(1)
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产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF12023, RRID:AB_2844828.
展开/折叠
epididymis secretory protein Li 49; HEL-S-49; MGC88108; TIM; TPI 1; TPI; TPI1; TPID; Triose phosphate isomerase 1; Triose phosphate isomerase; Triosephosphate isomerase 1; Triosephosphate isomerase;
抗原和靶标
- P60174 TPIS_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAEDGEEAEFHFAALYISGQWPRLRADTDLQRLGSSAMAPSRKFFVGGNWKMNGRKQSLGELIGTLNAAKVPADTEVVCAPPTAYIDFARQKLDPKIAVAAQNCYKVTNGAFTGEISPGMIKDCGATWVVLGHSERRHVFGESDELIGQKVAHALAEGLGVIACIGEKLDEREAGITEKVVFEQTKVIADNVKDWSKVVLAYEPVWAIGTGKTATPQQAQEVHEKLRGWLKSNVSDAVAQSTRIIYGGSVTGATCKELASQPDVDGFLVGGASLKPEFVDIINAKQ
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P60174 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y16 | Phosphorylation | Uniprot | |
| T28 | Phosphorylation | Uniprot | |
| K43 | Acetylation | Uniprot | |
| K43 | Ubiquitination | Uniprot | |
| K51 | Acetylation | Uniprot | |
| K51 | Methylation | Uniprot | |
| K51 | Ubiquitination | Uniprot | |
| K56 | Ubiquitination | Uniprot | |
| S58 | Phosphorylation | Uniprot | |
| T65 | Phosphorylation | Uniprot | |
| K70 | Ubiquitination | Uniprot | |
| Y85 | Phosphorylation | Uniprot | |
| K92 | Ubiquitination | Uniprot | |
| K96 | Acetylation | Uniprot | |
| K96 | Ubiquitination | Uniprot | |
| Y105 | Phosphorylation | Uniprot | |
| K106 | Acetylation | Uniprot | |
| K106 | Methylation | Uniprot | |
| K106 | Ubiquitination | Uniprot | |
| T108 | Phosphorylation | Uniprot | |
| S117 | Phosphorylation | Uniprot | |
| K122 | Ubiquitination | Uniprot | |
| T127 | Phosphorylation | Uniprot | |
| R137 | Methylation | Uniprot | |
| S143 | Phosphorylation | Uniprot | |
| K168 | Acetylation | Uniprot | |
| K168 | Ubiquitination | Uniprot | |
| R172 | Methylation | Uniprot | |
| T177 | Phosphorylation | Uniprot | |
| K179 | Acetylation | Uniprot | |
| K179 | Sumoylation | Uniprot | |
| K179 | Ubiquitination | Uniprot | |
| K186 | Acetylation | Uniprot | |
| K186 | Ubiquitination | Uniprot | |
| K193 | Acetylation | Uniprot | |
| K193 | Ubiquitination | Uniprot | |
| S196 | Phosphorylation | Uniprot | |
| K197 | Ubiquitination | Uniprot | |
| Y202 | Phosphorylation | Uniprot | |
| T210 | Phosphorylation | Uniprot | |
| K212 | Acetylation | Uniprot | |
| K212 | Ubiquitination | Uniprot | |
| T215 | Phosphorylation | Uniprot | |
| K225 | Acetylation | Uniprot | |
| K225 | Ubiquitination | Uniprot | |
| K231 | Acetylation | Uniprot | |
| K231 | Methylation | Uniprot | |
| K231 | Ubiquitination | Uniprot | |
| S232 | Phosphorylation | Uniprot | |
| S235 | Phosphorylation | Uniprot | |
| S241 | Phosphorylation | Uniprot | |
| Y246 | Phosphorylation | Uniprot | |
| S249 | Phosphorylation | Uniprot | |
| T251 | Phosphorylation | Uniprot | |
| T254 | Phosphorylation | Uniprot | |
| C255 | S-Nitrosylation | Uniprot | |
| K256 | Ubiquitination | Uniprot | |
| S260 | Phosphorylation | Uniprot | |
| K275 | Acetylation | Uniprot | |
| K275 | Ubiquitination | Uniprot | |
| K285 | Acetylation | Uniprot | |
| K285 | Ubiquitination | Uniprot |
研究背景
Triosephosphate isomerase is an extremely efficient metabolic enzyme that catalyzes the interconversion between dihydroxyacetone phosphate (DHAP) and D-glyceraldehyde-3-phosphate (G3P) in glycolysis and gluconeogenesis.
It is also responsible for the non-negligible production of methylglyoxal a reactive cytotoxic side-product that modifies and can alter proteins, DNA and lipids.
The initiator methionine for isoform 2 is removed.
Cytoplasm.
Homodimer.
Belongs to the triosephosphate isomerase family.
研究领域
· Metabolism > Carbohydrate metabolism > Glycolysis / Gluconeogenesis.
· Metabolism > Carbohydrate metabolism > Fructose and mannose metabolism.
· Metabolism > Carbohydrate metabolism > Inositol phosphate metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
· Metabolism > Global and overview maps > Carbon metabolism.
· Metabolism > Global and overview maps > Biosynthesis of amino acids.
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