| 产品: | RAP1B 抗体 |
| 货号: | DF12309 |
| 描述: | Rabbit polyclonal antibody to RAP1B |
| 应用: | WB IHC |
| 反应: | Human, Mouse, Rat |
| 预测: | Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 21 kDa; 21kD(Calculated). |
| 蛋白号: | P61224 |
| RRID: | AB_2845114 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF12309, RRID:AB_2845114.
展开/折叠
2810443E11Rik; DKFZp586H0723; GTP binding protein smg p21B; K-REV; MGC93206; RAL1B; RAP1B; RAP1B member of RAS oncogene family; Ras family small GTP binding protein RAP1B; RAS related protein 1b; Ras related protein Rap 1b; Ras related protein RAP1B;
抗原和靶标
- P61224 RAP1B_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MREYKLVVLGSGGVGKSALTVQFVQGIFVEKYDPTIEDSYRKQVEVDAQQCMLEILDTAGTEQFTAMRDLYMKNGQGFALVYSITAQSTFNDLQDLREQILRVKDTDDVPMILVGNKCDLEDERVVGKEQGQNLARQWNNCAFLESSAKSKINVNEIFYDLVRQINRKTPVPGKARKKSSCQLL
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P61224 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K5 | Ubiquitination | Uniprot | |
| S11 | Phosphorylation | Uniprot | |
| K31 | Ubiquitination | Uniprot | |
| Y32 | Phosphorylation | Uniprot | |
| S39 | Phosphorylation | Uniprot | |
| Y40 | Phosphorylation | Uniprot | |
| K42 | Ubiquitination | Uniprot | |
| K117 | Ubiquitination | Uniprot | |
| K128 | Ubiquitination | Uniprot | |
| K149 | Ubiquitination | Uniprot | |
| K151 | Ubiquitination | Uniprot | |
| Y159 | Phosphorylation | Uniprot | |
| K174 | Ubiquitination | Uniprot | |
| S179 | Phosphorylation | P17612 (PRKACA) , Q13976 (PRKG1) | Uniprot |
| S180 | Phosphorylation | Uniprot | |
| C181 | Methylation | Uniprot |
研究背景
GTP-binding protein that possesses intrinsic GTPase activity. Contributes to the polarizing activity of KRIT1 and CDH5 in the establishment and maintenance of correct endothelial cell polarity and vascular lumen. Required for the localization of phosphorylated PRKCZ, PARD3 and TIAM1 to the cell junction. Plays a role in the establishment of basal endothelial barrier function.
Cell membrane. Cytoplasm>Cytosol. Cell junction.
Note: May shuttle between plasma membrane and cytosol. Presence of KRIT1 and CDH5 is required for its localization to the cell junction.
Heterodimer with RAP1GAP. Interacts with EPAC2, SGSM1, SGSM2 and SGSM3. Interacts with KRIT1.
Belongs to the small GTPase superfamily. Ras family.
研究领域
· Cellular Processes > Cellular community - eukaryotes > Focal adhesion. (View pathway)
· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Ras signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Rap1 signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > cAMP signaling pathway. (View pathway)
· Human Diseases > Cancers: Specific types > Renal cell carcinoma. (View pathway)
· Organismal Systems > Immune system > Chemokine signaling pathway. (View pathway)
· Organismal Systems > Immune system > Platelet activation. (View pathway)
· Organismal Systems > Immune system > Leukocyte transendothelial migration. (View pathway)
· Organismal Systems > Nervous system > Long-term potentiation.
· Organismal Systems > Nervous system > Neurotrophin signaling pathway. (View pathway)
· Organismal Systems > Digestive system > Pancreatic secretion.
限制条款
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