产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF12329, RRID:AB_2845134.
展开/折叠
N-syndecan; Sdc3; SDC3_HUMAN; SDCN; SYND3; Syndecan neural type; syndecan proteoglycan 3; Syndecan-3;
抗原和靶标
- O75056 SDC3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MKPGPPHRAGAAHGAGAGAGAAAGPGARGLLLPPLLLLLLAGRAAGAQRWRSENFERPVDLEGSGDDDSFPDDELDDLYSGSGSGYFEQESGIETAMRFSPDVALAVSTTPAVLPTTNIQPVGTPFEELPSERPTLEPATSPLVVTEVPEEPSQRATTVSTTMATTAATSTGDPTVATVPATVATATPSTPAAPPFTATTAVIRTTGVRRLLPLPLTTVATARATTPEAPSPPTTAAVLDTEAPTPRLVSTATSRPRALPRPATTQEPDIPERSTLPLGTTAPGPTEVAQTPTPETFLTTIRDEPEVPVSGGPSGDFELPEEETTQPDTANEVVAVGGAAAKASSPPGTLPKGARPGPGLLDNAIDSGSSAAQLPQKSILERKEVLVAVIVGGVVGALFAAFLVTLLIYRMKKKDEGSYTLEEPKQASVTYQKPDKQEEFYA
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O75056 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T146 | Phosphorylation | Uniprot | |
| T234 | Phosphorylation | Uniprot | |
| T245 | Phosphorylation | Uniprot | |
| S250 | O-Glycosylation | Uniprot | |
| T251 | O-Glycosylation | Uniprot | |
| T253 | O-Glycosylation | Uniprot | |
| T264 | O-Glycosylation | Uniprot | |
| T265 | O-Glycosylation | Uniprot | |
| S344 | O-Glycosylation | Uniprot | |
| T349 | O-Glycosylation | Uniprot | |
| T349 | Phosphorylation | Uniprot | |
| Y409 | Phosphorylation | P54760 (EPHB4) | Uniprot |
| K414 | Ubiquitination | Uniprot | |
| S418 | Phosphorylation | Uniprot | |
| Y419 | Phosphorylation | P54760 (EPHB4) | Uniprot |
| S428 | Phosphorylation | Uniprot | |
| Y431 | Phosphorylation | P54760 (EPHB4) | Uniprot |
| K433 | Ubiquitination | Uniprot | |
| K436 | Ubiquitination | Uniprot | |
| Y441 | Phosphorylation | Uniprot |
研究背景
Cell surface proteoglycan that may bear heparan sulfate (By similarity). May have a role in the organization of cell shape by affecting the actin cytoskeleton, possibly by transferring signals from the cell surface in a sugar-dependent mechanism.
O-glycosylated within the Thr/Ser-rich region which could interact with lectin domains on other molecules.
Cell membrane>Single-pass type I membrane protein.
Expressed in the nervous system, the adrenal gland, and the spleen.
Interacts with TIAM1. Interacts with PTN (via heparan sulfate chains); this interaction mediates the neurite outgrowth-promoting signal from PTN to the cytoskeleton of growing neurites; this interaction mediates osteoblast recruitment (By similarity). Interacts with MDK; this interaction induces SDC3 clustering; this interaction induces neuronal cell adhesion and neurite outgrowth (By similarity).
Belongs to the syndecan proteoglycan family.
研究领域
· Environmental Information Processing > Signaling molecules and interaction > Cell adhesion molecules (CAMs). (View pathway)
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