TET2 Antibody - #DF12089

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产品: TET2 抗体
货号: DF12089
描述: Rabbit polyclonal antibody to TET2
应用: WB IHC
文献验证: WB
反应: Human, Mouse, Rat
预测: Zebrafish, Bovine, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 130 kDa,133 kDa,224 kDa; 224kD(Calculated).
蛋白号: Q6N021
RRID: AB_2844894

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 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
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MS Report
HPLC Report
MSDS
说明书
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Zebrafish(100%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
克隆:
Polyclonal
特异性:
TET2 Antibody detects endogenous levels of total TET2.
RRID:
AB_2844894
引用格式: Affinity Biosciences Cat# DF12089, RRID:AB_2844894.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

FLJ20032; KIAA1546; MDS; Methylcytosine dioxygenase TET2; Nbla00191; Probable methylcytosine dioxygenase TET2; Protein Ayu17 449; Tet 2; Tet methylcytosine dioxygenase 2; Tet oncogene 2; Tet oncogene family member 2; TET2; TET2_HUMAN;

抗原和靶标

免疫原:
Uniprot:

Q6N021(TET2_HUMAN) >>Visit HPA database.

基因/基因ID:
TET2(54790)
表达:
Q6N021 TET2_HUMAN:

Broadly expressed. Highly expressed in hematopoietic cells; highest expression observed in granulocytes. Expression is reduced in granulocytes from peripheral blood of patients affected by myelodysplastic syndromes.

序列:
MEQDRTNHVEGNRLSPFLIPSPPICQTEPLATKLQNGSPLPERAHPEVNGDTKWHSFKSYYGIPCMKGSQNSRVSPDFTQESRGYSKCLQNGGIKRTVSEPSLSGLLQIKKLKQDQKANGERRNFGVSQERNPGESSQPNVSDLSDKKESVSSVAQENAVKDFTSFSTHNCSGPENPELQILNEQEGKSANYHDKNIVLLKNKAVLMPNGATVSASSVEHTHGELLEKTLSQYYPDCVSIAVQKTTSHINAINSQATNELSCEITHPSHTSGQINSAQTSNSELPPKPAAVVSEACDADDADNASKLAAMLNTCSFQKPEQLQQQKSVFEICPSPAENNIQGTTKLASGEEFCSGSSSNLQAPGGSSERYLKQNEMNGAYFKQSSVFTKDSFSATTTPPPPSQLLLSPPPPLPQVPQLPSEGKSTLNGGVLEEHHHYPNQSNTTLLREVKIEGKPEAPPSQSPNPSTHVCSPSPMLSERPQNNCVNRNDIQTAGTMTVPLCSEKTRPMSEHLKHNPPIFGSSGELQDNCQQLMRNKEQEILKGRDKEQTRDLVPPTQHYLKPGWIELKAPRFHQAESHLKRNEASLPSILQYQPNLSNQMTSKQYTGNSNMPGGLPRQAYTQKTTQLEHKSQMYQVEMNQGQSQGTVDQHLQFQKPSHQVHFSKTDHLPKAHVQSLCGTRFHFQQRADSQTEKLMSPVLKQHLNQQASETEPFSNSHLLQHKPHKQAAQTQPSQSSHLPQNQQQQQKLQIKNKEEILQTFPHPQSNNDQQREGSFFGQTKVEECFHGENQYSKSSEFETHNVQMGLEEVQNINRRNSPYSQTMKSSACKIQVSCSNNTHLVSENKEQTTHPELFAGNKTQNLHHMQYFPNNVIPKQDLLHRCFQEQEQKSQQASVLQGYKNRNQDMSGQQAAQLAQQRYLIHNHANVFPVPDQGGSHTQTPPQKDTQKHAALRWHLLQKQEQQQTQQPQTESCHSQMHRPIKVEPGCKPHACMHTAPPENKTWKKVTKQENPPASCDNVQQKSIIETMEQHLKQFHAKSLFDHKALTLKSQKQVKVEMSGPVTVLTRQTTAAELDSHTPALEQQTTSSEKTPTKRTAASVLNNFIESPSKLLDTPIKNLLDTPVKTQYDFPSCRCVEQIIEKDEGPFYTHLGAGPNVAAIREIMEERFGQKGKAIRIERVIYTGKEGKSSQGCPIAKWVVRRSSSEEKLLCLVRERAGHTCEAAVIVILILVWEGIPLSLADKLYSELTETLRKYGTLTNRRCALNEERTCACQGLDPETCGASFSFGCSWSMYYNGCKFARSKIPRKFKLLGDDPKEEEKLESHLQNLSTLMAPTYKKLAPDAYNNQIEYEHRAPECRLGLKEGRPFSGVTACLDFCAHAHRDLHNMQNGSTLVCTLTREDNREFGGKPEDEQLHVLPLYKVSDVDEFGSVEAQEEKKRSGAIQVLSSFRRKVRMLAEPVKTCRQRKLEAKKAAAEKLSSLENSSNKNEKEKSAPSRTKQTENASQAKQLAELLRLSGPVMQQSQQPQPLQKQPPQPQQQQRPQQQQPHHPQTESVNSYSASGSTNPYMRRPNPVSPYPNSSHTSDIYGSTSPMNFYSTSSQAAGSYLNSSNPMNPYPGLLNQNTQYPSYQCNGNLSVDNCSPYLGSYSPQSQPMDLYRYPSQDPLSKLSLPPIHTLYQPRFGNSQSFTSKYLGYGNQNMQGDGFSSCTIRPNVHHVGKLPPYPTHEMDGHFMGATSRLPPNLSNPNMDYKNGEHHSPSHIIHNYSAAPGMFNSSLHALHLQNKENDMLSHTANGLSKMLPALNHDRTACVQGGLHKLSDANGQEKQPLALVQGVASGAEDNDEVWSDSEQSFLDPDIGGVAVAPTHGSILIECAKRELHATTPLKNPNRNHPTRISLVFYQHKSMNEPKHGLALWEAKMAEKAREKEEECEKYGPDYVPQKSHGKKVKREPAEPHETSEPTYLRFIKSLAERTMSVTTDSTVTTSPYAFTRVTGPYNRYI

