产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF12257, RRID:AB_2845062.
展开/折叠
Acidic-type mitochondrial creatine kinase; CKMT; CKMT1; CKMT1B; Creatine kinase mitochondrial 1 (ubiquitous); Creatine kinase mitochondrial 1B; Creatine kinase U type mitochondrial; Creatine kinase U-type; KCRU_HUMAN; Mia-CK; mitochondrial; U-MtCK; Ubiquitous mitochondrial creatine kinase; UMTCK;
抗原和靶标
- P12532 KCRU_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAGPFSRLLSARPGLRLLALAGAGSLAAGFLLRPEPVRAASERRRLYPPSAEYPDLRKHNNCMASHLTPAVYARLCDKTTPTGWTLDQCIQTGVDNPGHPFIKTVGMVAGDEETYEVFADLFDPVIQERHNGYDPRTMKHTTDLDASKIRSGYFDERYVLSSRVRTGRSIRGLSLPPACTRAERREVERVVVDALSGLKGDLAGRYYRLSEMTEAEQQQLIDDHFLFDKPVSPLLTAAGMARDWPDARGIWHNNEKSFLIWVNEEDHTRVISMEKGGNMKRVFERFCRGLKEVERLIQERGWEFMWNERLGYILTCPSNLGTGLRAGVHIKLPLLSKDSRFPKILENLRLQKRGTGGVDTAATGGVFDISNLDRLGKSEVELVQLVIDGVNYLIDCERRLERGQDIRIPTPVIHTKH
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P12532 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| C62 | S-Nitrosylation | Uniprot | |
| T141 | Phosphorylation | Uniprot | |
| S147 | Phosphorylation | Uniprot | |
| S151 | Phosphorylation | Uniprot | |
| Y153 | Phosphorylation | Uniprot | |
| R157 | Methylation | Uniprot | |
| T166 | Phosphorylation | Uniprot | |
| S169 | Phosphorylation | Uniprot | |
| S196 | Phosphorylation | Uniprot | |
| S232 | Phosphorylation | Uniprot | |
| Y312 | Phosphorylation | Uniprot | |
| S318 | Phosphorylation | Uniprot | |
| S336 | Phosphorylation | Uniprot | |
| S339 | Phosphorylation | Uniprot | |
| R349 | Methylation | Uniprot | |
| R407 | Methylation | Uniprot | |
| T410 | Phosphorylation | Uniprot |
研究背景
Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
Mitochondrion inner membrane>Peripheral membrane protein>Intermembrane side.
Exists as an octamer composed of four MTCK homodimers.
Belongs to the ATP:guanido phosphotransferase family.
研究领域
· Metabolism > Amino acid metabolism > Arginine and proline metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
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