产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF12022, RRID:AB_2844827.
展开/折叠
FAK family kinase-interacting protein of 200 kDa; FIP200; RB1-inducible coiled-coil protein 1; RB1CC1; RBCC1_HUMAN;
抗原和靶标
Expression levels correlated closely with those of RB1 in cancer cell lines as well as in various normal human tissues. Abundantly expressed in human musculoskeletal and cultured osteosarcoma cells.
- Q8TDY2 RBCC1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MKLYVFLVNTGTTLTFDTELTVQTVADLKHAIQSKYKIAIQHQVLVVNGGECMAADRRVCTYSAGTDTNPIFLFNKEMILCDRPPAIPKTTFSTENDMEIKVEESLMMPAVFHTVASRTQLALEMYEVAKKLCSFCEGLVHDEHLQHQGWAAIMANLEDCSNSYQKLLFKFESIYSNYLQSIEDIKLKLTHLGTAVSVMAKIPLLECLTRHSYRECLGRLDSLPEHEDSEKAEMKRSTELVLSPDMPRTTNESLLTSFPKSVEHVSPDTADAESGKEIRESCQSTVHQQDETTIDTKDGDLPFFNVSLLDWINVQDRPNDVESLVRKCFDSMSRLDPRIIRPFIAECRQTIAKLDNQNMKAIKGLEDRLYALDQMIASCGRLVNEQKELAQGFLANQKRAENLKDASVLPDLCLSHANQLMIMLQNHRKLLDIKQKCTTAKQELANNLHVRLKWCCFVMLHADQDGEKLQALLRLVIELLERVKIVEALSTVPQMYCLAVVEVVRRKMFIKHYREWAGALVKDGKRLYEAEKSKRESFGKLFRKSFLRNRLFRGLDSWPPSFCTQKPRKFDCELPDISLKDLQFLQSFCPSEVQPFLRVPLLCDFEPLHQHVLALHNLVKAAQSLDEMSQTITDLLSEQKASVSQTSPQSASSPRMESTAGITTTTSPRTPPPLTVQDPLCPAVCPLEELSPDSIDAHTFDFETIPHPNIEQTIHQVSLDLDSLAESPESDFMSAVNEFVIEENLSSPNPISDPQSPEMMVESLYSSVINAIDSRRMQDTNVCGKEDFGDHTSLNVQLERCRVVAQDSHFSIQTIKEDLCHFRTFVQKEQCDFSNSLKCTAVEIRNIIEKVKCSLEITLKEKHQKELLSLKNEYEGKLDGLIKETEENENKIKKLKGELVCLEEVLQNKDNEFALVKHEKEAVICLQNEKDQKLLEMENIMHSQNCEIKELKQSREIVLEDLKKLHVENDEKLQLLRAELQSLEQSHLKELEDTLQVRHIQEFEKVMTDHRVSLEELKKENQQIINQIQESHAEIIQEKEKQLQELKLKVSDLSDTRCKLEVELALKEAETDEIKILLEESRAQQKETLKSLLEQETENLRTEISKLNQKIQDNNENYQVGLAELRTLMTIEKDQCISELISRHEEESNILKAELNKVTSLHNQAFEIEKNLKEQIIELQSKLDSELSALERQKDEKITQQEEKYEAIIQNLEKDRQKLVSSQEQDREQLIQKLNCEKDEAIQTALKEFKLEREVVEKELLEKVKHLENQIAKSPAIDSTRGDSSSLVAELQEKLQEEKAKFLEQLEEQEKRKNEEMQNVRTSLIAEQQTNFNTVLTREKMRKENIINDLSDKLKSTMQQQERDKDLIESLSEDRARLLEEKKKLEEEVSKLRSSSFVPSPYVATAPELYGACAPELPGESDRSAVETADEGRVDSAMETSMMSVQENIHMLSEEKQRIMLLERTLQLKEEENKRLNQRLMSQSMSSVSSRHSEKIAIRDFQVGDLVLIILDERHDNYVLFTVSPTLYFLHSESLPALDLKPGEGASGASRRPWVLGKVMEKEYCQAKKAQNRFKVPLGTKFYRVKAVSWNKKV
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q8TDY2 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Phosphorylation | Uniprot | ||
| K35 | Ubiquitination | Uniprot | |
| S105 | Phosphorylation | Uniprot | |
| S134 | Phosphorylation | Uniprot | |
| T209 | Phosphorylation | Uniprot | |
| R210 | Methylation | Uniprot | |
| S212 | Phosphorylation | Uniprot | |
| R214 | Methylation | Uniprot | |
| S222 | Phosphorylation | Uniprot | |
| S229 | Phosphorylation | Uniprot | |
| S237 | Phosphorylation | Uniprot | |
| T238 | Phosphorylation | Uniprot | |
| S243 | Phosphorylation | Uniprot | |
| S253 | Phosphorylation | Uniprot | |
| T256 | Phosphorylation | Uniprot | |
| S257 | Phosphorylation | Uniprot | |
| K260 | Ubiquitination | Uniprot | |
| S261 | Phosphorylation | Uniprot | |
| S266 | Phosphorylation | Uniprot | |
| S274 | Phosphorylation | Uniprot | |
| K387 | Ubiquitination | Uniprot | |
| K434 | Ubiquitination | Uniprot | |
| S490 | Phosphorylation | Uniprot | |
| T491 | Phosphorylation | Uniprot | |
| Y496 | Phosphorylation | Uniprot | |
| K522 | Ubiquitination | Uniprot | |
| S537 | Phosphorylation | Uniprot | |
| S624 | Phosphorylation | Uniprot | |
| S629 | Phosphorylation | Uniprot | |
| T633 | Phosphorylation | Uniprot | |
| S642 | Phosphorylation | Uniprot | |
| S644 | Phosphorylation | Uniprot | |
| T646 | Phosphorylation | Uniprot | |
| S647 | Phosphorylation | Uniprot | |
| S650 | Phosphorylation | Uniprot | |
| S652 | Phosphorylation | Uniprot | |
| S653 | Phosphorylation | Uniprot | |
