome 2 open reading frame 33; DKFZp666J168; GL004; Mff; MFF_HUMAN; MGC110913; Mitochondrial fission factor; OTTHUMP00000164235;

抗原和靶标

免疫原:
Uniprot:

Q9GZY8(MFF_HUMAN) >>Visit HPA database.

基因/基因ID:
MFF(56947)
表达:
Q9GZY8 MFF_HUMAN:

Highly expressed in heart, kidney, liver, brain, muscle, and stomach.

序列:
MSKGTSSDTSLGRVSRAAFPSPTAAEMAEISRIQYEMEYTEGISQRMRVPEKLKVAPPNADLEQGFQEGVPNASVIMQVPERIVVAGNNEDVSFSRPADLDLIQSTPFKPLALKTPPRVLTLSERPLDFLDLERPPTTPQNEEIRAVGRLKRERSMSENAVRQNGQLVRNDSLWHRSDSAPRNKISRFQAPISAPEYTVTPSPQQARVCPPHMLPEDGANLSSARGILSLIQSSTRRAYQQILDVLDENRRPVLRGGSAAATSNPHHDNVRYGISNIDTTIEGTSDDLTVVDAASLRRQIIKLNRRLQLLEEENKERAKREMVMYSITVAFWLLNSWLWFRR

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Rabbit
100
Chicken
92
Xenopus
50
Dog
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q9GZY8 作为底物

Site PTM Type Enzyme
S21 Phosphorylation
Y35 Phosphorylation
Y39 Phosphorylation
T40 Phosphorylation
K54 Ubiquitination
S74 Phosphorylation
S93 Phosphorylation
T106 Phosphorylation
K109 Ubiquitination
K114 Ubiquitination
T115 Phosphorylation
T121 Phosphorylation
S123 Phosphorylation
T137 Phosphorylation
T138 Phosphorylation
S155 Phosphorylation Q13131 (PRKAA1)
S157 Phosphorylation
S172 Phosphorylation Q13131 (PRKAA1)
S177 Phosphorylation
S179 Phosphorylation
T200 Phosphorylation
S202 Phosphorylation
S222 Phosphorylation
S223 Phosphorylation
S229 Phosphorylation
S233 Phosphorylation
S258 Phosphorylation
S263 Phosphorylation
K302 Ubiquitination

研究背景

功能:

Plays a role in mitochondrial and peroxisomal fission. Promotes the recruitment and association of the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface. May be involved in regulation of synaptic vesicle membrane dynamics by recruitment of DNM1L to clathrin-containing vesicles.

细胞定位:

Mitochondrion outer membrane>Single-pass type IV membrane protein. Peroxisome. Cytoplasmic vesicle>Secretory vesicle>Synaptic vesicle.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Highly expressed in heart, kidney, liver, brain, muscle, and stomach.

亚基结构:

Homodimer. Interacts with DNM1L (By similarity).

蛋白家族:

Belongs to the Tango11 family.

文献引用

1). Valproic acid regulates MIEF1 through MST2-HIPPO to suppress breast cancer growth. Life sciences, 2022 (PubMed: 36126724) [IF=6.1]
2). Paeoniflorin Ameliorates Skeletal Muscle Atrophy in Chronic Kidney Disease via AMPK/SIRT1/PGC-1α-Mediated Oxidative Stress and Mitochondrial Dysfunction. Frontiers in Pharmacology, 2022 (PubMed: 35370668) [IF=5.6]

Application: WB    Species: rat    Sample: C2C12 cells

FIGURE 8 | PGC-1α might be important in mediating the effects of PF on TNF-α-treated C2C12 cells.(I) Representative western blots using antibodies against p-DRP1 (Ser616), DRP1, FIS1, MFF, MTFP1 and GAPDH.
3). Elucidation of SIRT-1/PGC-1α-associated mitochondrial dysfunction and autophagy in nonalcoholic fatty liver disease. Lipids in Health and Disease, 2021 (PubMed: 33902605) [IF=4.5]

Application: WB    Species: Human    Sample: HepG2 cells

Fig. 5 Effect of intervene in SIRT-1 and combine with OA on p62, Beclin-1, LC3B, SIRT-1, PGC-1a, MFN1, MFF and COX-IV protein expression in HepG2 cells. HepG2 cells were treated with 1.5 mM OA for 48 h, following T6 (2 μM) or CAY (20 μM) 2 h. a The p62, Beclin-1 and LC3B protein expression was detected by Western blotting. b The same method was used to detect the SIRT-1, PGC-1a, MFN1, MFF and COX-IV protein expression. The bands were normalized using GAPDH. The images were quantified with ImageJ. Data are presented as the mean ± SD; *P < 0.05, **P < 0.01 compared with CON group; #P < 0.05, ##P < 0.01 compared with OA group

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