产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF12025, RRID:AB_2844830.
展开/折叠
C80529; EC 4.2.1.17; ECHS 1; Enoyl CoA hydratase 1; Enoyl CoA hydratase mitochondrial; Enoyl CoA hydratase short chain 1 mitochondrial; Enoyl Coenzyme A hydratase short chain 1; Enoyl Coenzyme A hydratase short chain 1 mitochondrial; Mitochondrial short chain enoyl coenzyme A hydratase 1; OTTHUMP00000020811; SCEH; Short chain enoyl CoA hydratase;
抗原和靶标
- P30084 ECHM_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAALRVLLSCVRGPLRPPVRCPAWRPFASGANFEYIIAEKRGKNNTVGLIQLNRPKALNALCDGLIDELNQALKTFEEDPAVGAIVLTGGDKAFAAGADIKEMQNLSFQDCYSSKFLKHWDHLTQVKKPVIAAVNGYAFGGGCELAMMCDIIYAGEKAQFAQPEILIGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLVSKICPVETLVEEAIQCAEKIASNSKIVVAMAKESVNAAFEMTLTEGSKLEKKLFYSTFATDDRKEGMTAFVEKRKANFKDQ
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P30084 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y35 | Phosphorylation | Uniprot | |
| K40 | Acetylation | Uniprot | |
| T46 | Phosphorylation | Uniprot | |
| K56 | Ubiquitination | Uniprot | |
| C62 | S-Nitrosylation | Uniprot | |
| K92 | Acetylation | Uniprot | |
| K92 | Ubiquitination | Uniprot | |
| S107 | Phosphorylation | Uniprot | |
| S114 | Phosphorylation | Uniprot | |
| K115 | Ubiquitination | Uniprot | |
| K118 | Acetylation | Uniprot | |
| K118 | Ubiquitination | Uniprot | |
| Y137 | Phosphorylation | Uniprot | |
| T176 | Phosphorylation | Uniprot | |
| S186 | Phosphorylation | Uniprot | |
| T194 | Phosphorylation | Uniprot | |
| R197 | Methylation | Uniprot | |
| S199 | Phosphorylation | Uniprot | |
| K204 | Acetylation | Uniprot | |
| K204 | Ubiquitination | Uniprot | |
| K211 | Ubiquitination | Uniprot | |
| C213 | S-Nitrosylation | Uniprot | |
| C225 | S-Nitrosylation | Uniprot | |
| T251 | Phosphorylation | Uniprot | |
| K261 | Ubiquitination | Uniprot |
研究背景
Straight-chain enoyl-CoA thioesters from C4 up to at least C16 are processed, although with decreasing catalytic rate. Has high substrate specificity for crotonyl-CoA and moderate specificity for acryloyl-CoA, 3-methylcrotonyl-CoA and methacrylyl-CoA. It is noteworthy that binds tiglyl-CoA, but hydrates only a small amount of this substrate.
Mitochondrion matrix.
Liver, fibroblast, muscle. Barely detectable in spleen and kidney.
Homohexamer; dimer of trimers.
Belongs to the enoyl-CoA hydratase/isomerase family.
研究领域
· Metabolism > Lipid metabolism > Fatty acid elongation.
· Metabolism > Lipid metabolism > Fatty acid degradation.
· Metabolism > Amino acid metabolism > Valine, leucine and isoleucine degradation.
· Metabolism > Amino acid metabolism > Lysine degradation.
· Metabolism > Amino acid metabolism > Tryptophan metabolism.
· Metabolism > Metabolism of other amino acids > beta-Alanine metabolism.
· Metabolism > Carbohydrate metabolism > Propanoate metabolism.
· Metabolism > Carbohydrate metabolism > Butanoate metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
· Metabolism > Global and overview maps > Carbon metabolism.
· Metabolism > Global and overview maps > Fatty acid metabolism.
限制条款
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