SEC61G Antibody - #DF12136

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产品: SEC61G 抗体
货号: DF12136
描述: Rabbit polyclonal antibody to SEC61G
应用: WB IHC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Rabbit, Dog, Chicken, Xenopus
分子量: 7.7 kDa; 8kD(Calculated).
蛋白号: P60059
RRID: AB_2844941

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
克隆:
Polyclonal
特异性:
SEC61G Antibody detects endogenous levels of total SEC61G.
RRID:
AB_2844941
引用格式: Affinity Biosciences Cat# DF12136, RRID:AB_2844941.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Protein transport protein Sec61 subunit gamma; SC61G_HUMAN; Sec61 gamma subunit; SEC61G; SSS1;

抗原和靶标

免疫原:
Uniprot:

P60059(SC61G_HUMAN) >>Visit HPA database.

基因/基因ID:
SEC61G(23480)
序列:
MDQVMQFVEPSRQFVKDSIRLVKRCTKPDRKEFQKIAMATAIGFAIMGFIGFFVKLIHIPINNIIVGG

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Sheep
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P60059 作为底物

Site PTM Type Enzyme
M1 Acetylation
K16 Ubiquitination
S18 Phosphorylation

研究背景

功能:

Component of SEC61 channel-forming translocon complex that mediates transport of signal peptide-containing precursor polypeptides across endoplasmic reticulum (ER).

细胞定位:

Endoplasmic reticulum membrane>Single-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

The ER translocon complex consists of channel-forming core components SEC61A1, SEC61B and SEC61G and different auxiliary components such as SEC62 and SEC63.

蛋白家族:

Belongs to the SecE/SEC61-gamma family.

研究领域

· Cellular Processes > Transport and catabolism > Phagosome.   (View pathway)

· Genetic Information Processing > Folding, sorting and degradation > Protein export.

· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Vibrio cholerae infection.

文献引用

1). Identification of dihydrotanshinone I as an ERp57 inhibitor with anti-breast cancer properties via the UPR pathway. BIOCHEMICAL PHARMACOLOGY, 2021 (PubMed: 34062127) [IF=5.8]

Application: WB    Species: Human    Sample: breast cancer cell

Fig. 5. DHT activated ER stress and UPR signaling pathways. MDA-MB-231 cells were incubated with 3 μ M DHT for the indicated time. (A) Western blot analysis was performed with antibodies against Sec61γ, BiP, p-PERK/PERK, p-eIF2 α /eIF2 α , and ATF4. The quantitative densitometric analysis of Sec61γ (B), BiP (C), p-PERK/ PERK (D), p-eIF2 α /eIF2 α (E), and ATF4 (F) was carried out. A representative Western blot is shown (n = 3). *P < 0.05 vs 0 h treatment time point.

Application: WB    Species: human    Sample: MDA-MB-231 cells

Fig. 5.| DHT activated ER stress and UPR signaling pathways. MDA-MB-231 cells were incubated with 3 μM DHT for the indicated time. (A) Western blot analysis was performed with antibodies against Sec61γ, BiP, p-PERK/PERK, p-eIF2α/eIF2α, and ATF4. The quantitative densitometric analysis of Sec61γ (B), BiP (C), p-PERK/PERK (D), p-eIF2α/eIF2α (E), and ATF4 (F) was carried out.
2). SEC61G overexpression and DNA amplification correlates with prognosis and immune cell infiltration in head and neck squamous cell carcinoma. Cancer Medicine, 2021 (PubMed: 34590792) [IF=4.0]

Application: IHC    Species: Human    Sample: oropharyngeal cancer

FIGURE 6 The expression and prognostic values of SEC61G in oropharyngeal cancer: (A, B) representative IHC staining intensities of SEC61G in normal tissues: SEC61G was scored as (A) negative and (B) weak; (C–F) representative IHC staining patterns of SEC61G in OPC tissues: (C) negative, (D) weak, (E) moderate and (F) strong; (G, H) the proportions of SEC61G in normal and tumor tissues; (I) Overall survival rate of SEC61G in oropharyngeal cancer; (J) Progression‐free survival of SEC61G in oropharyngeal cancer

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