Mast Cell Chymase Antibody - #DF12290

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产品: Mast Cell Chymase 抗体
货号: DF12290
描述: Rabbit polyclonal antibody to Mast Cell Chymase
应用: WB IHC
文献验证:
反应: Human, Mouse, Rat
预测: Dog
分子量: 27-30 kDa; 27kD(Calculated).
蛋白号: P23946
RRID: AB_2845095

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 50ul RMB¥ 1250 现货
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MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Dog(83%)
克隆:
Polyclonal
特异性:
Mast Cell Chymase Antibody detects endogenous levels of total Mast Cell Chymase.
RRID:
AB_2845095
引用格式: Affinity Biosciences Cat# DF12290, RRID:AB_2845095.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Alpha-chymase; Chymase 1; Chymase 1 mast cell; chymase 1 preproprotein transcript E; chymase 1 preproprotein transcript I; Chymase; Chymase, heart; Chymase, mast cell; CMA1; CMA1_HUMAN; CYH; CYM; EC 3.4.21.39; Mast cell chymase 1; Mast cell protease 3; Mast cell protease 5; Mast cell protease I; Mast cell protease III; Mcp-5; MCP3P; Mcpt5; MCT1; MGC119890; MGC119891; MMCP-5;

抗原和靶标

免疫原:
Uniprot:

P23946(CMA1_HUMAN) >>Visit HPA database.

基因/基因ID:
CMA1(1215)
表达:
P23946 CMA1_HUMAN:

Mast cells in lung, heart, skin and placenta. Expressed in both normal skin and in urticaria pigmentosa lesions.

序列:
MLLLPLPLLLFLLCSRAEAGEIIGGTECKPHSRPYMAYLEIVTSNGPSKFCGGFLIRRNFVLTAAHCAGRSITVTLGAHNITEEEDTWQKLEVIKQFRHPKYNTSTLHHDIMLLKLKEKASLTLAVGTLPFPSQFNFVPPGRMCRVAGWGRTGVLKPGSDTLQEVKLRLMDPQACSHFRDFDHNLQLCVGNPRKTKSAFKGDSGGPLLCAGVAQGIVSYGRSDAKPPAVFTRISHYRPWINQILQAN

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Dog
83
Rabbit
70
Pig
0
Horse
0
Bovine
0
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P23946 作为底物

Site PTM Type Enzyme
S15 Phosphorylation
Y35 Phosphorylation
Y38 Phosphorylation
N80 N-Glycosylation
N103 N-Glycosylation

研究背景

功能:

Major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion.

细胞定位:

Secreted. Cytoplasmic granule.
Note: Mast cell granules.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Mast cells in lung, heart, skin and placenta. Expressed in both normal skin and in urticaria pigmentosa lesions.

蛋白家族:

Belongs to the peptidase S1 family. Granzyme subfamily.

研究领域

· Organismal Systems > Endocrine system > Renin-angiotensin system.   (View pathway)

文献引用

1). SYNPO2 promotes the development of BLCA by upregulating the infiltration of resting mast cells and increasing the resistance to immunotherapy. Oncology reports, 2024 (PubMed: 38038167) [IF=3.8]

Application: IF/ICC    Species: Mouse    Sample: lung tissue

Figure 5. SYNPO2 expression enhances mast cell infiltration. (A) SYNPO2 expression in 24 types of immune cell. Red text indicates a high correlation. (B) Representative toluidine and immunohistochemical staining in clinical Bladder Urothelial Carcinoma (BLCA) samples with low and high SYNPO2 expression. Purple represents cell degranulation. Scale bar, 100 µm. (C) Mast cell number/field in low and high SYNPO2 expression groups (P=0.0053). Correlation between SYNPO2 and (D) TPSAB1 and (E) CCR3 expression based on H-score. (F) Mouse serum IL-6 levels in NC (n=5) and SY (n=5; P=0.002). (G) Representative indirect immunofluorescence of a frozen lung section from a mouse model with pulmonary metastasis. The black arrow indicates the area of tumor invasion in the lung tissue. (H) Mouse serum TGF-β1 levels (P=0.045). (I) CMA-positive cells (n=3, P=0.0036). SYNPO2, synaptopodin-2; TPSAB1, Tryptase alpha/beta-1; CCR3, C-C chemokine receptor type 3; NC, Negative Control; SY, SYNPO2 overexpression; CMA1, Chymase 1. * and ** indicate P

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