产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF12011, RRID:AB_2844816.
展开/折叠
adrenal gland protein AD-005; CCR4 NOT transcription complex subunit 1 isoform b; CCR4-associated factor 1; hNOT1; Negative regulator of transcription subunit 1 homolog; NOT1 (negative regulator of transcription 1 yeast) homolog; NOT1H;
抗原和靶标
Strongly expressed in brain, heart, thymus, spleen, kidney, liver, placenta and lung. Weakly expressed in skeletal muscle and colon.
- A5YKK6 CNOT1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MNLDSLSLALSQISYLVDNLTKKNYRASQQEIQHIVNRHGPEADRHLLRCLFSHVDFSGDGKSSGKDFHQTQFLIQECALLITKPNFISTLSYAIDNPLHYQKSLKPAPHLFAQLSKVLKLSKVQEVIFGLALLNSSSSDLRGFAAQFIKQKLPDLLRSYIDADVSGNQEGGFQDIAIEVLHLLLSHLLFGQKGAFGVGQEQIDAFLKTLRRDFPQERCPVVLAPLLYPEKRDILMDRILPDSGGVAKTMMESSLADFMQEVGYGFCASIEECRNIIVQFGVREVTAAQVARVLGMMARTHSGLTDGIPLQSISAPGSGIWSDGKDKSDGAQAHTWNVEVLIDVLKELNPSLNFKEVTYELDHPGFQIRDSKGLHNVVYGIQRGLGMEVFPVDLIYRPWKHAEGQLSFIQHSLINPEIFCFADYPCHTVATDILKAPPEDDNREIATWKSLDLIESLLRLAEVGQYEQVKQLFSFPIKHCPDMLVLALLQINTSWHTLRHELISTLMPIFLGNHPNSAIILHYAWHGQGQSPSIRQLIMHAMAEWYMRGEQYDQAKLSRILDVAQDLKALSMLLNGTPFAFVIDLAALASRREYLKLDKWLTDKIREHGEPFIQACMTFLKRRCPSILGGLAPEKDQPKSAQLPPETLATMLACLQACAGSVSQELSETILTMVANCSNVMNKARQPPPGVMPKGRPPSASSLDAISPVQIDPLAGMTSLSIGGSAAPHTQSMQGFPPNLGSAFSTPQSPAKAFPPLSTPNQTTAFSGIGGLSSQLPVGGLGTGSLTGIGTGALGLPAVNNDPFVQRKLGTSGLNQPTFQQSKMKPSDLSQVWPEANQHFSKEIDDEANSYFQRIYNHPPHPTMSVDEVLEMLQRFKDSTIKREREVFNCMLRNLFEEYRFFPQYPDKELHITACLFGGIIEKGLVTYMALGLALRYVLEALRKPFGSKMYYFGIAALDRFKNRLKDYPQYCQHLASISHFMQFPHHLQEYIEYGQQSRDPPVKMQGSITTPGSIALAQAQAQAQVPAKAPLAGQVSTMVTTSTTTTVAKTVTVTRPTGVSFKKDVPPSINTTNIDTLLVATDQTERIVEPPENIQEKIAFIFNNLSQSNMTQKVEELKETVKEEFMPWVSQYLVMKRVSIEPNFHSLYSNFLDTLKNPEFNKMVLNETYRNIKVLLTSDKAAANFSDRSLLKNLGHWLGMITLAKNKPILHTDLDVKSLLLEAYVKGQQELLYVVPFVAKVLESSIRSVVFRPPNPWTMAIMNVLAELHQEHDLKLNLKFEIEVLCKNLALDINELKPGNLLKDKDRLKNLDEQLSAPKKDVKQPEELPPITTTTTSTTPATNTTCTATVPPQPQYSYHDINVYSLAGLAPHITLNPTIPLFQAHPQLKQCVRQAIERAVQELVHPVVDRSIKIAMTTCEQIVRKDFALDSEESRMRIAAHHMMRNLTAGMAMITCREPLLMSISTNLKNSFASALRTASPQQREMMDQAAAQLAQDNCELACCFIQKTAVEKAGPEMDKRLATEFELRKHARQEGRRYCDPVVLTYQAERMPEQIRLKVGGVDPKQLAVYEEFARNVPGFLPTNDLSQPTGFLAQPMKQAWATDDVAQIYDKCITELEQHLHAIPPTLAMNPQAQALRSLLEVVVLSRNSRDAIAALGLLQKAVEGLLDATSGADADLLLRYRECHLLVLKALQDGRAYGSPWCNKQITRCLIECRDEYKYNVEAVELLIRNHLVNMQQYDLHLAQSMENGLNYMAVAFAMQLVKILLVDERSVAHVTEADLFHTIETLMRINAHSRGNAPEGLPQLMEVVRSNYEAMIDRAHGGPNFMMHSGISQASEYDDPPGLREKAEYLLREWVNLYHSAAAGRDSTKAFSAFVGQMHQQGILKTDDLITRFFRLCTEMCVEISYRAQAEQQHNPAANPTMIRAKCYHNLDAFVRLIALLVKHSGEATNTVTKINLLNKVLGIVVGVLLQDHDVRQSEFQQLPYHRIFIMLLLELNAPEHVLETINFQTLTAFCNTFHILRPTKAPGFVYAWLELISHRIFIARMLAHTPQQKGWPMYAQLLIDLFKYLAPFLRNVELTKPMQILYKGTLRVLLVLLHDFPEFLCDYHYGFCDVIPPNCIQLRNLILSAFPRNMRLPDPFTPNLKVDMLSEINIAPRILTNFTGVMPPQFKKDLDSYLKTRSPVTFLSDLRSNLQVSNEPGNRYNLQLINALVLYVGTQAIAHIHNKGSTPSMSTITHSAHMDIFQNLAVDLDTEGRYLFLNAIANQLRYPNSHTHYFSCTMLYLFAEANTEAIQEQITRVLLERLIVNRPHPWGLLITFIELIKNPAFKFWNHEFVHCAPEIEKLFQSVAQCCMGQKQAQQVMEGTGAS
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - A5YKK6 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S7 | Phosphorylation | Uniprot | |
| T21 | Phosphorylation | Uniprot | |
| R45 | Methylation | Uniprot | |
| K231 | Ubiquitination | Uniprot | |
| R238 | Methylation | Uniprot | |
| S318 | Phosphorylation | Uniprot | |
| S322 | Phosphorylation | Uniprot | |
| K355 | Ubiquitination | Uniprot | |
| K372 | Ubiquitination | Uniprot | |
| K435 | Ubiquitination | Uniprot | |
| K449 | Ubiquitination | Uniprot | |
| K556 | Ubiquitination | Uniprot | |
| K599 | Ubiquitination | Uniprot | |
| C624 | S-Nitrosylation | Uniprot | |
| S626 | Phosphorylation | Uniprot | |
| K635 | Ubiquitination | Uniprot | |
| S725 | Phosphorylation | Uniprot | |
| T730 | Phosphorylation | Uniprot | |
| K808 | Ubiquitination | Uniprot | |
