产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF12229, RRID:AB_2845034.
展开/折叠
AF17q25; Cell division control protein septin D1; KIAA0991; MLL septin like fusion protein; MLL septin-like fusion protein; MLL septin-like fusion protein MSF-A; MSF; MSF1; NAPB; Ov/Br septin; Ovarian/breast septin alpha; Ovarian/Breast septin; PNUTL4; SEPT9; SEPT9_HUMAN; SeptD1; Septin 9; Septin D1; Septin-9; SINT1;
抗原和靶标
Widely expressed. Isoforms are differentially expressed in testes, kidney, liver heart, spleen, brain, peripheral blood leukocytes, skeletal muscle and kidney. Specific isoforms appear to demonstrate tissue specificity. Isoform 5 is the most highly expressed in fetal tissue. Isoform 1 is detected in all tissues except the brain and thymus, while isoform 2, isoform 3, and isoform 4 are detected at low levels in approximately half of the fetal tissues.
- Q9UHD8 SEPT9_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MKKSYSGGTRTSSGRLRRLGDSSGPALKRSFEVEEVETPNSTPPRRVQTPLLRATVASSTQKFQDLGVKNSEPSARHVDSLSQRSPKASLRRVELSGPKAAEPVSRRTELSIDISSKQVENAGAIGPSRFGLKRAEVLGHKTPEPAPRRTEITIVKPQESAHRRMEPPASKVPEVPTAPATDAAPKRVEIQMPKPAEAPTAPSPAQTLENSEPAPVSQLQSRLEPKPQPPVAEATPRSQEATEAAPSCVGDMADTPRDAGLKQAPASRNEKAPVDFGYVGIDSILEQMRRKAMKQGFEFNIMVVGQSGLGKSTLINTLFKSKISRKSVQPTSEERIPKTIEIKSITHDIEEKGVRMKLTVIDTPGFGDHINNENCWQPIMKFINDQYEKYLQEEVNINRKKRIPDTRVHCCLYFIPATGHSLRPLDIEFMKRLSKVVNIVPVIAKADTLTLEERVHFKQRITADLLSNGIDVYPQKEFDEDSEDRLVNEKFREMIPFAVVGSDHEYQVNGKRILGRKTKWGTIEVENTTHCEFAYLRDLLIRTHMQNIKDITSSIHFEAYRVKRLNEGSSAMANGMEEKEPEAPEM
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9UHD8 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| K2 | Methylation | Uniprot | |
| K3 | Methylation | Uniprot | |
| S6 | Phosphorylation | Uniprot | |
| R10 | Methylation | Uniprot | |
| T11 | Phosphorylation | Uniprot | |
| S12 | Phosphorylation | Uniprot | |
| S13 | Phosphorylation | Uniprot | |
| S22 | Phosphorylation | Uniprot | |
| K28 | Acetylation | Uniprot | |
| K28 | Methylation | Uniprot | |
| K28 | Ubiquitination | Uniprot | |
| S30 | Phosphorylation | Uniprot | |
| T38 | Phosphorylation | Uniprot | |
| S41 | Phosphorylation | Uniprot | |
| T42 | Phosphorylation | Uniprot | |
| T49 | Phosphorylation | Uniprot | |
| S59 | Phosphorylation | Uniprot | |
| T60 | Phosphorylation | Uniprot | |
| K62 | Acetylation | Uniprot | |
| K62 | Ubiquitination | Uniprot | |
| S71 | Phosphorylation | Uniprot | |
| S80 | Phosphorylation | Uniprot | |
| S82 | Phosphorylation | Uniprot | |
| S85 | Phosphorylation | Uniprot | |
| S89 | Phosphorylation | Uniprot | |
| S96 | Phosphorylation | Uniprot | |
| S111 | Phosphorylation | Uniprot | |
| S115 | Phosphorylation | Uniprot | |
| K141 | Ubiquitination | Uniprot | |
| T142 | Phosphorylation | Uniprot | |
| S160 | Phosphorylation | Uniprot | |
| K186 | Ubiquitination | Uniprot | |
| S203 | Phosphorylation | Uniprot | |
| S211 | Phosphorylation | Uniprot | |
| S217 | Phosphorylation | Uniprot | |
| T235 | Phosphorylation | Uniprot | |
| S238 | Phosphorylation | Uniprot | |
| T242 | Phosphorylation | Uniprot | |
| S247 | Phosphorylation | Uniprot | |
| C248 | S-Nitrosylation | Uniprot | |
| T255 | Phosphorylation | Uniprot | |
| K262 | Ubiquitination | Uniprot | |
| K271 | Ubiquitination | Uniprot | |
