MYH4 Antibody - #DF12165

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产品: MYH4 抗体
货号: DF12165
描述: Rabbit polyclonal antibody to MYH4
应用: WB IHC
反应: Human, Mouse
预测: Pig, Bovine, Sheep, Rabbit, Dog
分子量: 230kd; 223kD(Calculated).
蛋白号: Q9Y623
RRID: AB_2844970

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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相关下载
MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse
预测:
Pig(100%), Bovine(100%), Sheep(100%), Rabbit(92%), Dog(92%)
克隆:
Polyclonal
特异性:
MYH4 Antibody detects endogenous levels of total MYH4.
RRID:
AB_2844970
引用格式: Affinity Biosciences Cat# DF12165, RRID:AB_2844970.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

fetal; MYH2B; Myh4; MYH4_HUMAN; MyHC-2b; MyHC-IIb; Myosin heavy chain 2b; Myosin heavy chain 4; Myosin heavy chain 4 skeletal muscle; Myosin heavy chain; Myosin heavy chain IIb; Myosin heavy chain skeletal muscle fetal; Myosin-4; skeletal muscle;

抗原和靶标

免疫原:
Uniprot:

Q9Y623(MYH4_HUMAN) >>Visit HPA database.

基因/基因ID:
MYH4(4622)
序列:
MSSDSEMAIFGEAAPFLRKSEKERIEAQNKPFDAKTSVFVVDPKESYVKAIVQSREGGKVTAKTEAGATVTVKEDQVFSMNPPKYDKIEDMAMMTHLHEPAVLYNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYNPEVVTAYRGKKRQEAPPHIFSISDNAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAVTGEKKKEEPASGKMQGTLEDQIISANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADIETYLLEKSRVTFQLKAERSYHIFYQILSNKKPELIEMLLITTNPYDFAFVSQGEITVPSIDDQEELMATDSAVDILGFTADEKVAIYKLTGAVMHYGNMKFKQKQREEQAEPDGTEVADKAAYLTSLNSADLLKSLCYPRVKVGNEFVTKGQTVQQVYNAVGALAKAIYEKMFLWMVTRINQQLDTKQPRQYFIGVLDIAGFEIFDFNSLEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWEFIDFGMDLAACIELIEKPMGIFSILEEECMFPKATDTSFKNKLYEQHLGKSNNFQKPKPAKGKPEAHFSLVHYAGTVDYNIAGWLDKNKDPLNETVVGLYQKSAMKTLAFLFSGAQTAEAEGGGGKKGGKKKGSSFQTVSALFRENLNKLMTNLRSTHPHFVRCIIPNETKTPGAMEHELVLHQLRCNGVLEGIRICRKGFPSRILYADFKQRYKVLNASAIPEGQFIDSKKASEKLLGSIEIDHTQYKFGHTKVFFKAGLLGTLEEMRDEKLAQLITRTQAICRGFLMRVEFRKMMERRESIFCIQYNIRAFMNVKHWPWMKLYFKIKPLLKSAETEKEMANMKEEFEKTKEELAKTEAKRKELEEKMVTLMQEKNDLQLQVQAEADALADAEERCDQLIKTKIQLEAKIKEVTERAEDEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLTEEMAGLDETIAKLTKEKKALQEAHQQTLDDLQMEEDKVNTLTKAKTKLEQQVDDLEGSLEQEKKLCMDLERAKRKLEGDLKLAQESTMDTENDKQQLNEKLKKKEFEMSNLQGKIEDEQALAIQLQKKIKELQARIEELEEEIEAERASRAKAEKQRSDLSRELEEISERLEEAGGATSAQIEMNKKREAEFQKMRRDLEESTLQHEATAAALRKKHADSVAELGEQIDSLQRVKQKLEKEKSELKMEINDLASNMETVSKAKANFEKMCRTLEDQLSEIKTKEEEQQRLINELSAQKARLHTESGEFSRQLDEKDAMVSQLSRGKQAFTQQIEELKRQLEEETKAKSTLAHALQSARHDCDLLREQYEEEQEAKAELQRGMSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQDAEEHVEAVNSKCASLEKTKQRLQNEVEDLMIDVERSNAACIALDKKQRNFDKVLAEWKQKYEETQAELEASQKESRSLSTELFKVKNAYEESLDHLETLKRENKNLQQEISDLTEQIAEGGKHIHELEKVKKQLDHEKSELQTSLEEAEASLEHEEGKILRIQLELNQVKSEIDRKIAEKDEELDQLKRNHLRVVESMQSTLDAEIRSRNDALRIKKKMEGDLNEMEIQLNHANRQAAEALRNLRNTQGILKDTQLHLDDAIRGQDDLKEQLAMVERRANLMQAEVEELRASLERTERGRKMAEQELLDASERVQLLHTQNTSLINTKKKLETDISQIQGEMEDIVQEARNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNMEQTVKDLQLRLDEAEQLALKGGKKQIQKLEARVRELESEVESEQKHNVEAVKGLRKHERRVKELTYQTEEDRKNILRLQDLVDKLQTKVKAYKRQAEEAEEQSNVNLAKFRKLQHELEEAKERADIAESQVNKLRVKSREVHTKVISEE

