产品: | YTHDF2 抗体 |
货号: | DF12182 |
描述: | Rabbit polyclonal antibody to YTHDF2 |
应用: | WB IHC |
反应: | Human, Mouse, Rat |
预测: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken |
分子量: | 62 kDa; 62kD(Calculated). |
蛋白号: | Q9Y5A9 |
RRID: | AB_2844987 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF12182, RRID:AB_2844987.
展开/折叠
9430020E02Rik; CAHL; CLL associated antigen KW 14; CLL-associated antigen KW-14; HGRG8; High glucose regulated protein 8; High-glucose-regulated protein 8; NY REN 2; Renal carcinoma antigen NY REN 2; Renal carcinoma antigen NY-REN-2; YTH domain family 2; YTH domain family member 2; YTH domain family protein 2; YTH N6 methyladenosine RNA binding protein 2; YTHD2_HUMAN; YTHDF 2; YTHDF2;
抗原和靶标
- Q9Y5A9 YTHD2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSASSLLEQRPKGQGNKVQNGSVHQKDGLNDDDFEPYLSPQARPNNAYTAMSDSYLPSYYSPSIGFSYSLGEAAWSTGGDTAMPYLTSYGQLSNGEPHFLPDAMFGQPGALGSTPFLGQHGFNFFPSGIDFSAWGNNSSQGQSTQSSGYSSNYAYAPSSLGGAMIDGQSAFANETLNKAPGMNTIDQGMAALKLGSTEVASNVPKVVGSAVGSGSITSNIVASNSLPPATIAPPKPASWADIASKPAKQQPKLKTKNGIAGSSLPPPPIKHNMDIGTWDNKGPVAKAPSQALVQNIGQPTQGSPQPVGQQANNSPPVAQASVGQQTQPLPPPPPQPAQLSVQQQAAQPTRWVAPRNRGSGFGHNGVDGNGVGQSQAGSGSTPSEPHPVLEKLRSINNYNPKDFDWNLKHGRVFIIKSYSEDDIHRSIKYNIWCSTEHGNKRLDAAYRSMNGKGPVYLLFSVNGSGHFCGVAEMKSAVDYNTCAGVWSQDKWKGRFDVRWIFVKDVPNSQLRHIRLENNENKPVTNSRDTQEVPLEKAKQVLKIIASYKHTTSIFDDFSHYEKRQEEEESVKKERQGRGK
种属预测
score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9Y5A9 作为底物
Site | PTM Type | Enzyme | Source |
---|---|---|---|
S2 | Acetylation | Uniprot | |
S2 | Phosphorylation | Uniprot | |
S4 | Phosphorylation | Uniprot | |
S5 | Phosphorylation | Uniprot | |
K12 | Ubiquitination | Uniprot | |
K17 | Ubiquitination | Uniprot | |
K26 | Ubiquitination | Uniprot | |
Y37 | Phosphorylation | Uniprot | |
S39 | Phosphorylation | Uniprot | |
K193 | Ubiquitination | Uniprot | |
S196 | Phosphorylation | Uniprot | |
S238 | Phosphorylation | Uniprot | |
K245 | Acetylation | Uniprot | |
T277 | Phosphorylation | Uniprot | |
K281 | Ubiquitination | Uniprot | |
R350 | Methylation | Uniprot | |
R357 | Methylation | Uniprot | |
S359 | Phosphorylation | Uniprot | |
S380 | Phosphorylation | Uniprot | |
T381 | Phosphorylation | Uniprot | |
S383 | Phosphorylation | Uniprot | |
K391 | Ubiquitination | Uniprot | |
R393 | Methylation | Uniprot | |
S394 | Phosphorylation | Uniprot | |
K401 | Acetylation | Uniprot | |
K401 | Ubiquitination | Uniprot | |
K408 | Ubiquitination | Uniprot | |
K416 | Ubiquitination | Uniprot | |
S419 | Phosphorylation | Uniprot | |
K428 | Ubiquitination | Uniprot | |
Y446 | Phosphorylation | Uniprot | |
K490 | Ubiquitination | Uniprot | |
K521 | Ubiquitination | Uniprot | |
K536 | Ubiquitination | Uniprot | |
K548 | Ubiquitination | Uniprot | |
S558 | Phosphorylation | Uniprot | |
Y560 | Phosphorylation | Uniprot | |
K562 | Ubiquitination | Uniprot | |
S569 | Phosphorylation | Uniprot |
研究背景
Specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs, and regulates mRNA stability. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in mRNA stability and processing. Acts as a regulator of mRNA stability by promoting degradation of m6A-containing mRNAs via interaction with the CCR4-NOT and ribonuclease P/MRP complexes, depending on the context. M6A-containing mRNAs containing a binding site for RIDA/HRSP12 (5'-GGUUC-3') are preferentially degraded by endoribonucleolytic cleavage: cooperative binding of RIDA/HRSP12 and YTHDF2 to transcripts leads to recruitment of the ribonuclease P/MRP complex. Other m6A-containing mRNAs undergo deadenylation via direct interaction between YTHDF2 and CNOT1, leading to recruitment of the CCR4-NOT and subsequent deadenylation of m6A-containing mRNAs. Required maternally to regulate oocyte maturation: probably acts by binding to m6A-containing mRNAs, thereby regulating maternal transcript dosage during oocyte maturation, which is essential for the competence of oocytes to sustain early zygotic development (By similarity). Also involved in hematopoietic stem cells specification by binding to m6A-containing mRNAs, leading to promote their degradation. Also acts as a regulator of neural development by promoting m6A-dependent degradation of neural development-related mRNA targets (By similarity). Regulates circadian regulation of hepatic lipid metabolism: acts by promoting m6A-dependent degradation of PPARA transcripts. Regulates the innate immune response to infection by inhibiting the type I interferon response: acts by binding to m6A-containing IFNB transcripts and promoting their degradation. Also acts as a promoter of cap-independent mRNA translation following heat shock stress: upon stress, relocalizes to the nucleus and specifically binds mRNAs with some m6A methylation mark at their 5'-UTR, protecting demethylation of mRNAs by FTO, thereby promoting cap-independent mRNA translation.
(Microbial infection) Promotes viral gene expression and replication of polyomavirus SV40: acts by binding to N6-methyladenosine (m6A)-containing viral RNAs.
(Microbial infection) Promotes viral gene expression and virion production of kaposis sarcoma-associated herpesvirus (KSHV) at some stage of the KSHV life cycle (in iSLK.219 and iSLK.BAC16 cells). Acts by binding to N6-methyladenosine (m6A)-containing viral RNAs.
Cytoplasm>Cytosol. Nucleus. Cytoplasm>P-body.
Note: Localizes to the cytosol and relocates to the nucleus following heat shock stress (PubMed:26458103).
Interacts with YTHDF3. Interacts with CNOT1; interaction is direct and promotes recruitment of the CCR4-NOT complex. Interacts with RIDA/HRSP12; interaction leads to recruitment of the ribonuclease P/MRP complex.
Belongs to the YTHDF2 family.
限制条款
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