(2)
(2)
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF12360, RRID:AB_2845165.
展开/折叠
Antigen nuclear dot 52 kDa protein; CACO2_HUMAN; Calcium binding and coiled coil domain 2; Calcium binding and coiled coil domain containing protein 2; Calcium-binding and coiled-coil domain-containing protein 2; CALCOCO 2; CALCOCO2; MGC17318; NDP52; Nuclear domain 10 protein 52; Nuclear domain 10 protein; Nuclear domain 10 protein NDP52; Nuclear domain protein 52; Nuclear dot protein 52; OTTHUMP00000218344; OTTHUMP00000218345; OTTHUMP00000218346; OTTHUMP00000218347; OTTHUMP00000218349; OTTHUMP00000218351;
抗原和靶标
Expressed in all tissues tested with highest expression in skeletal muscle and lowest in brain.
- Q13137 CACO2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEETIKDPPTSAVLLDHCHFSQVIFNSVEKFYIPGGDVTCHYTFTQHFIPRRKDWIGIFRVGWKTTREYYTFMWVTLPIDLNNKSAKQQEVQFKAYYLPKDDEYYQFCYVDEDGVVRGASIPFQFRPENEEDILVVTTQGEVEEIEQHNKELCKENQELKDSCISLQKQNSDMQAELQKKQEELETLQSINKKLELKVKEQKDYWETELLQLKEQNQKMSSENEKMGIRVDQLQAQLSTQEKEMEKLVQGDQDKTEQLEQLKKENDHLFLSLTEQRKDQKKLEQTVEQMKQNETTAMKKQQELMDENFDLSKRLSENEIICNALQRQKERLEGENDLLKRENSRLLSYMGLDFNSLPYQVPTSDEGGARQNPGLAYGNPYSGIQESSSPSPLSIKKCPICKADDICDHTLEQQQMQPLCFNCPICDKIFPATEKQIFEDHVFCHSL
翻译修饰 - Q13137 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K87 | Ubiquitination | Uniprot | |
| K94 | Ubiquitination | Uniprot | |
| Y96 | Phosphorylation | Uniprot | |
| K160 | Ubiquitination | Uniprot | |
| K168 | Ubiquitination | Uniprot | |
| K180 | Ubiquitination | Uniprot | |
| K192 | Ubiquitination | Uniprot | |
| Y204 | Phosphorylation | Uniprot | |
| K213 | Ubiquitination | Uniprot | |
| K225 | Ubiquitination | Uniprot | |
| S238 | Phosphorylation | Uniprot | |
| K242 | Ubiquitination | Uniprot | |
| K262 | Ubiquitination | Uniprot | |
| S271 | Phosphorylation | Uniprot | |
| K281 | Ubiquitination | Uniprot | |
| K299 | Ubiquitination | Uniprot | |
| K312 | Ubiquitination | Uniprot | |
| S315 | Phosphorylation | Uniprot | |
| K339 | Ubiquitination | Uniprot | |
| S347 | Phosphorylation | Uniprot | |
| S355 | Phosphorylation | Uniprot | |
| Y358 | Phosphorylation | Uniprot | |
| Y376 | Phosphorylation | Uniprot | |
| Y380 | Phosphorylation | Uniprot | |
| S381 | Phosphorylation | Uniprot | |
| S387 | Phosphorylation | Uniprot | |
| S388 | Phosphorylation | Uniprot | |
| S390 | Phosphorylation | Uniprot | |
| K395 | Ubiquitination | Uniprot | |
| K396 | Ubiquitination | Uniprot | |
| K401 | Ubiquitination | Uniprot | |
| S445 | Phosphorylation | Uniprot |
研究背景
Xenophagy-specific receptor required for autophagy-mediated intracellular bacteria degradation. Acts as an effector protein of galectin-sensed membrane damage that restricts the proliferation of infecting pathogens such as Salmonella typhimurium upon entry into the cytosol by targeting LGALS8-associated bacteria for autophagy. Initially orchestrates bacteria targeting to autophagosomes and subsequently ensures pathogen degradation by regulating pathogen-containing autophagosome maturation. Bacteria targeting to autophagosomes relies on its interaction with MAP1LC3A, MAP1LC3B and/or GABARAPL2, whereas regulation of pathogen-containing autophagosome maturation requires the interaction with MAP3LC3C. May play a role in ruffle formation and actin cytoskeleton organization and seems to negatively regulate constitutive secretion.
Cytoplasm>Perinuclear region. Cytoplasm>Cytoskeleton. Cytoplasmic vesicle>Autophagosome membrane>Peripheral membrane protein.
Note: According to PubMed:7540613, localizes to nuclear dots. According to PubMed:9230084 and PubMed:12869526, it is not a nuclear dot-associated protein but localizes predominantly in the cytoplasm with a coarse-grained distribution preferentially close to the nucleus.
Expressed in all tissues tested with highest expression in skeletal muscle and lowest in brain.
Dimer. Part of a complex consisting of CALCOCO2, TAX1BP1 and MYO6. Interacts with GEMIN4. Interacts with ATG8 family members MAP1LC3A, MAP1LC3B, GABARAP, GABARAPL1 and GABARAPL2. Interacts with ATG8 family member MAP1LC3C. Interacts with LGALS8.
The MYO6-binding domain is required for autophagy-mediated degradation of infecting bacteria such as Salmonella typhimurium, but not for bacteria targeting to autophagosomes.
The CLIR (LC3C-interacting region) motif is required for interaction with MAP1LC3C, but dispensable for CALCOCO2-mediated autophagosome maturation.
The LIR-like motif is required for interaction with MAP1LC3A, MAP1LC3B and GABARAPL2, as well as for CALCOCO2-mediated autophagosome maturation.
The LGALS8-binding domain is essential for the recruitment to cytosol-exposed infecting bacteria.
Belongs to the CALCOCO family.
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