产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF12213, RRID:AB_2845018.
展开/折叠
Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial; Descriptions; Methenyltetrahydrofolate cyclohydrolase; methylene tetrahydrofolate dehydrogenase (NAD+ dependent); methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2; methylenetetrahydrofolate dehydrogenase (NADP+ dependent); methylenetetrahydrofolate dehydrogenase 2; MGC82516; MTDC_HUMAN; MTHFD2; mthfd2 methylene tetrahydrofolate dehydrogenase (NAD+ dependent); NAD-dependent methylene tetrahydrofolate dehydrogenase; NAD-dependent methylenetetrahydrofolate dehydrogenase; NMDMC;
抗原和靶标
- P13995 MTDC_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAATSLMSALAARLLQPAHSCSLRLRPFHLAAVRNEAVVISGRKLAQQIKQEVRQEVEEWVASGNKRPHLSVILVGENPASHSYVLNKTRAAAVVGINSETIMKPASISEEELLNLINKLNNDDNVDGLLVQLPLPEHIDERRICNAVSPDKDVDGFHVINVGRMCLDQYSMLPATPWGVWEIIKRTGIPTLGKNVVVAGRSKNVGMPIAMLLHTDGAHERPGGDATVTISHRYTPKEQLKKHTILADIVISAAGIPNLITADMIKEGAAVIDVGINRVHDPVTAKPKLVGDVDFEGVRQKAGYITPVPGGVGPMTVAMLMKNTIIAAKKVLRLEEREVLKSKELGVATN
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P13995 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K50 | Acetylation | Uniprot | |
| K50 | Ubiquitination | Uniprot | |
| K88 | Ubiquitination | Uniprot | |
| K119 | Ubiquitination | Uniprot | |
| R143 | Methylation | Uniprot | |
| S149 | Phosphorylation | Uniprot | |
| K152 | Acetylation | Uniprot | |
| K152 | Ubiquitination | Uniprot | |
| T187 | Phosphorylation | Uniprot | |
| T191 | Phosphorylation | Uniprot | |
| K194 | Ubiquitination | Uniprot | |
| K237 | Ubiquitination | Uniprot | |
| K286 | Ubiquitination | Uniprot | |
| K288 | Ubiquitination | Uniprot | |
| T306 | Phosphorylation | Uniprot | |
| T324 | Phosphorylation | Uniprot | |
| K329 | Acetylation | Uniprot | |
| K343 | Ubiquitination | Uniprot |
研究背景
Although its dehydrogenase activity is NAD-specific, it can also utilize NADP at a reduced efficiency.
Mitochondrion.
Homodimer.
Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.
研究领域
· Metabolism > Metabolism of cofactors and vitamins > One carbon pool by folate.
· Metabolism > Global and overview maps > Metabolic pathways.
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