产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF12096, RRID:AB_2844901.
展开/折叠
1500009M05Rik; 1500026J14Rik; 1500031D15Rik; AI848398; B630006A20Rik; CDGSH iron sulfur domain 2; CDGSH iron-sulfur domain-containing protein 2; CDGSH type domain 2; CISD2; CISD2_HUMAN; Endoplasmic reticulum intermembrane small protein; ERIS; Miner1; MitoNEET related 1; MitoNEET-related 1 protein; NAF-1; Noxp70; Nutrient deprivation autophagy factor 1; Nutrient-deprivation autophagy factor-1; OTTHUMP00000219576; RGD1566242; WFS2; Zcd2; Zinc finger; Zinc finger, CDGSH type domain 2;
抗原和靶标
- Q8N5K1 CISD2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MVLESVARIVKVQLPAYLKRLPVPESITGFARLTVSEWLRLLPFLGVLALLGYLAVRPFLPKKKQQKDSLINLKIQKENPKVVNEINIEDLCLTKAAYCRCWRSKTFPACDGSHNKHNELTGDNVGPLILKKKEV
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q8N5K1 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K19 | Ubiquitination | Uniprot | |
| S36 | Phosphorylation | Uniprot | |
| K67 | Ubiquitination | Uniprot | |
| S69 | Phosphorylation | Uniprot | |
| K74 | Ubiquitination | Uniprot | |
| K81 | Ubiquitination | Uniprot | |
| K95 | Ubiquitination | Uniprot | |
| K105 | Ubiquitination | Uniprot | |
| T106 | Phosphorylation | Uniprot | |
| K131 | Methylation | Uniprot | |
| K132 | Methylation | Uniprot |
研究背景
Regulator of autophagy that contributes to antagonize BECN1-mediated cellular autophagy at the endoplasmic reticulum. Participates in the interaction of BCL2 with BECN1 and is required for BCL2-mediated depression of endoplasmic reticulum Ca(2+) stores during autophagy. Contributes to BIK-initiated autophagy, while it is not involved in BIK-dependent activation of caspases. Involved in life span control, probably via its function as regulator of autophagy.
Endoplasmic reticulum membrane>Single-pass membrane protein. Mitochondrion outer membrane>Single-pass membrane protein.
Note: According to PubMed:20010695, it mainly localizes to the endoplasmic reticulum. However, experiments in mouse showed that it mainly localizes to the mitochondrion outer membrane.
Testis, small intestine, kidney, lung, brain, heart, pancreas and platelets.
Homodimer. Interacts with BCL2; the interaction is direct and disrupted by BIK interaction with BCL2. Interacts with BCL2L1. Interacts with ITPR1.
Belongs to the CISD protein family. CISD2 subfamily.
限制条款
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