| T664 | Phosphorylation | Uniprot | |
| T670 | Phosphorylation | Uniprot | |
| S793 | Phosphorylation | Uniprot | |
| K828 | Ubiquitination | Uniprot | |
| K865 | Ubiquitination | Uniprot | |
| K871 | Ubiquitination | Uniprot | |
| K877 | Ubiquitination | Uniprot | |
| K883 | Acetylation | Uniprot | |
| K891 | Acetylation | Uniprot | |
| K896 | Ubiquitination | Uniprot | |
| K917 | Ubiquitination | Uniprot | |
| S943 | Phosphorylation | Uniprot | |
| K963 | Sumoylation | Uniprot | |
| K963 | Ubiquitination | Uniprot | |
| S982 | Phosphorylation | Uniprot | |
| S986 | Phosphorylation | Uniprot | |
| K989 | Ubiquitination | Uniprot | |
| T994 | Phosphorylation | Uniprot | |
| S1051 | Phosphorylation | Uniprot | |
| K1090 | Ubiquitination | Uniprot | |
| K1110 | Ubiquitination | Uniprot | |
| S1160 | Phosphorylation | Uniprot | |
| K1204 | Ubiquitination | Uniprot | |
| Y1205 | Phosphorylation | Uniprot | |
| S1222 | Phosphorylation | Uniprot | |
| K1233 | Ubiquitination | Uniprot | |
| K1247 | Ubiquitination | Uniprot | |
| K1263 | Acetylation | Uniprot | |
| S1274 | Phosphorylation | Uniprot | |
| S1323 | Phosphorylation | Uniprot | |
| S1370 | Phosphorylation | Uniprot | |
| T1440 | Phosphorylation | Uniprot | |
| S1441 | Phosphorylation | Uniprot | |
| S1482 | Phosphorylation | Uniprot | |
| S1484 | Phosphorylation | Uniprot | |
| K1581 | Ubiquitination | Uniprot |
研究背景
Involved in autophagy. Regulates early events but also late events of autophagosome formation through direct interaction with Atg16L1. Required for the formation of the autophagosome-like double-membrane structure that surrounds the Salmonella-containing vacuole (SCV) during S.typhimurium infection and subsequent xenophagy (By similarity). Involved in repair of DNA damage caused by ionizing radiation, which subsequently improves cell survival by decreasing apoptosis (By similarity). Inhibits PTK2/FAK1 and PTK2B/PYK2 kinase activity, affecting their downstream signaling pathways. Plays a role as a modulator of TGF-beta-signaling by restricting substrate specificity of RNF111 (By similarity). Functions as a DNA-binding transcription factor. Is a potent regulator of the RB1 pathway through induction of RB1 expression. Plays a crucial role in muscular differentiation. Plays an indispensable role in fetal hematopoiesis and in the regulation of neuronal homeostasis (By similarity).
Nucleus. Cytoplasm. Cytoplasm>Cytosol. Preautophagosomal structure. Lysosome.
Note: Under starvation conditions, is localized to puncate structures primarily representing the isolation membrane that sequesters a portion of the cytoplasm resulting in the formation of an autophagosome.
Expression levels correlated closely with those of RB1 in cancer cell lines as well as in various normal human tissues. Abundantly expressed in human musculoskeletal and cultured osteosarcoma cells.
Part of a complex consisting of ATG13/KIAA0652, ULK1 and RB1CC1. This complex associates with ATG101. Interacts with PTK2/FAK1 and PTK2B/PYK2. Interacts with GABARAP and GABARAPL1. Interacts with ATG16L1; the interaction is required for ULK1 complex-dependent autophagy. Interacts with RNF111, SKI and SMAD7 (By similarity). Interacts with COP1 in the cytoplasm of proliferating cells in response to UV stimulation. Interacts with TP53. Interacts with C9orf72. Interacts with WDR45B.
Belongs to the ATG17 family.
研究领域
· Cellular Processes > Transport and catabolism > Autophagy - animal. (View pathway)
· Organismal Systems > Aging > Longevity regulating pathway. (View pathway)
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