PYGL Antibody - #DF12134

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产品: PYGL 抗体
货号: DF12134
描述: Rabbit polyclonal antibody to PYGL
应用: WB IHC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 97 kDa; 97kD(Calculated).
蛋白号: P06737
RRID: AB_2844939

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MS Report
HPLC Report
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说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(92%), Zebrafish(92%), Bovine(92%), Horse(100%), Sheep(92%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
克隆:
Polyclonal
特异性:
PYGL Antibody detects endogenous levels of total PYGL.
RRID:
AB_2844939
引用格式: Affinity Biosciences Cat# DF12134, RRID:AB_2844939.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Glycogen phosphorylase; Glycogen phosphorylase liver; Glycogen phosphorylase liver form; GSD6; Hers disease, glycogen storage disease type VI; liver form; OTTHUMP00000233649; OTTHUMP00000233651; Phosphorylase glycogen liver; Pygl; PYGL_HUMAN;

抗原和靶标

免疫原:
Uniprot:

P06737(PYGL_HUMAN) >>Visit HPA database.

基因/基因ID:
PYGL(5836)
序列:
MAKPLTDQEKRRQISIRGIVGVENVAELKKSFNRHLHFTLVKDRNVATTRDYYFALAHTVRDHLVGRWIRTQQHYYDKCPKRVYYLSLEFYMGRTLQNTMINLGLQNACDEAIYQLGLDIEELEEIEEDAGLGNGGLGRLAACFLDSMATLGLAAYGYGIRYEYGIFNQKIRDGWQVEEADDWLRYGNPWEKSRPEFMLPVHFYGKVEHTNTGTKWIDTQVVLALPYDTPVPGYMNNTVNTMRLWSARAPNDFNLRDFNVGDYIQAVLDRNLAENISRVLYPNDNFFEGKELRLKQEYFVVAATLQDIIRRFKASKFGSTRGAGTVFDAFPDQVAIQLNDTHPALAIPELMRIFVDIEKLPWSKAWELTQKTFAYTNHTVLPEALERWPVDLVEKLLPRHLEIIYEINQKHLDRIVALFPKDVDRLRRMSLIEEEGSKRINMAHLCIVGSHAVNGVAKIHSDIVKTKVFKDFSELEPDKFQNKTNGITPRRWLLLCNPGLAELIAEKIGEDYVKDLSQLTKLHSFLGDDVFLRELAKVKQENKLKFSQFLETEYKVKINPSSMFDVQVKRIHEYKRQLLNCLHVITMYNRIKKDPKKLFVPRTVIIGGKAAPGYHMAKMIIKLITSVADVVNNDPMVGSKLKVIFLENYRVSLAEKVIPATDLSEQISTAGTEASGTGNMKFMLNGALTIGTMDGANVEMAEEAGEENLFIFGMRIDDVAALDKKGYEAKEYYEALPELKLVIDQIDNGFFSPKQPDLFKDIINMLFYHDRFKVFADYEAYVKCQDKVSQLYMNPKAWNTMVLKNIAASGKFSSDRTIKEYAQNIWNVEPSDLKISLSNESNKVNGN

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Horse
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Pig
92
Bovine
92
Sheep
92
Zebrafish
92
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P06737 作为底物

Site PTM Type Enzyme
A2 Acetylation
K3 Ubiquitination
T6 Phosphorylation
K10 Ubiquitination
S15 Phosphorylation
K29 Acetylation
K29 Ubiquitination
K30 Ubiquitination
S31 Phosphorylation
T39 Phosphorylation
K42 Ubiquitination
Y75 Phosphorylation
Y76 Phosphorylation
R82 Methylation
Y85 Phosphorylation
Y114 Phosphorylation
K170 Ubiquitination
Y186 Phosphorylation
K192 Ubiquitination
S193 Phosphorylation
Y204 Phosphorylation
K206 Ubiquitination
R248 Methylation
Y263 Phosphorylation
Y281 Phosphorylation
K290 Ubiquitination
K295 Ubiquitination
K316 Ubiquitination
K359 Ubiquitination
S363 Phosphorylation
K364 Ubiquitination
K371 Ubiquitination
K395 Ubiquitination
Y405 Phosphorylation
K410 Ubiquitination
R414 Methylation
K421 Ubiquitination
S430 Phosphorylation
K438 Ubiquitination
S450 Phosphorylation
K465 Ubiquitination
K470 Acetylation
K470 Ubiquitination
S473 Phosphorylation
K479 Ubiquitination
K483 Ubiquitination
K514 Ubiquitination
S517 Phosphorylation
K521 Ubiquitination
S524 Phosphorylation
K545 Ubiquitination
S547 Phosphorylation
K555 Ubiquitination
K557 Ubiquitination
K569 Ubiquitination
K575 Acetylation
K609 Ubiquitination
S626 Phosphorylation
S639 Phosphorylation
K640 Ubiquitination
K642 Ubiquitination
K656 Ubiquitination
K724 Acetylation
K724 Ubiquitination
K725 Ubiquitination
K730 Ubiquitination
S752 Phosphorylation
K754 Ubiquitination
Y778 Phosphorylation
K783 Ubiquitination
K787 Ubiquitination
K796 Ubiquitination
K804 Ubiquitination
K811 Ubiquitination
R816 Methylation
K819 Ubiquitination
K834 Acetylation
K834 Ubiquitination
S836 Phosphorylation
S838 Phosphorylation
K843 Ubiquitination

研究背景

功能:

Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties.

翻译修饰:

Phosphorylation of Ser-15 converts phosphorylase B (unphosphorylated) to phosphorylase A.

亚基结构:

Homodimer. Dimers associate into a tetramer to form the enzymatically active phosphorylase A. Interacts with PPP1R3B (By similarity).

蛋白家族:

Belongs to the glycogen phosphorylase family.

研究领域

· Cellular Processes > Cell growth and death > Necroptosis.   (View pathway)

· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.

· Metabolism > Carbohydrate metabolism > Starch and sucrose metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Endocrine system > Insulin signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Glucagon signaling pathway.

文献引用

1). Tributyltin exposure disturbs hepatic glucose metabolism in male mice. TOXICOLOGY, 2019 (PubMed: 31306684) [IF=4.5]

Application: WB    Species: mouse    Sample: liver

Fig. 5. |Effect of tributyltin (TBT) on hepatic glucose metabolism in male mice after exposure for 45 days. (A) Representative chemiluminescence detection of protein expression in liver.
2). Analysis of the expression, function and signaling of glycogen phosphorylase isoforms in hepatocellular carcinoma. Oncology Letters, 2022 (PubMed: 35761940) [IF=2.9]

Application: WB    Species: Human    Sample: HCC cell

Figure 1. Analysis of the expression of PYGB, PYGL and PYGM in HCC. (A) Analysis of PYGB, PYGL and PYGM mRNA expression in HCC from UALCAN (http://ualcan.path.uab.edu/analysis.html). Dataset TCGA-LIHC from study accession phs000178.v11.p8 was used. (B) Analysis of PYGB, PYGL and PYGM protein expression in a human HCC cell line (MHCC97H) by western blotting. (C) Promoter methylation level analysis of PYGB, PYGL and PYGM in HCC from UALCAN. The dataset TCGA-LIHC from study accession phs000178.v11.p8 was used. *P
3). Exposure of zebrafish to a cold environment triggered cellular autophagy in zebrafish liver. JOURNAL OF FISH DISEASES, 2022 (PubMed: 35395109) [IF=2.5]
4). Biochemical analysis challenging Western blot analysis as validation step for antibodies intended for ELISA and Immunohistochemistry use. bioRxiv, 2022

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