PLOD3 Antibody - #DF12034
| 产品: | PLOD3 抗体 |
| 货号: | DF12034 |
| 描述: | Rabbit polyclonal antibody to PLOD3 |
| 应用: | WB IHC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Horse, Sheep, Rabbit, Dog |
| 分子量: | 80-85 kDa; 85kD(Calculated). |
| 蛋白号: | O60568 |
| RRID: | AB_2844839 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF12034, RRID:AB_2844839.
展开/折叠
2-oxoglutarate 5-dioxygenase 3; bone protein I; BP-I; LH3; Lysine hydroxylase 3; Lysyl hydroxylase 3; Plod3; PLOD3_HUMAN; Procollagen lysine,2 oxoglutarate 5 dioxygenase 3 precursor; Procollagen-lysine;
抗原和靶标
Ubiquitous (PubMed:9724729). Detected in heart, placenta and pancreas and at lower levels in lung, liver and skeletal muscle (PubMed:9582318, PubMed:9724729).
- O60568 PLOD3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MTSSGPGPRFLLLLPLLLPPAASASDRPRGRDPVNPEKLLVITVATAETEGYLRFLRSAEFFNYTVRTLGLGEEWRGGDVARTVGGGQKVRWLKKEMEKYADREDMIIMFVDSYDVILAGSPTELLKKFVQSGSRLLFSAESFCWPEWGLAEQYPEVGTGKRFLNSGGFIGFATTIHQIVRQWKYKDDDDDQLFYTRLYLDPGLREKLSLNLDHKSRIFQNLNGALDEVVLKFDRNRVRIRNVAYDTLPIVVHGNGPTKLQLNYLGNYVPNGWTPEGGCGFCNQDRRTLPGGQPPPRVFLAVFVEQPTPFLPRFLQRLLLLDYPPDRVTLFLHNNEVFHEPHIADSWPQLQDHFSAVKLVGPEEALSPGEARDMAMDLCRQDPECEFYFSLDADAVLTNLQTLRILIEENRKVIAPMLSRHGKLWSNFWGALSPDEYYARSEDYVELVQRKRVGVWNVPYISQAYVIRGDTLRMELPQRDVFSGSDTDPDMAFCKSFRDKGIFLHLSNQHEFGRLLATSRYDTEHLHPDLWQIFDNPVDWKEQYIHENYSRALEGEGIVEQPCPDVYWFPLLSEQMCDELVAEMEHYGQWSGGRHEDSRLAGGYENVPTVDIHMKQVGYEDQWLQLLRTYVGPMTESLFPGYHTKARAVMNFVVRYRPDEQPSLRPHHDSSTFTLNVALNHKGLDYEGGGCRFLRYDCVISSPRKGWALLHPGRLTHYHEGLPTTWGTRYIMVSFVDP
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O60568 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S23 | Phosphorylation | Uniprot | |
| S25 | Phosphorylation | Uniprot | |
| Y52 | Phosphorylation | Uniprot | |
| N63 | N-Glycosylation | Uniprot | |
| R76 | Methylation | Uniprot | |
| K127 | Acetylation | Uniprot | |
| K128 | Acetylation | Uniprot | |
| K128 | Ubiquitination | Uniprot | |
| S134 | Phosphorylation | Uniprot | |
| T174 | Phosphorylation | Uniprot | |
| T175 | Phosphorylation | Uniprot | |
| K186 | Ubiquitination | Uniprot | |
| K207 | Ubiquitination | Uniprot | |
| K215 | Ubiquitination | Uniprot | |
| K232 | Ubiquitination | Uniprot | |
| T288 | Phosphorylation | Uniprot | |
| S367 | Phosphorylation | Uniprot | |
| R411 | Methylation | Uniprot | |
| S419 | Phosphorylation | Uniprot | |
| Y444 | Phosphorylation | Uniprot | |
| Y460 | Phosphorylation | Uniprot | |
| K495 | Ubiquitination | Uniprot | |
| K500 | Ubiquitination | Uniprot | |
| N548 | N-Glycosylation | Uniprot | |
| K645 | Ubiquitination | Uniprot | |
| Y730 | Phosphorylation | Uniprot |
研究背景
Multifunctional enzyme that catalyzes a series of essential post-translational modifications on Lys residues in procollagen. Plays a redundant role in catalyzing the formation of hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. Plays a redundant role in catalyzing the transfer of galactose onto hydroxylysine groups, giving rise to galactosyl 5-hydroxylysine. Has an essential role by catalyzing the subsequent transfer of glucose moieties, giving rise to 1,2-glucosylgalactosyl-5-hydroxylysine residues. Catalyzes hydroxylation and glycosylation of Lys residues in the MBL1 collagen-like domain, giving rise to hydroxylysine and 1,2-glucosylgalactosyl-5-hydroxylysine residues. Essential for normal biosynthesis and secretion of type IV collagens (Probable). Essential for normal formation of basement membranes (By similarity).
Rough endoplasmic reticulum. Endoplasmic reticulum lumen. Endoplasmic reticulum membrane>Peripheral membrane protein>Lumenal side. Secreted. Secreted>Extracellular space.
Note: The majority of the secreted protein is associated with the extracellular matrix.
Ubiquitous. Detected in heart, placenta and pancreas and at lower levels in lung, liver and skeletal muscle.
Homodimer.
The N-terminal domain mediates glycosyltransferase activity.
The C-terminal domain that mediates lysyl hydroxylase activity is also important for homodimerization.
研究领域
· Metabolism > Amino acid metabolism > Lysine degradation.
· Metabolism > Glycan biosynthesis and metabolism > Other types of O-glycan biosynthesis.
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