产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF12092, RRID:AB_2844897.
展开/折叠
Adapter related protein complex 3 beta 1 subunit; Adapter-related protein complex 3 subunit beta-1; Adaptor protein complex AP-3 subunit beta-1; Adaptor protein complex AP3 beta1 subunit; ADTB3; ADTB3A; AP-3 complex subunit beta-1; AP3 complex beta1 subunit; AP3B1; AP3B1_HUMAN; Beta-3A-adaptin; Beta3A adaptin; Clathrin assembly protein complex 3 beta 1 large chain; Clathrin assembly protein complex 3 beta-1 large chain; HPS; PE;
抗原和靶标
- O00203 AP3B1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSSNSFPYNEQSGGGEATELGQEATSTISPSGAFGLFSSDLKKNEDLKQMLESNKDSAKLDAMKRIVGMIAKGKNASELFPAVVKNVASKNIEIKKLVYVYLVRYAEEQQDLALLSISTFQRALKDPNQLIRASALRVLSSIRVPIIVPIMMLAIKEASADLSPYVRKNAAHAIQKLYSLDPEQKEMLIEVIEKLLKDKSTLVAGSVVMAFEEVCPDRIDLIHKNYRKLCNLLVDVEEWGQVVIIHMLTRYARTQFVSPWKEGDELEDNGKNFYESDDDQKEKTDKKKKPYTMDPDHRLLIRNTKPLLQSRNAAVVMAVAQLYWHISPKSEAGIISKSLVRLLRSNREVQYIVLQNIATMSIQRKGMFEPYLKSFYVRSTDPTMIKTLKLEILTNLANEANISTLLREFQTYVKSQDKQFAAATIQTIGRCATNILEVTDTCLNGLVCLLSNRDEIVVAESVVVIKKLLQMQPAQHGEIIKHMAKLLDSITVPVARASILWLIGENCERVPKIAPDVLRKMAKSFTSEDDLVKLQILNLGAKLYLTNSKQTKLLTQYILNLGKYDQNYDIRDRTRFIRQLIVPNVKSGALSKYAKKIFLAQKPAPLLESPFKDRDHFQLGTLSHTLNIKATGYLELSNWPEVAPDPSVRNVEVIELAKEWTPAGKAKQENSAKKFYSESEEEEDSSDSSSDSESESGSESGEQGESGEEGDSNEDSSEDSSSEQDSESGRESGLENKRTAKRNSKAKGKSDSEDGEKENEKSKTSDSSNDESSSIEDSSSDSESESEPESESESRRVTKEKEKKTKQDRTPLTKDVSLLDLDDFNPVSTPVALPTPALSPSLMADLEGLHLSTSSSVISVSTPAFVPTKTHVLLHRMSGKGLAAHYFFPRQPCIFGDKMVSIQITLNNTTDRKIENIHIGEKKLPIGMKMHVFNPIDSLEPEGSITVSMGIDFCDSTQTASFQLCTKDDCFNVNIQPPVGELLLPVAMSEKDFKKEQGVLTGMNETSAVIIAAPQNFTPSVIFQKVVNVANVGAVPSGQDNIHRFAAKTVHSGSLMLVTVELKEGSTAQLIINTEKTVIGSVLLRELKPVLSQG
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O00203 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S89 | Phosphorylation | Uniprot | |
| K90 | Ubiquitination | Uniprot | |
| K125 | Ubiquitination | Uniprot | |
| S140 | Phosphorylation | Uniprot | |
| S141 | Phosphorylation | Uniprot | |
| K168 | Ubiquitination | Uniprot | |
| K176 | Ubiquitination | Uniprot | |
| S200 | Phosphorylation | Uniprot | |
| S206 | Phosphorylation | Uniprot | |
| Y274 | Phosphorylation | Uniprot | |
| S276 | Phosphorylation | Uniprot | |
| T292 | Phosphorylation | Uniprot | |
| K414 | Ubiquitination | Uniprot | |
| K467 | Ubiquitination | Uniprot | |
| K512 | Ubiquitination | Uniprot | |
| R519 | Methylation | Uniprot | |
| K523 | Ubiquitination | Uniprot | |
| S527 | Phosphorylation | Uniprot | |
| K533 | Ubiquitination | Uniprot | |
| K549 | Ubiquitination | Uniprot | |
| K552 | Ubiquitination | Uniprot | |
| T555 | Phosphorylation | Uniprot | |
| Y557 | Phosphorylation | Uniprot | |
| K563 | Acetylation | Uniprot | |
| K592 | Acetylation | Uniprot | |
| K592 | Ubiquitination | Uniprot | |
| K596 | Ubiquitination | Uniprot | |
| K602 | Ubiquitination | Uniprot | |
| S609 | Phosphorylation | Uniprot | |
| T621 | Phosphorylation | Uniprot | |
| S647 | Phosphorylation | Uniprot | |
| K658 | Ubiquitination | Uniprot | |
| T661 | Phosphorylation | Uniprot | |
| K665 | Ubiquitination | Uniprot | |
| S750 | Phosphorylation | Uniprot | |
| S752 | Phosphorylation | Uniprot | |
| T810 | Phosphorylation | Uniprot | |
| T862 | Phosphorylation | Uniprot | |
| T1077 | Phosphorylation | Uniprot | |
| S1081 | Phosphorylation | Uniprot | |
| S1092 | Phosphorylation | Q13535 (ATR) | Uniprot |
研究背景
Subunit of non-clathrin- and clathrin-associated adaptor protein complex 3 (AP-3) that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules. AP-3 appears to be involved in the sorting of a subset of transmembrane proteins targeted to lysosomes and lysosome-related organelles. In concert with the BLOC-1 complex, AP-3 is required to target cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals.
Phosphorylated on serine residues.
Cytoplasmic vesicle>Clathrin-coated vesicle membrane>Peripheral membrane protein>Cytoplasmic side. Golgi apparatus.
Note: Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex.
Ubiquitously expressed.
AP-3 associates with the BLOC-1 complex (By similarity). Adaptor protein complex 3 (AP-3) is a heterotetramer composed of two large adaptins (delta-type subunit AP3D1 and beta-type subunit AP3B1 or AP3B2), a medium adaptin (mu-type subunit AP3M1 or AP3M2) and a small adaptin (sigma-type subunit APS1 or AP3S2).
Belongs to the adaptor complexes large subunit family.
研究领域
· Cellular Processes > Transport and catabolism > Lysosome. (View pathway)
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