产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF12291, RRID:AB_2845096.
展开/折叠
cytoplasmic; Cytosolic malate dehydrogenase; Diiodophenylpyruvate reductase; Malate dehydrogenase 1, NAD (soluble); Malate dehydrogenase; Malate dehydrogenase cytoplasmic; MDH s; mdh1; MDHA; MDHC_HUMAN; MDHs; MGC:1375; MOR2; Soluble malate dehydrogenase;
抗原和靶标
- P40925 MDHC_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSEPIRVLVTGAAGQIAYSLLYSIGNGSVFGKDQPIILVLLDITPMMGVLDGVLMELQDCALPLLKDVIATDKEDVAFKDLDVAILVGSMPRREGMERKDLLKANVKIFKSQGAALDKYAKKSVKVIVVGNPANTNCLTASKSAPSIPKENFSCLTRLDHNRAKAQIALKLGVTANDVKNVIIWGNHSSTQYPDVNHAKVKLQGKEVGVYEALKDDSWLKGEFVTTVQQRGAAVIKARKLSSAMSAAKAICDHVRDIWFGTPEGEFVSMGVISDGNSYGVPDDLLYSFPVVIKNKTWKFVEGLPINDFSREKMDLTAKELTEEKESAFEFLSSA
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P40925 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Acetylation | Uniprot | |
| Y22 | Phosphorylation | Uniprot | |
| T71 | Phosphorylation | Uniprot | |
| K73 | Acetylation | Uniprot | |
| K73 | Ubiquitination | Uniprot | |
| S89 | Phosphorylation | Uniprot | |
| K103 | Acetylation | Uniprot | |
| K103 | Ubiquitination | Uniprot | |
| K107 | Acetylation | Uniprot | |
| K107 | Ubiquitination | Uniprot | |
| K110 | Methylation | Uniprot | |
| K110 | Ubiquitination | Uniprot | |
| S111 | Phosphorylation | Uniprot | |
| K118 | Acetylation | Uniprot | |
| K118 | Ubiquitination | Uniprot | |
| Y119 | Phosphorylation | Uniprot | |
| K121 | Acetylation | Uniprot | |
| K125 | Ubiquitination | Uniprot | |
| C137 | S-Nitrosylation | Uniprot | |
| K142 | Acetylation | Uniprot | |
| K142 | Ubiquitination | Uniprot | |
| K149 | Ubiquitination | Uniprot | |
| C154 | S-Nitrosylation | Uniprot | |
| K164 | Ubiquitination | Uniprot | |
| K170 | Ubiquitination | Uniprot | |
| T174 | Phosphorylation | Uniprot | |
| K179 | Ubiquitination | Uniprot | |
| S188 | Phosphorylation | Uniprot | |
| S189 | Phosphorylation | Uniprot | |
| T190 | Phosphorylation | Uniprot | |
| Y192 | Phosphorylation | Uniprot | |
| K199 | Ubiquitination | Uniprot | |
| K205 | Ubiquitination | Uniprot | |
| Y210 | Phosphorylation | Uniprot | |
| K214 | Acetylation | Uniprot | |
| K214 | Ubiquitination | Uniprot | |
| S217 | Phosphorylation | Uniprot | |
| K220 | Ubiquitination | Uniprot | |
| R230 | Methylation | Uniprot | |
| K236 | Ubiquitination | Uniprot | |
| K239 | Ubiquitination | Uniprot | |
| S241 | Phosphorylation | Uniprot | |
| S242 | Phosphorylation | Uniprot | |
| S245 | Phosphorylation | Uniprot | |
| T296 | Phosphorylation | Uniprot | |
| K298 | Acetylation | Uniprot | |
| K298 | Ubiquitination | Uniprot | |
| S309 | Phosphorylation | Uniprot | |
| K312 | Ubiquitination | Uniprot | |
| K318 | Ubiquitination | Uniprot | |
| T321 | Phosphorylation | Uniprot | |
| K324 | Ubiquitination | Uniprot | |
| S326 | Phosphorylation | Uniprot | |
| S332 | Phosphorylation | Uniprot | |
| S333 | Phosphorylation | Uniprot |
研究背景
ISGylated.
Acetylation at Lys-118 dramatically enhances enzymatic activity and promotes adipogenic differentiation.
Cytoplasm.
Homodimer.
Belongs to the LDH/MDH superfamily. MDH type 2 family.
研究领域
· Metabolism > Carbohydrate metabolism > Citrate cycle (TCA cycle).
· Metabolism > Amino acid metabolism > Cysteine and methionine metabolism.
· Metabolism > Carbohydrate metabolism > Pyruvate metabolism.
· Metabolism > Carbohydrate metabolism > Glyoxylate and dicarboxylate metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
· Metabolism > Global and overview maps > Carbon metabolism.
· Organismal Systems > Excretory system > Proximal tubule bicarbonate reclamation.
限制条款
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