产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF12142, RRID:AB_2844947.
展开/折叠
110 kDa cell membrane glycoprotein; adhesion regulating molecule 1; Adhesion-regulating molecule 1; ADRM 1; Adrm1; ADRM1_HUMAN; ARM 1; ARM-1; ARM1; Gp110; hRpn13; M(r) 110,000 surface antigen; Proteasomal ubiquitin receptor ADRM1; proteasome regulatory particle non ATPase 13; Proteasome regulatory particle non-ATPase 13; Regulatory particle non ATPase 13; Rpn13; Rpn13 homolog;
抗原和靶标
- Q16186 ADRM1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MTTSGALFPSLVPGSRGASNKYLVEFRAGKMSLKGTTVTPDKRKGLVYIQQTDDSLIHFCWKDRTSGNVEDDLIIFPDDCEFKRVPQCPSGRVYVLKFKAGSKRLFFWMQEPKTDQDEEHCRKVNEYLNNPPMPGALGASGSSGHELSALGGEGGLQSLLGNMSHSQLMQLIGPAGLGGLGGLGALTGPGLASLLGSSGPPGSSSSSSSRSQSAAVTPSSTTSSTRATPAPSAPAAASATSPSPAPSSGNGASTAASPTQPIQLSDLQSILATMNVPAGPAGGQQVDLASVLTPEIMAPILANADVQERLLPYLPSGESLPQTADEIQNTLTSPQFQQALGMFSAALASGQLGPLMCQFGLPAEAVEAANKGDVEAFAKAMQNNAKPEQKEGDTKDKKDEEEDMSLD
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q16186 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T2 | Acetylation | Uniprot | |
| T2 | Phosphorylation | Uniprot | |
| T3 | Phosphorylation | Uniprot | |
| S4 | Phosphorylation | Uniprot | |
| S10 | Phosphorylation | Uniprot | |
| S15 | Phosphorylation | Uniprot | |
| S19 | Phosphorylation | Uniprot | |
| K21 | Acetylation | Uniprot | |
| K21 | Ubiquitination | Uniprot | |
| Y22 | Phosphorylation | Uniprot | |
| R27 | Methylation | Uniprot | |
| S32 | Phosphorylation | Uniprot | |
| K34 | Methylation | Uniprot | |
| K34 | Ubiquitination | Uniprot | |
| T37 | Phosphorylation | Uniprot | |
| T39 | Phosphorylation | Uniprot | |
| K42 | Methylation | Uniprot | |
| K42 | Ubiquitination | Uniprot | |
| K62 | Ubiquitination | Uniprot | |
| K83 | Acetylation | Uniprot | |
| K83 | Ubiquitination | Uniprot | |
| K97 | Ubiquitination | Uniprot | |
| K113 | Ubiquitination | Uniprot | |
| Y127 | Phosphorylation | Uniprot | |
| S140 | Phosphorylation | Uniprot | |
| S142 | Phosphorylation | Uniprot | |
| S143 | Phosphorylation | Uniprot | |
| S211 | Phosphorylation | Uniprot | |
| S213 | Phosphorylation | Uniprot | |
| T217 | Phosphorylation | Uniprot | |
| S220 | Phosphorylation | Uniprot | |
| T221 | Phosphorylation | Uniprot | |
| T222 | Phosphorylation | Uniprot | |
| S224 | Phosphorylation | Uniprot | |
| K371 | Ubiquitination | Uniprot | |
| K379 | Ubiquitination | Uniprot | |
| K386 | Ubiquitination | Uniprot | |
| K390 | Ubiquitination | Uniprot | |
| K395 | Ubiquitination | Uniprot | |
| K397 | Ubiquitination | Uniprot | |
| K398 | Ubiquitination | Uniprot | |
| S405 | Phosphorylation | Uniprot |
研究背景
Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. Within the complex, functions as a proteasomal ubiquitin receptor. Engages and activates 19S-associated deubiquitinases UCHL5 and PSMD14 during protein degradation. UCHL5 reversibly associate with the 19S regulatory particle whereas PSMD14 is an intrinsic subunit of the proteasome lid subcomplex.
Cytoplasm. Nucleus.
Component of the 19S proteasome regulatory particle complex. The 26S proteasome consists of a 20S core particle (CP) and two 19S regulatory subunits (RP). Interacts with the proteasomal scaffolding protein PSMD1. Interacts with deubiquitinase UCHL5; this interaction activates the auto-inhibited UCHL5 by deoligomerizing it. Interacts with UBQLN2 and ubiquitin.
The Pru (pleckstrin-like receptor for ubiquitin) domain mediates interactions with PSMD1 and ubiquitin. Preferential binding to the proximal subunit of K48-linked diubiquitin allows UCHL5 access to the distal subunit.
Belongs to the ADRM1 family.
研究领域
· Genetic Information Processing > Folding, sorting and degradation > Proteasome.
· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.
限制条款
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