(2)
(2)
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF12360, RRID:AB_2845165.
展开/折叠
Antigen nuclear dot 52 kDa protein; CACO2_HUMAN; Calcium binding and coiled coil domain 2; Calcium binding and coiled coil domain containing protein 2; Calcium-binding and coiled-coil domain-containing protein 2; CALCOCO 2; CALCOCO2; MGC17318; NDP52; Nuclear domain 10 protein 52; Nuclear domain 10 protein; Nuclear domain 10 protein NDP52; Nuclear domain protein 52; Nuclear dot protein 52; OTTHUMP00000218344; OTTHUMP00000218345; OTTHUMP00000218346; OTTHUMP00000218347; OTTHUMP00000218349; OTTHUMP00000218351;
抗原和靶标
A synthesized peptide derived from human CALCOCO2, corresponding to a region within the internal amino acids.
Expressed in all tissues tested with highest expression in skeletal muscle and lowest in brain.
- Q13137 CACO2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEETIKDPPTSAVLLDHCHFSQVIFNSVEKFYIPGGDVTCHYTFTQHFIPRRKDWIGIFRVGWKTTREYYTFMWVTLPIDLNNKSAKQQEVQFKAYYLPKDDEYYQFCYVDEDGVVRGASIPFQFRPENEEDILVVTTQGEVEEIEQHNKELCKENQELKDSCISLQKQNSDMQAELQKKQEELETLQSINKKLELKVKEQKDYWETELLQLKEQNQKMSSENEKMGIRVDQLQAQLSTQEKEMEKLVQGDQDKTEQLEQLKKENDHLFLSLTEQRKDQKKLEQTVEQMKQNETTAMKKQQELMDENFDLSKRLSENEIICNALQRQKERLEGENDLLKRENSRLLSYMGLDFNSLPYQVPTSDEGGARQNPGLAYGNPYSGIQESSSPSPLSIKKCPICKADDICDHTLEQQQMQPLCFNCPICDKIFPATEKQIFEDHVFCHSL
研究背景
Xenophagy-specific receptor required for autophagy-mediated intracellular bacteria degradation. Acts as an effector protein of galectin-sensed membrane damage that restricts the proliferation of infecting pathogens such as Salmonella typhimurium upon entry into the cytosol by targeting LGALS8-associated bacteria for autophagy. Initially orchestrates bacteria targeting to autophagosomes and subsequently ensures pathogen degradation by regulating pathogen-containing autophagosome maturation. Bacteria targeting to autophagosomes relies on its interaction with MAP1LC3A, MAP1LC3B and/or GABARAPL2, whereas regulation of pathogen-containing autophagosome maturation requires the interaction with MAP3LC3C. May play a role in ruffle formation and actin cytoskeleton organization and seems to negatively regulate constitutive secretion.
Cytoplasm>Perinuclear region. Cytoplasm>Cytoskeleton. Cytoplasmic vesicle>Autophagosome membrane>Peripheral membrane protein.
Note: According to PubMed:7540613, localizes to nuclear dots. According to PubMed:9230084 and PubMed:12869526, it is not a nuclear dot-associated protein but localizes predominantly in the cytoplasm with a coarse-grained distribution preferentially close to the nucleus.
Expressed in all tissues tested with highest expression in skeletal muscle and lowest in brain.
The MYO6-binding domain is required for autophagy-mediated degradation of infecting bacteria such as Salmonella typhimurium, but not for bacteria targeting to autophagosomes.
The CLIR (LC3C-interacting region) motif is required for interaction with MAP1LC3C, but dispensable for CALCOCO2-mediated autophagosome maturation.
The LIR-like motif is required for interaction with MAP1LC3A, MAP1LC3B and GABARAPL2, as well as for CALCOCO2-mediated autophagosome maturation.
The LGALS8-binding domain is essential for the recruitment to cytosol-exposed infecting bacteria.
Belongs to the CALCOCO family.
文献引用
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