SKAP2 Antibody - #DF12085

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产品: SKAP2 抗体
货号: DF12085
描述: Rabbit polyclonal antibody to SKAP2
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 55 kDa; 41kD(Calculated).
蛋白号: O75563
RRID: AB_2844890

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(91%), Horse(91%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(91%)
克隆:
Polyclonal
特异性:
SKAP2 Antibody detects endogenous levels of total SKAP2.
RRID:
AB_2844890
引用格式: Affinity Biosciences Cat# DF12085, RRID:AB_2844890.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Fyn associated phosphoprotein SKAP55 homologue; MGC10411; MGC33; PRAP; Pyk2/RAFTK associated protein; Pyk2/RAFTK-associated protein; RA70; Retinoic acid-induced protein 70; SAPS; SKAP-55HOM; SKAP-HOM; skap2; SKAP2_HUMAN; SKAP55 homolog; SKAP55R; Src associated adaptor protein; Src family associated phosphoprotein 2; Src family-associated phosphoprotein 2; src kinase associated phosphoprotein of 55 related protein; Src kinase-associated phosphoprotein 2; Src kinase-associated phosphoprotein 55-related protein; Src-associated adapter protein with PH and SH3 domains;

抗原和靶标

免疫原:
Uniprot:

O75563(SKAP2_HUMAN) >>Visit HPA database.

基因/基因ID:
SKAP2(8935)
表达:
O75563 SKAP2_HUMAN:

Ubiquitously expressed. Present in platelets (at protein level).

序列:
MPNPSSTSSPYPLPEEIRNLLADVETFVADILKGENLSKKAKEKRESLIKKIKDVKSIYLQEFQDKGDAEDGEEYDDPFAGPPDTISLASERYDKDDEAPSDGAQFPPIAAQDLPFVLKAGYLEKRRKDHSFLGFEWQKRWCALSKTVFYYYGSDKDKQQKGEFAIDGYSVRMNNTLRKDGKKDCCFEISAPDKRIYQFTAASPKDAEEWVQQLKFVLQDMESDIIPEDYDERGELYDDVDHPLPISNPLTSSQPIDDEIYEELPEEEEDSAPVKVEEQRKMSQDSVHHTSGDKSTDYANFYQGLWDCTGAFSDELSFKRGDVIYILSKEYNRYGWWVGEMKGAIGLVPKAYIMEMYDI

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Horse
91
Bovine
91
Xenopus
91
Zebrafish
55
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - O75563 作为底物

Site PTM Type Enzyme
S5 Phosphorylation
S6 Phosphorylation
T7 Phosphorylation
S9 Phosphorylation
Y11 Phosphorylation
S47 Phosphorylation
K56 Ubiquitination
K66 Ubiquitination
Y75 Phosphorylation
T85 Phosphorylation
K119 Ubiquitination
Y122 Phosphorylation
K125 Ubiquitination
T147 Phosphorylation
Y151 Phosphorylation
Y152 Phosphorylation
S154 Phosphorylation
K194 Ubiquitination
Y197 Phosphorylation
S203 Phosphorylation
K205 Ubiquitination
Y237 Phosphorylation
S253 Phosphorylation
Y261 Phosphorylation
K281 Ubiquitination
S283 Phosphorylation
S286 Phosphorylation
T290 Phosphorylation
Y325 Phosphorylation
S328 Phosphorylation
K329 Ubiquitination
Y331 Phosphorylation
Y334 Phosphorylation
K350 Ubiquitination
Y357 Phosphorylation

研究背景

功能:

May be involved in B-cell and macrophage adhesion processes. In B-cells, may act by coupling the B-cell receptor (BCR) to integrin activation. May play a role in src signaling pathway.

翻译修饰:

Phosphorylated in resting platelets. Phosphorylated by FYN on Tyr-261 upon T-cell activation (Probable). Dephosphorylated on Tyr-75 by PTPN22.

细胞定位:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Ubiquitously expressed. Present in platelets (at protein level).

亚基结构:

Homodimer (By similarity). Interacts with PTPNS1. Part of a complex consisting of SKAP2, FYB1 and PTPNS1. Part of a complex consisting of SKAP2, FYB1 and LILRB3. May interact with actin (By similarity). Interacts with FYB1, which is required for SKAP2 protein stability. Interacts with LAT, GRB2, PTK2B and PRAM1. May interact with FYN, HCK and LYN. Interacts with FASLG.

蛋白家族:

The SH3 domain interacts with FYB1 and PTK2B.

Belongs to the SKAP family.

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