RDH10 Antibody - #DF12105

Fig. 6. A. muciniphila affects spermatogenesis by regulating retinol metabolism. (A) Metabolite pathways annotated for all serum metabolites correlated with Akkermansia based on the KEGG Mapper database. (B) Relative serum retinol expression levels in the Non-HS and HS groups were examined using HPLC (n = 8). (C) The relative serum and testicular retinol levels in the ABX-FMT-Non-HS and ABX-FMT-HS groups were measured using an ELISA kit (n = 5). (D) The relative serum and testicular retinol levels in the three groups were measured using an ELISA kit (n = 5). (E) Western blot of RDH10 and β-ACTIN in the two groups (n = 3). (F) Quantification of RDH10 protein levels. (G) Representative images of RDH10 IF staining in testicular tissue sections. Scale bar = 50 μm. (H) Western blot of RDH10 and β-ACTIN in the three groups (n = 3). (I) Quantification of RDH10 protein levels. (J) The relative testicular RA expression levels in the three groups were measured using an ELISA kit (n = 5).

Fig. 6. A. muciniphila affects spermatogenesis by regulating retinol metabolism. (A) Metabolite pathways annotated for all serum metabolites correlated with Akkermansia based on the KEGG Mapper database. (B) Relative serum retinol expression levels in the Non-HS and HS groups were examined using HPLC (n = 8). (C) The relative serum and testicular retinol levels in the ABX-FMT-Non-HS and ABX-FMT-HS groups were measured using an ELISA kit (n = 5). (D) The relative serum and testicular retinol levels in the three groups were measured using an ELISA kit (n = 5). (E) Western blot of RDH10 and β-ACTIN in the two groups (n = 3). (F) Quantification of RDH10 protein levels. (G) Representative images of RDH10 IF staining in testicular tissue sections. Scale bar = 50 μm. (H) Western blot of RDH10 and β-ACTIN in the three groups (n = 3). (I) Quantification of RDH10 protein levels. (J) The relative testicular RA expression levels in the three groups were measured using an ELISA kit (n = 5).

Fig. 5 Retinoic acid could rescue the spermatogenesis dysfunction caused by NSC319726. (A) Retinol and retinoic acid levels in testis (n = 5). (B) Schematic of retinoic acid initiating meiosis. (C) Immunofluorescence staining of STRA8 and RDH10 in testis. (D) Flowchart of medication administration. (E) HE staining of testis tissue sections. (red arrow: Es, elongated spermatids; Sg, spermatogonia; Rs, round spermatid; Sc, spermatocyte). (F) Testis index, sperm count per epididymis, sperm malformation, and pregnancy rate. (G) Western blot of DDX4, STRA8, RDH10, and SYCP3 in spermatogenesis (n = 3). (H) Quantification of Western blot gray-scale

Figure 4. Immunocytochemical evaluation of CHD3 (green), RDH10 (green), NTM (green), and αSMA (red) in the control (Control) MSCs and after incubation in profibrotic (F) or adipogenic conditions (Ad). Fluorescent microscopy, blue staining—nuclei stained with DAPI, scale bar is 50 µm (A). The percentage of CHD3-positive cells, mean ± SE, p value is indicated (B). The percentage of cells with neurotrimin clusters, mean ± SE, p value is indicated (C).