NOD2 Antibody - #DF12125

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产品: NOD2 抗体
货号: DF12125
描述: Rabbit polyclonal antibody to NOD2
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit
分子量: 100-110 kDa; 115kD(Calculated).
蛋白号: Q9HC29
RRID: AB_2844930

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实验方案
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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(92%), Bovine(100%), Horse(92%), Sheep(100%), Rabbit(92%)
克隆:
Polyclonal
特异性:
NOD2 Antibody detects endogenous levels of total NOD2.
RRID:
AB_2844930
引用格式: Affinity Biosciences Cat# DF12125, RRID:AB_2844930.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

ACUG; Arthrocutaneouveal granulomatosis; BLAU; CARD15; Caspase recruitment domain family, member 15; Caspase recruitment domain protein 15; Caspase recruitment domain-containing protein 15; CD; CLR16.3; IBD1; Inflammatory bowel disease protein 1; LRR containing protein; NLR family, CARD domain containing 2; NLRC2; NOD like receptor C2; NOD2; NOD2 protein; NOD2_HUMAN; NOD2B; nucleotide binding oligomerization domain 2; Nucleotide binding oligomerization domain containing 2; Nucleotide binding oligomerization domain, leucine rich repeat and CARD domain containing 2; Nucleotide-binding oligomerization domain-containing protein 2; PSORAS1;

抗原和靶标

免疫原:
Uniprot:

Q9HC29(NOD2_HUMAN) >>Visit HPA database.

基因/基因ID:
NOD2(64127)
表达:
Q9HC29 NOD2_HUMAN:

Expressed in intestinal mucosa, mainly in Paneth cells and, at lower extent, in the glandular epithelium.

序列:
MGEEGGSASHDEEERASVLLGHSPGCEMCSQEAFQAQRSQLVELLVSGSLEGFESVLDWLLSWEVLSWEDYEGFHLLGQPLSHLARRLLDTVWNKGTWACQKLIAAAQEAQADSQSPKLHGCWDPHSLHPARDLQSHRPAIVRRLHSHVENMLDLAWERGFVSQYECDEIRLPIFTPSQRARRLLDLATVKANGLAAFLLQHVQELPVPLALPLEAATCKKYMAKLRTTVSAQSRFLSTYDGAETLCLEDIYTENVLEVWADVGMAGPPQKSPATLGLEELFSTPGHLNDDADTVLVVGEAGSGKSTLLQRLHLLWAAGQDFQEFLFVFPFSCRQLQCMAKPLSVRTLLFEHCCWPDVGQEDIFQLLLDHPDRVLLTFDGFDEFKFRFTDRERHCSPTDPTSVQTLLFNLLQGNLLKNARKVVTSRPAAVSAFLRKYIRTEFNLKGFSEQGIELYLRKRHHEPGVADRLIRLLQETSALHGLCHLPVFSWMVSKCHQELLLQEGGSPKTTTDMYLLILQHFLLHATPPDSASQGLGPSLLRGRLPTLLHLGRLALWGLGMCCYVFSAQQLQAAQVSPDDISLGFLVRAKGVVPGSTAPLEFLHITFQCFFAAFYLALSADVPPALLRHLFNCGRPGNSPMARLLPTMCIQASEGKDSSVAALLQKAEPHNLQITAAFLAGLLSREHWGLLAECQTSEKALLRRQACARWCLARSLRKHFHSIPPAAPGEAKSVHAMPGFIWLIRSLYEMQEERLARKAARGLNVGHLKLTFCSVGPTECAALAFVLQHLRRPVALQLDYNSVGDIGVEQLLPCLGVCKALYLRDNNISDRGICKLIECALHCEQLQKLALFNNKLTDGCAHSMAKLLACRQNFLALRLGNNYITAAGAQVLAEGLRGNTSLQFLGFWGNRVGDEGAQALAEALGDHQSLRWLSLVGNNIGSVGAQALALMLAKNVMLEELCLEENHLQDEGVCSLAEGLKKNSSLKILKLSNNCITYLGAEALLQALERNDTILEVWLRGNTFSLEEVDKLGCRDTRLLL

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Bovine
100
Sheep
100
Pig
92
Horse
92
Rabbit
92
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q9HC29 作为底物

Site PTM Type Enzyme
T189 Phosphorylation
S238 Phosphorylation
T239 Phosphorylation
S402 Phosphorylation
T424 Phosphorylation
S425 Phosphorylation
S477 Phosphorylation
S493 Phosphorylation
K655 Ubiquitination
K717 Acetylation
K731 Acetylation
S745 Phosphorylation
Y747 Phosphorylation
S983 Phosphorylation
S984 Phosphorylation
T1022 Phosphorylation
S1024 Phosphorylation

研究背景

功能:

Involved in gastrointestinal immunity. Upon stimulation by muramyl dipeptide (MDP), a fragment of bacterial peptidoglycan, binds the proximal adapter receptor-interacting RIPK2, which recruits ubiquitin ligases as XIAP, BIRC2, BIRC3, INAVA and the LUBAC complex, triggering activation of MAP kinases and activation of NF-kappa-B signaling. This in turn leads to the transcriptional activation of hundreds of genes involved in immune response. Required for MDP-induced NLRP1-dependent CASP1 activation and IL1B release in macrophages. Component of an autophagy-mediated antibacterial pathway together with ATG16L1. Plays also a role in sensing single-stranded RNA (ssRNA) from viruses. Interacts with mitochondrial antiviral signaling/MAVS, leading to activation of interferon regulatory factor-3/IRF3 and expression of type I interferon.

