| 产品: | ATP5F1 抗体 |
| 货号: | DF12144 |
| 描述: | Rabbit polyclonal antibody to ATP5F1 |
| 应用: | WB IHC |
| 反应: | Human, Mouse, Rat |
| 预测: | Bovine, Chicken |
| 分子量: | 25 kDa; 29kD(Calculated). |
| 蛋白号: | P24539 |
| RRID: | AB_2844949 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF12144, RRID:AB_2844949.
展开/折叠
AT5F1_HUMAN; ATP synthase B chain mitochondrial; ATP synthase subunit b; ATP synthase subunit b mitochondrial; ATP synthase, H+ transporting, mitochondrial F0 complex, subunit b,; ATP synthase, H+ transporting, mitochondrial F0 complex, subunit b, isoform 1; ATP synthase, H+ transporting, mitochondrial F0 complex, subunit B1; ATP5F1; ATPase subunit b; Cell proliferation inducing protein 47; H+ ATP synthase subunit b; MGC24431; mitochondrial; PIG47;
抗原和靶标
- P24539 AT5F1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MLSRVVLSAAATAAPSLKNAAFLGPGVLQATRTFHTGQPHLVPVPPLPEYGGKVRYGLIPEEFFQFLYPKTGVTGPYVLGTGLILYALSKEIYVISAETFTALSVLGVMVYGIKKYGPFVADFADKLNEQKLAQLEEAKQASIQHIQNAIDTEKSQQALVQKRHYLFDVQRNNIAMALEVTYRERLYRVYKEVKNRLDYHISVQNMMRRKEQEHMINWVEKHVVQSISTQQEKETIAKCIADLKLLAKKAQAQPVM
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P24539 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T31 | Phosphorylation | Uniprot | |
| K53 | Acetylation | Uniprot | |
| Y116 | Phosphorylation | Uniprot | |
| K126 | Ubiquitination | Uniprot | |
| K131 | Acetylation | Uniprot | |
| K131 | Ubiquitination | Uniprot | |
| K139 | Ubiquitination | Uniprot | |
| S142 | Phosphorylation | Uniprot | |
| T152 | Phosphorylation | Uniprot | |
| K154 | Acetylation | Uniprot | |
| K154 | Ubiquitination | Uniprot | |
| K162 | Acetylation | Uniprot | |
| K162 | Ubiquitination | Uniprot | |
| Y165 | Phosphorylation | Uniprot | |
| Y199 | Phosphorylation | Uniprot | |
| K221 | Acetylation | Uniprot | |
| K221 | Ubiquitination | Uniprot | |
| S226 | Phosphorylation | Uniprot | |
| T229 | Phosphorylation | Uniprot | |
| K233 | Acetylation | Uniprot | |
| K233 | Ubiquitination | Uniprot | |
| K238 | Acetylation | Uniprot | |
| K238 | Ubiquitination | Uniprot | |
| K244 | Acetylation | Uniprot | |
| K244 | Ubiquitination | Uniprot | |
| K248 | Acetylation | Uniprot |
研究背景
Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements.
Mitochondrion. Mitochondrion inner membrane.
F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three main subunits: a, b and c. Component of an ATP synthase complex composed of ATP5PB, ATP5MC1, ATP5F1E, ATP5PD, ATP5ME, ATP5PF, ATP5MF, MT-ATP6, MT-ATP8, ATP5F1A, ATP5F1B, ATP5F1D, ATP5F1C, ATP5PO, ATP5MG, ATP5MD and ATP5MPL (By similarity).
Belongs to the eukaryotic ATPase B chain family.
研究领域
· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.
· Human Diseases > Neurodegenerative diseases > Parkinson's disease.
· Human Diseases > Neurodegenerative diseases > Huntington's disease.
· Metabolism > Energy metabolism > Oxidative phosphorylation.
· Metabolism > Global and overview maps > Metabolic pathways.
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