产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF12123, RRID:AB_2844928.
展开/折叠
N acetyl alpha neuraminidase 4; Neuraminidase 4; Sialidase 4;
抗原和靶标
- Q8WWR8 NEUR4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGVPRTPSRTVLFERERTGLTYRVPSLLPVPPGPTLLAFVEQRLSPDDSHAHRLVLRRGTLAGGSVRWGALHVLGTAALAEHRSMNPCPVHDAGTGTVFLFFIAVLGHTPEAVQIATGRNAARLCCVASRDAGLSWGSARDLTEEAIGGAVQDWATFAVGPGHGVQLPSGRLLVPAYTYRVDRRECFGKICRTSPHSFAFYSDDHGRTWRCGGLVPNLRSGECQLAAVDGGQAGSFLYCNARSPLGSRVQALSTDEGTSFLPAERVASLPETAWGCQGSIVGFPAPAPNRPRDDSWSVGPGSPLQPPLLGPGVHEPPEEAAVDPRGGQVPGGPFSRLQPRGDGPRQPGPRPGVSGDVGSWTLALPMPFAAPPQSPTWLLYSHPVGRRARLHMGIRLSQSPLDPRSWTEPWVIYEGPSGYSDLASIGPAPEGGLVFACLYESGARTSYDEISFCTFSLREVLENVPASPKPPNLGDKPRGCCWPS
翻译修饰 - Q8WWR8 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T10 | Phosphorylation | Uniprot | |
| T18 | Phosphorylation | Uniprot | |
| T21 | Phosphorylation | Uniprot | |
| Y22 | Phosphorylation | Uniprot | |
| T193 | Phosphorylation | Uniprot | |
| S194 | Phosphorylation | Uniprot | |
| S197 | Phosphorylation | Uniprot | |
| S202 | Phosphorylation | Uniprot | |
| S335 | Phosphorylation | Uniprot |
研究背景
May function in lysosomal catabolism of sialylated glycoconjugates. Has sialidase activity towards synthetic substrates, such as 2'-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid (4-MU-NANA or 4MU-NeuAc). Has a broad substrate specificity being active on glycoproteins, oligosaccharides and sialylated glycolipids.
According to phosphorylation of mannose residues may ensure efficient transport of isoform 2 to the lysosomes via the mannose 6-phosphate receptor.
Isoform 2 is glycosylated.
Membrane>Peripheral membrane protein.
Lysosome lumen.
Note: According to PubMed:15213228, isoform 2 is soluble, N-glycosylated and found in the lumen of lysosomes. However, no signal sequence nor N-glycosylation site is predicted from the sequence.
Ubiquitous with higher expression in heart, skeletal muscle, liver and placenta.
Belongs to the glycosyl hydrolase 33 family.
研究领域
· Metabolism > Glycan biosynthesis and metabolism > Other glycan degradation.
· Metabolism > Lipid metabolism > Sphingolipid metabolism.
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