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
92
Rabbit
92
Horse
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q9Y623 作为底物

Site PTM Type Enzyme
T64 Phosphorylation
T69 Phosphorylation
Y163 Phosphorylation
T168 Phosphorylation
Y195 Phosphorylation
T198 Phosphorylation
K249 Ubiquitination
T258 Phosphorylation
S263 Phosphorylation
Y269 Phosphorylation
K273 Ubiquitination
T356 Phosphorylation
Y362 Phosphorylation
T381 Phosphorylation
Y389 Phosphorylation
Y424 Phosphorylation
K505 Acetylation
Y556 Phosphorylation
T607 Phosphorylation
Y612 Phosphorylation
T664 Phosphorylation
Y719 Phosphorylation
S732 Phosphorylation
S742 Phosphorylation
K744 Acetylation
S746 Phosphorylation
T790 Phosphorylation
Y820 Phosphorylation
K857 Methylation
K862 Methylation
K922 Acetylation
S952 Phosphorylation
K955 Acetylation
K956 Acetylation
T964 Phosphorylation
T983 Phosphorylation
T992 Phosphorylation
T997 Phosphorylation
K1030 Methylation
S1041 Phosphorylation
K1046 Acetylation
K1087 Methylation
K1097 Methylation
K1111 Acetylation
K1113 Acetylation
R1130 Methylation
S1132 Phosphorylation
S1141 Phosphorylation
S1144 Phosphorylation
T1161 Phosphorylation
S1162 Phosphorylation
S1185 Phosphorylation
T1186 Phosphorylation
T1192 Phosphorylation
K1223 Ubiquitination
K1251 Ubiquitination
S1261 Phosphorylation
K1264 Ubiquitination
T1265 Phosphorylation
S1278 Phosphorylation
T1286 Phosphorylation
S1288 Phosphorylation
S1292 Phosphorylation
S1303 Phosphorylation
S1306 Phosphorylation
T1313 Phosphorylation
Y1351 Phosphorylation
S1366 Phosphorylation
S1370 Phosphorylation
T1377 Phosphorylation
Y1379 Phosphorylation
T1381 Phosphorylation
T1387 Phosphorylation
K1394 Acetylation
K1395 Acetylation
K1396 Acetylation
S1413 Phosphorylation
T1421 Phosphorylation
Y1464 Phosphorylation
T1467 Phosphorylation
S1478 Phosphorylation
S1480 Phosphorylation
S1482 Phosphorylation
T1483 Phosphorylation
S1514 Phosphorylation
T1517 Phosphorylation
S1574 Phosphorylation
S1600 Phosphorylation
S1603 Phosphorylation
T1604 Phosphorylation
S1714 Phosphorylation
T1722 Phosphorylation
T1725 Phosphorylation
S1726 Phosphorylation
T1730 Phosphorylation
K1731 Acetylation
S1739 Phosphorylation
T1764 Phosphorylation
K1774 Acetylation
K1788 Acetylation
K1795 Acetylation
K1813 Ubiquitination
K1814 Ubiquitination
T1855 Phosphorylation
Y1856 Phosphorylation
K1863 Acetylation
S1919 Phosphorylation

研究背景

功能:

Muscle contraction.

细胞定位:

Cytoplasm>Myofibril.
Note: Thick filaments of the myofibrils.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2).

蛋白家族:

The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.

Limited proteolysis of myosin heavy chain produces 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). HMM can be further cleaved into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.

研究领域

· Cellular Processes > Cellular community - eukaryotes > Tight junction.   (View pathway)

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