| K823 | Ubiquitination | Uniprot | |
| Y851 | Phosphorylation | Uniprot | |
| K877 | Acetylation | Uniprot | |
| K882 | Acetylation | Uniprot | |
| T927 | Phosphorylation | Uniprot | |
| Y928 | Phosphorylation | Uniprot | |
| Y937 | Phosphorylation | Uniprot | |
| S948 | Phosphorylation | Uniprot | |
| T1051 | Phosphorylation | Uniprot | |
| T1053 | Phosphorylation | Uniprot | |
| T1058 | Phosphorylation | Uniprot | |
| S1061 | Phosphorylation | Uniprot | |
| K1063 | Acetylation | Uniprot | |
| K1063 | Ubiquitination | Uniprot | |
| K1064 | Ubiquitination | Uniprot | |
| T1073 | Phosphorylation | Uniprot | |
| K1114 | Ubiquitination | Uniprot | |
| K1163 | Ubiquitination | Uniprot | |
| R1189 | Methylation | Uniprot | |
| K1208 | Ubiquitination | Uniprot | |
| Y1234 | Phosphorylation | Uniprot | |
| K1310 | Ubiquitination | Uniprot | |
| K1320 | Ubiquitination | Uniprot | |
| K1321 | Ubiquitination | Uniprot | |
| T1418 | Phosphorylation | Uniprot | |
| T1419 | Phosphorylation | Uniprot | |
| K1426 | Ubiquitination | Uniprot | |
| S1432 | Phosphorylation | Uniprot | |
| K1514 | Acetylation | Uniprot | |
| K1514 | Ubiquitination | Uniprot | |
| T1525 | Phosphorylation | Uniprot | |
| K1560 | Ubiquitination | Uniprot | |
| K1567 | Ubiquitination | Uniprot | |
| K1600 | Ubiquitination | Uniprot | |
| S1649 | Phosphorylation | Uniprot | |
| S1703 | Phosphorylation | Uniprot | |
| K1708 | Ubiquitination | Uniprot | |
| Y1817 | Phosphorylation | Uniprot | |
| Y1842 | Phosphorylation | Uniprot | |
| K1851 | Ubiquitination | Uniprot | |
| Y2074 | Phosphorylation | Uniprot | |
| K2086 | Ubiquitination | Uniprot | |
| K2151 | Ubiquitination | Uniprot | |
| K2177 | Ubiquitination | Uniprot | |
| K2178 | Ubiquitination | Uniprot | |
| K2185 | Ubiquitination | Uniprot | |
| S2188 | Phosphorylation | Uniprot | |
| S2194 | Phosphorylation | Uniprot | |
| Y2264 | Phosphorylation | Uniprot | |
| K2364 | Ubiquitination | Uniprot |
研究背景
Scaffolding component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. Its scaffolding function implies its interaction with the catalytic complex module and diverse RNA-binding proteins mediating the complex recruitment to selected mRNA 3'UTRs. Involved in degradation of AU-rich element (ARE)-containing mRNAs probably via association with ZFP36. Mediates the recruitment of the CCR4-NOT complex to miRNA targets and to the RISC complex via association with TNRC6A, TNRC6B or TNRC6C. Acts as a transcriptional repressor. Represses the ligand-dependent transcriptional activation by nuclear receptors. Involved in the maintenance of embryonic stem (ES) cell identity.
Cytoplasm>P-body. Nucleus.
Note: NANOS2 promotes its localization to P-body.
Strongly expressed in brain, heart, thymus, spleen, kidney, liver, placenta and lung. Weakly expressed in skeletal muscle and colon.
Component of the CCR4-NOT complex; distinct complexes seem to exist that differ in the participation of probably mutually exclusive catalytic subunits. In the complex, interacts directly with CNOT6, CNOT6L, CNOT7 or CNOT8. Interacts in a ligand-dependent fashion with ESR1 and RXRA. Interacts with NANOS2, TOB1 and ZFP36. Interacts with TNRC6A, TNRC6B or TNRC6C; the interactions are direct. Interacts with YTHDF2; the interaction is direct and promotes recruitment of the CCR4-NOT complex to N6-methyladenosine (m6A)-containing mRNAs, leading to their deadenylation and subsequent degradation.
Contains Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs, a motif known to be important for the association with nuclear receptors.
Belongs to the CNOT1 family.
研究领域
· Genetic Information Processing > Folding, sorting and degradation > RNA degradation.
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