翻译修饰:

Polyubiquitinated following MDP stimulation, leading to proteasome-mediated degradation.

细胞定位:

Cytoplasm. Membrane. Mitochondrion. Basolateral cell membrane.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed in intestinal mucosa, mainly in Paneth cells and, at lower extent, in the glandular epithelium.

亚基结构:

Component of a signaling complex consisting of ARHGEF2, NOD2 and RIPK2. Interacts (via CARD domain) with RIPK2 (via CARD domain). Interacts with ATG16L1. Interacts (via NACHT domain) with CARD9. Interacts with ANKRD17 (via N-terminus). Interacts with HSPA1A; the interaction enhances NOD2 stability. Interacts (via both CARD domains) with HSP90; the interaction enhances NOD2 stability. Interacts (via CARD domain) with SOCS3; the interaction promotes NOD2 degradation. Interacts (via CARD domain) with ERBBI2P; the interaction inhibits activation of NOD2. Interacts (via CARD domain) with CASP1; this interaction leads to IL1B processing. Also interacts with CASP4. Interacts with NLRP1; this interaction is enhanced in the presence of muramyl dipeptide (MDP) and leads to increased IL1B release. Interacts with MAPKBP1; the interaction is enhanced in the presence of muramyl dipeptide (MDP). Interacts with INAVA; the interaction takes place upon PRR stimulation. Interacts with ANKHD1, C10ORF67, CHMP5, DOCK7, ENTR1, KRT15, LDOC1, PPP1R12C, PPP2R3B, TRIM41 and VIM. Interacts with NLRP12; this interaction promotes degradation of NOD2 through the ubiquitin-proteasome pathway.

蛋白家族:

The ATG16L1-binding motif mediates interaction with ATG16L1.

Intramolecular interactions between the N-terminal moiety and the leucine-rich repeats (LRR) may be important for autoinhibition in the absence of activating signal. In the absence of LRRs, the protein becomes a constitutive activator of CASP1 cleavage and proIL1B processing.

研究领域

· Environmental Information Processing > Signal transduction > TNF signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Shigellosis.

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

· Human Diseases > Immune diseases > Inflammatory bowel disease (IBD).

· Organismal Systems > Immune system > NOD-like receptor signaling pathway.   (View pathway)

文献引用

1). Cytosolic HMGB1 Mediates LPS-Induced Autophagy in Microglia by Interacting with NOD2 and Suppresses Its Proinflammatory Function. Cells (PubMed: 35954253) [IF=6.0]
2). Interferon-γ activated T-cell IRGM–autophagy axis in oral lichen planus. International Immunopharmacology (PubMed: 33639564) [IF=5.6]

Application: WB    Species: Human    Sample: peripheral blood T cells

Fig. 3. Upregulated expression of IRGM and autophagy in peripheral blood T cells of OLP patients. (A-B) Quantitative real-time RT-PCR results showed that IRGM mRNA levels increased in the peripheral blood T cells of OLP (n = 22, P = 0.001), non-erosive OLP (NEOLP, n = 12, P = 0.013) and erosive OLP (EOLP, n = 10, P = 0.033) when compared with that in healthy controls (n = 15), respectively. However, the relative levels of IRGM mRNA showed no significant difference between different clinical types of OLP (P > 0.05). (C-D) Similarly, the relative protein level of IRGM increased in peripheral blood T cells of EOLP (P < 0.001) and NEOLP (P < 0.001) when compared with that in healthy controls. (E-G) Meanwhile, the relative protein level of LC3B upregulated, whereas SQSTM1 and NOD2 protein expression decreased in peripheral blood T cells of all OLP patients (P < 0.05). There were significant differences of expression of LC3B (E, P < 0.001) and SQSTM1 protein (F, P < 0.001) between EOLP and NEOLP groups. The assay was performed in triplicate no less than 3 times. Data were shown as mean ± SEM. * P < 0.05, ** P < 0.01, ***P < 0.001, NS: P > 0.05, nonsignificantly.
3). Novel chimeric TLR2/NOD2 agonist CL429 exhibited significant radioprotective effects in mice. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE (PubMed: 33609010) [IF=5.3]

Application: WB    Species: mice    Sample: intestinal tissues

FIGURE 5 The mechanism for the radioprotective effect of CL429. (A) C57BL/6 mice and TLR2 KO mice were treated with CL429 before TBI, and then, the survival was monitored (WT n = 11, TLR2 KO n = 7). (B) The representative pathological images of haematopoietic system and gastrointestinal tract at 3.5 d after radiation. (C) The protein expression of TLR2 and NOD2 after siRNA knockdown. Cell viability was determined using CCK-8 at 24 h after radiation. *P < .05 vs IR groups. (D) The effects of CL429 on TLR2, NOD2, MyD88 and p-IKK at 0 h, 2 h, 4 h, 8 h, 12 h and 24 h were measured by Western blot assay. (E) IL-6, IL-11, IL-12 and TNF-α were detected by ELISA 24 h after radiation. *P < .05, **P < .01 vs IR + PBS groups
4). An Inflammatory Response-Related Gene Signature Can Impact the Immune Status and Predict the Prognosis of Hepatocellular Carcinoma. Frontiers in Oncology (PubMed: 33828988) [IF=4.7]

Application: IHC    Species: Human    Sample: HCC Tissues and Adjacent Non-Tumorous Tissues

Figure 10 Experiment confirmed the difference of the prognostic gene expression between HCC and adjacent non-tumor tissues. (A) The mRNA expression analysis by qRT-RCR. (B) The protein expression analysis by IHC.

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