产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF12219, RRID:AB_2845024.
展开/折叠
2-oxoglutarate-4-dioxygenase subunit alpha-1; 4-hydroxylase, alpha I subunit; 4-PH alpha-1; P4HA; P4HA1; P4HA1_HUMAN; Procollagen-proline; Procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide I; Prolyl 4-hydroxylase subunit alpha-1;
抗原和靶标
- P13674 P4HA1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MIWYILIIGILLPQSLAHPGFFTSIGQMTDLIHTEKDLVTSLKDYIKAEEDKLEQIKKWAEKLDRLTSTATKDPEGFVGHPVNAFKLMKRLNTEWSELENLVLKDMSDGFISNLTIQRQYFPNDEDQVGAAKALLRLQDTYNLDTDTISKGNLPGVKHKSFLTAEDCFELGKVAYTEADYYHTELWMEQALRQLDEGEISTIDKVSVLDYLSYAVYQQGDLDKALLLTKKLLELDPEHQRANGNLKYFEYIMAKEKDVNKSASDDQSDQKTTPKKKGVAVDYLPERQKYEMLCRGEGIKMTPRRQKKLFCRYHDGNRNPKFILAPAKQEDEWDKPRIIRFHDIISDAEIEIVKDLAKPRLRRATISNPITGDLETVHYRISKSAWLSGYENPVVSRINMRIQDLTGLDVSTAEELQVANYGVGGQYEPHFDFARKDEPDAFKELGTGNRIATWLFYMSDVSAGGATVFPEVGASVWPKKGTAVFWYNLFASGEGDYSTRHAACPVLVGNKWVSNKWLHERGQEFRRPCTLSELE
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P13674 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T40 | Phosphorylation | Uniprot | |
| S41 | Phosphorylation | Uniprot | |
| K43 | Ubiquitination | Uniprot | |
| Y45 | Phosphorylation | Uniprot | |
| K47 | Ubiquitination | Uniprot | |
| K62 | Ubiquitination | Uniprot | |
| K72 | Ubiquitination | Uniprot | |
| N113 | N-Glycosylation | Uniprot | |
| K132 | Ubiquitination | Uniprot | |
| K150 | Ubiquitination | Uniprot | |
| K230 | Ubiquitination | Uniprot | |
| Y282 | Phosphorylation | Uniprot | |
| K288 | Ubiquitination | Uniprot | |
| K299 | Ubiquitination | Uniprot | |
| K327 | Ubiquitination | Uniprot | |
| S345 | Phosphorylation | Uniprot | |
| K353 | Ubiquitination | Uniprot | |
| S366 | Phosphorylation | Uniprot | |
| K382 | Acetylation | Uniprot | |
| K382 | Ubiquitination | Uniprot | |
| S387 | Phosphorylation | Uniprot | |
| K442 | Ubiquitination | Uniprot | |
| K510 | Ubiquitination | Uniprot | |
| K515 | Ubiquitination | Uniprot |
研究背景
Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins.
Endoplasmic reticulum lumen.
Heterotetramer of two alpha-1 chains and two beta chains (the beta chain is the multi-functional PDI).
Belongs to the P4HA family.
研究领域
· Metabolism > Amino acid metabolism > Arginine and proline metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
限制条款
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