| Y278 | Phosphorylation | Uniprot | |
| S283 | Phosphorylation | Uniprot | |
| K291 | Ubiquitination | Uniprot | |
| T317 | Phosphorylation | Uniprot | |
| K320 | Methylation | Uniprot | |
| K320 | Ubiquitination | Uniprot | |
| K322 | Methylation | Uniprot | |
| K326 | Ubiquitination | Uniprot | |
| S327 | Phosphorylation | Uniprot | |
| T331 | Phosphorylation | Uniprot | |
| S332 | Phosphorylation | Uniprot | |
| K338 | Ubiquitination | Uniprot | |
| K343 | Ubiquitination | Uniprot | |
| K352 | Acetylation | Uniprot | |
| K352 | Ubiquitination | Uniprot | |
| K357 | Ubiquitination | Uniprot | |
| T363 | Phosphorylation | Uniprot | |
| K381 | Ubiquitination | Uniprot | |
| K389 | Ubiquitination | Uniprot | |
| K400 | Ubiquitination | Uniprot | |
| K431 | Acetylation | Uniprot | |
| K431 | Ubiquitination | Uniprot | |
| K435 | Ubiquitination | Uniprot | |
| K445 | Ubiquitination | Uniprot | |
| T448 | Phosphorylation | Uniprot | |
| K458 | Ubiquitination | Uniprot | |
| K476 | Acetylation | Uniprot | |
| K476 | Ubiquitination | Uniprot | |
| S482 | Phosphorylation | Uniprot | |
| R485 | Methylation | Uniprot | |
| K490 | Ubiquitination | Uniprot | |
| S502 | Phosphorylation | Uniprot | |
| Y506 | Phosphorylation | Uniprot | |
| K511 | Acetylation | Uniprot | |
| K511 | Ubiquitination | Uniprot | |
| K519 | Ubiquitination | Uniprot | |
| T543 | Phosphorylation | Uniprot | |
| K549 | Ubiquitination | Uniprot |
研究背景
Filament-forming cytoskeletal GTPase (By similarity). May play a role in cytokinesis (Potential). May play a role in the internalization of 2 intracellular microbial pathogens, Listeria monocytogenes and Shigella flexneri.
Cytoplasm>Cytoskeleton.
Note: In an epithelial cell line, concentrates at cell-cell contact areas. After TGF-beta1 treatment and induction of epithelial to mesenchymal transition, colocalizes partly with actin stress fibers. During bacterial infection, displays a collar shape structure next to actin at the pole of invading bacteria.
Widely expressed. Isoforms are differentially expressed in testes, kidney, liver heart, spleen, brain, peripheral blood leukocytes, skeletal muscle and kidney. Specific isoforms appear to demonstrate tissue specificity. Isoform 5 is the most highly expressed in fetal tissue. Isoform 1 is detected in all tissues except the brain and thymus, while isoform 2, isoform 3, and isoform 4 are detected at low levels in approximately half of the fetal tissues.
Septins polymerize into heterooligomeric protein complexes that form filaments, and associate with cellular membranes, actin filaments, and microtubules. GTPase activity is required for filament formation. Interacts with SEPTIN2, SEPTIN6, SEPTIN7, SEPTIN11 and SEPTIN14. Interacts with RTKN and ARHGEF18. In a mesenchymal cell line, Rho/RTKN signals cause disruption of wild-type septin filaments, but not of those containing isoform 2 variants HNA Trp-106 and Phe-111. In a mesenchymal cell line, isoform 2 variants HNA Trp-106 and Phe-111, but not wild type, form filaments with SEPTIN4.
Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Septin GTPase family.
研究领域
· Human Diseases > Infectious diseases: Bacterial > Bacterial invasion of epithelial cells.
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