产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF12155, RRID:AB_2844960.
展开/折叠
FLOT 1; FLOT1; FLOT1_HUMAN; Flotillin-1; Flotillin1; Integral membrane component of caveolae; Reggie 2;
抗原和靶标
- O75955 FLOT1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MFFTCGPNEAMVVSGFCRSPPVMVAGGRVFVLPCIQQIQRISLNTLTLNVKSEKVYTRHGVPISVTGIAQVKIQGQNKEMLAAACQMFLGKTEAEIAHIALETLEGHQRAIMAHMTVEEIYKDRQKFSEQVFKVASSDLVNMGISVVSYTLKDIHDDQDYLHSLGKARTAQVQKDARIGEAEAKRDAGIREAKAKQEKVSAQYLSEIEMAKAQRDYELKKAAYDIEVNTRRAQADLAYQLQVAKTKQQIEEQRVQVQVVERAQQVAVQEQEIARREKELEARVRKPAEAERYKLERLAEAEKSQLIMQAEAEAASVRMRGEAEAFAIGARARAEAEQMAKKAEAFQLYQEAAQLDMLLEKLPQVAEEISGPLTSANKITLVSSGSGTMGAAKVTGEVLDILTRLPESVERLTGVSISQVNHKPLRTA
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O75955 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S19 | Phosphorylation | Uniprot | |
| K51 | Ubiquitination | Uniprot | |
| K54 | Ubiquitination | Uniprot | |
| Y56 | Phosphorylation | P12931 (SRC) | Uniprot |
| K78 | Ubiquitination | Uniprot | |
| K122 | Ubiquitination | Uniprot | |
| K126 | Ubiquitination | Uniprot | |
| S128 | Phosphorylation | Uniprot | |
| Y149 | Phosphorylation | P12931 (SRC) | Uniprot |
| K152 | Ubiquitination | Uniprot | |
| Y160 | Phosphorylation | P06241 (FYN) | Uniprot |
| S163 | Phosphorylation | Uniprot | |
| K166 | Ubiquitination | Uniprot | |
| K174 | Ubiquitination | Uniprot | |
| K184 | Ubiquitination | Uniprot | |
| K195 | Ubiquitination | Uniprot | |
| K198 | Ubiquitination | Uniprot | |
| Y203 | Phosphorylation | Uniprot | |
| S205 | Phosphorylation | Uniprot | |
| K211 | Ubiquitination | Uniprot | |
| Y216 | Phosphorylation | Uniprot | |
| K220 | Methylation | Uniprot | |
| K220 | Ubiquitination | Uniprot | |
| Y223 | Phosphorylation | Uniprot | |
| Y238 | Phosphorylation | Uniprot | |
| K244 | Ubiquitination | Uniprot | |
| Y292 | Phosphorylation | Uniprot | |
| K293 | Ubiquitination | Uniprot | |
| K302 | Ubiquitination | Uniprot | |
| S315 | Phosphorylation | Uniprot | |
| K341 | Ubiquitination | Uniprot | |
| Y348 | Phosphorylation | Uniprot | |
| S383 | Phosphorylation | Uniprot | |
| S385 | Phosphorylation | Uniprot | |
| T387 | Phosphorylation | Uniprot | |
| K392 | Ubiquitination | Uniprot | |
| S407 | Phosphorylation | Uniprot | |
| K422 | Ubiquitination | Uniprot |
研究背景
May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles.
Cell membrane>Peripheral membrane protein. Endosome. Membrane>Caveola>Peripheral membrane protein. Melanosome. Membrane raft.
Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:17081065). Membrane-associated protein of caveola (By similarity).
Heterooligomeric complex of flotillin-1 and flotillin-2 and caveolin-1 and caveolin-2 (By similarity). Interacts with ECPAS.
Belongs to the band 7/mec-2 family. Flotillin subfamily.
研究领域
· Organismal Systems > Endocrine system > Insulin signaling pathway. (View pathway)
限制条款
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