产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF12301, RRID:AB_2845106.
展开/折叠
CI 20; CI-20kD; Complex I 20kDa subunit; Complex I mitochondrial respiratory chain 20 KD subunit; Complex I-20kD; FLJ45860; FLJ46880; MGC120002; MY017; NADH coenzyme Q reductase; NADH dehydrogenase (ubiquinone) Fe S protein 7 20kDa (NADH coenzyme Q reductase); NADH dehydrogenase (ubiquinone) FeS protein 7, 20kDa (NADHcoenzyme Q reductase); NADH dehydrogenase (ubiquinone) FeS protein7, 20kDa (NADHcoenzyme Q reductase); NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial; NADH-ubiquinone oxidoreductase 20 kDa subunit; NADH:ubiquinone oxidoreductase PSST subunit; NADHcoenzyme Q reductase; Ndufs7; NDUS7_HUMAN; PSST; PSST subunit;
抗原和靶标
- O75251 NDUS7_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAVLSAPGLRGFRILGLRSSVGPAVQARGVHQSVATDGPSSTQPALPKARAVAPKPSSRGEYVVAKLDDLVNWARRSSLWPMTFGLACCAVEMMHMAAPRYDMDRFGVVFRASPRQSDVMIVAGTLTNKMAPALRKVYDQMPEPRYVVSMGSCANGGGYYHYSYSVVRGCDRIVPVDIYIPGCPPTAEALLYGILQLQRKIKRERRLQIWYRR
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O75251 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K48 | Ubiquitination | Uniprot | |
| K55 | Ubiquitination | Uniprot | |
| K66 | Ubiquitination | Uniprot | |
| R75 | Methylation | Uniprot | |
| S117 | Phosphorylation | Uniprot | |
| T125 | Phosphorylation | Uniprot | |
| T127 | Phosphorylation | Uniprot | |
| K129 | Ubiquitination | Uniprot | |
| K136 | Ubiquitination | Uniprot |
研究背景
Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Hydroxylated ar Arg-111 by NDUFAF5 early in the pathway of assembly of complex I, before the formation of the juncture between peripheral and membrane arms.
Mitochondrion.
Complex I is composed of 45 different subunits This is a component of the iron-sulfur (IP) fragment of the enzyme.
Belongs to the complex I 20 kDa subunit family.
研究领域
· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).
· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.
· Human Diseases > Neurodegenerative diseases > Parkinson's disease.
· Human Diseases > Neurodegenerative diseases > Huntington's disease.
· Metabolism > Energy metabolism > Oxidative phosphorylation.
· Metabolism > Global and overview maps > Metabolic pathways.
· Organismal Systems > Nervous system > Retrograde endocannabinoid signaling. (View pathway)
限制条款
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