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Pig
0
Horse
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q6N021 作为底物

Site PTM Type Enzyme
S15 Phosphorylation
S21 Phosphorylation
S38 Phosphorylation
K53 Acetylation
S75 Phosphorylation
T97 Phosphorylation
S99 Phosphorylation Q13131 (PRKAA1)
S102 Phosphorylation
K110 Acetylation
K111 Acetylation
S334 Phosphorylation
R369 Methylation
S585 Phosphorylation
K655 Ubiquitination
S696 Phosphorylation
K747 Ubiquitination
S817 Phosphorylation
Y819 Phosphorylation
S820 Phosphorylation
T822 Phosphorylation
T859 Phosphorylation
K900 Ubiquitination
S1099 Phosphorylation
S1107 Phosphorylation
S1109 Phosphorylation
T1114 Phosphorylation
K1117 Acetylation
T1122 Phosphorylation
K1299 Ubiquitination
K1317 Acetylation
T1331 Phosphorylation
K1338 Ubiquitination
K1339 Acetylation
K1339 Ubiquitination
Y1345 Phosphorylation
S1449 Phosphorylation
T1463 Phosphorylation
K1478 Acetylation
S1481 Phosphorylation
S1494 Phosphorylation
S1497 Phosphorylation
T1502 Phosphorylation
S1506 Phosphorylation
S1518 O-Glycosylation
S1559 Phosphorylation
Y1560 Phosphorylation
S1561 Phosphorylation
R1682 Methylation
T1975 Phosphorylation

研究背景

功能:

Dioxygenase that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) and plays a key role in active DNA demethylation. Has a preference for 5-hydroxymethylcytosine in CpG motifs. Also mediates subsequent conversion of 5hmC into 5-formylcytosine (5fC), and conversion of 5fC to 5-carboxylcytosine (5caC). Conversion of 5mC into 5hmC, 5fC and 5caC probably constitutes the first step in cytosine demethylation. Methylation at the C5 position of cytosine bases is an epigenetic modification of the mammalian genome which plays an important role in transcriptional regulation. In addition to its role in DNA demethylation, also involved in the recruitment of the O-GlcNAc transferase OGT to CpG-rich transcription start sites of active genes, thereby promoting histone H2B GlcNAcylation by OGT.

翻译修饰:

May be glycosylated. It is unclear whether interaction with OGT leads to GlcNAcylation. According to a report, it is not GlcNAcylated by OGT. In contrast, another group reports GlcNAcylation by OGT in mouse ortholog.

组织特异性:

Broadly expressed. Highly expressed in hematopoietic cells; highest expression observed in granulocytes. Expression is reduced in granulocytes from peripheral blood of patients affected by myelodysplastic syndromes.

亚基结构:

Interacts with HCFC1 and OGT.

蛋白家族:

Belongs to the TET family.

文献引用

1). Activation of prep expression by Tet2 promotes the proliferation of bipotential progenitor cells during liver regeneration. Development (Cambridge, England), 2025 (PubMed: 39976298) [IF=3.7]
2). CircDIP2C ameliorates oxidized low-density lipoprotein-induced cell dysfunction by binding to miR-556-5p to induce TET2 in human umbilical vein endothelial cells. VASCULAR PHARMACOLOGY, 2021 (PubMed: 34147657) [IF=3.5]

Application: WB    Species: Human    Sample: umbilical vein endothelial cells

Fig. 5. TET2 expression was modulated by circDIP2C through functioning as a target gene of miR-556-5p in HUVECs. (A) The binding sites between miR-556-5p and TET2 were predicted by targetscan database. (B-D) The binding relationship between miR-556-5p and TET2 was proved by dual-luciferase reporter, RIP and RNA pull-down assays in HUVECs. (E and F) The mRNA and protein expression of TET2 were detected by qRT-PCR or western blot analysis in the HUVECs transfected with miR-NC or miR-556-5p. (G and H) The effects between circDIP2C and miR-556-5p overexpression on the mRNA and protein expression of TET2 were determined by qRT-PCR or western blot analysis in HUVECs. (I and J) The impacts of ox-LDL on the mRNA and protein expression of TET2 were demonstrated by qRT-PCR and western blot analysis, respectively, in HUVECs. *P < 0.05.

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