产品: Transgelin 2 抗体
货号: DF12053
描述: Rabbit polyclonal antibody to Transgelin 2
应用: WB IHC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 22 kDa; 22kD(Calculated).
蛋白号: P37802
RRID: AB_2844858

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Zebrafish(82%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
克隆:
Polyclonal
特异性:
Transgelin 2 Antibody detects endogenous levels of total Transgelin 2.
RRID:
AB_2844858
引用格式: Affinity Biosciences Cat# DF12053, RRID:AB_2844858.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

CDABP0035; epididymis tissue protein Li 7e; HA1756; KIAA0120; SM22-alpha homolog; TAGL2_HUMAN; Tagln2; Transgelin-2;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
P37802 TAGL2_HUMAN:

Expressed in epididymis (at protein level).

序列:
MANRGPAYGLSREVQQKIEKQYDADLEQILIQWITTQCRKDVGRPQPGRENFQNWLKDGTVLCELINALYPEGQAPVKKIQASTMAFKQMEQISQFLQAAERYGINTTDIFQTVDLWEGKNMACVQRTLMNLGGLAVARDDGLFSGDPNWFPKKSKENPRNFSDNQLQEGKNVIGLQMGTNRGASQAGMTGYGMPRQIL

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Zebrafish
82
Xenopus
75
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P37802 作为底物

Site PTM Type Enzyme
A2 Acetylation
R4 Methylation
Y8 Phosphorylation
S11 Phosphorylation P17252 (PRKCA)
K17 Acetylation
K17 Ubiquitination
K20 Acetylation
K20 Ubiquitination
K40 Ubiquitination
K57 Sumoylation
T60 Phosphorylation
K78 Acetylation
K78 Ubiquitination
K79 Sumoylation
K79 Ubiquitination
S83 Phosphorylation O94921 (CDK14)
T84 Phosphorylation
K88 Acetylation
K88 Ubiquitination
S94 Phosphorylation
T128 Phosphorylation
R139 Methylation
S145 Phosphorylation
K153 Acetylation
K153 Ubiquitination
K154 Ubiquitination
K156 Ubiquitination
S163 Phosphorylation O94921 (CDK14) , P17612 (PRKACA)
K171 Acetylation
K171 Sumoylation
K171 Ubiquitination
T180 Phosphorylation P17252 (PRKCA)
R182 Methylation
S185 Phosphorylation P17252 (PRKCA)
T190 Phosphorylation
Y192 Phosphorylation
R196 Methylation

研究背景

组织特异性:

Expressed in epididymis (at protein level).

蛋白家族:

Belongs to the calponin family.

文献引用

1). Comparative proteomics identify HSP90A, STIP1 and TAGLN‑2 in serum extracellular vesicles as potential circulating biomarkers for human adenomyosis. Experimental and Therapeutic Medicine, 2022 (PubMed: 35495589) [IF=2.7]

Application: WB    Species: human    Sample: T‑AMEVs and B‑AMEVs

Figure 4. | HSP90A, STIP1 and TAGLN‑2 are expressed in B‑AMEVs and T‑AMEVs. (D and E) HSP90A, STIP1 and TAGLN‑2 were present in T‑AMEVs and B‑AMEVs from patients with adenomyosis,but not in those of patients in the control group.

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