产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF12366, RRID:AB_2845171.
展开/折叠
CCR4 NOT transcription complex subunit 10; CCR4-NOT transcription complex subunit 10; CNOT 10; CNOT10; CNOTA_HUMAN; DKFZp434K115; FLJ12890; FLJ13165;
抗原和靶标
- Q9H9A5 CNO10_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAADKPADQGAEKHEGTGQSSGITDQEKELSTNAFQAFTSGNYDACLQHLACLQDINKDDYKIILNTAVAEFFKSNQTTTDNLRQTLNQLKNQVHSAVEEMDGLDDVENSMLYYNQAVILYHLRQYTEAISVGEKLYQFIEPFEEKFAQAVCFLLVDLYILTYQAEKALHLLAVLEKMISQGNNNKNGKNETGNNNNKDGSNHKAESGALIEAAKSKIHQYKVRAYIQMKSLKACKREIKSVMNTAGNSAPSLFLKSNFEYLRGNYRKAVKLLNSSNIAEHPGFMKTGECLRCMFWNNLGCIHFAMSKHNLGIFYFKKALQENDNVCAQLSAGSTDPGKKFSGRPMCTLLTNKRYELLYNCGIQLLHIGRPLAAFECLIEAVQVYHANPRLWLRLAECCIAANKGTSEQETKGLPSKKGIVQSIVGQGYHRKIVLASQSIQNTVYNDGQSSAIPVASMEFAAICLRNALLLLPEEQQDPKQENGAKNSNQLGGNTESSESSETCSSKSHDGDKFIPAPPSSPLRKQELENLKCSILACSAYVALALGDNLMALNHADKLLQQPKLSGSLKFLGHLYAAEALISLDRISDAITHLNPENVTDVSLGISSNEQDQGSDKGENEAMESSGKRAPQCYPSSVNSARTVMLFNLGSAYCLRSEYDKARKCLHQAASMIHPKEVPPEAILLAVYLELQNGNTQLALQIIKRNQLLPAVKTHSEVRKKPVFQPVHPIQPIQMPAFTTVQRK
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9H9A5 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| K28 | Ubiquitination | Uniprot | |
| K58 | Ubiquitination | Uniprot | |
| K74 | Ubiquitination | Uniprot | |
| T162 | Phosphorylation | Uniprot | |
| K177 | Acetylation | Uniprot | |
| K204 | Ubiquitination | Uniprot | |
| K215 | Ubiquitination | Uniprot | |
| K217 | Ubiquitination | Uniprot | |
| K230 | Ubiquitination | Uniprot | |
| K240 | Ubiquitination | Uniprot | |
| S241 | Phosphorylation | Uniprot | |
| T245 | Phosphorylation | Uniprot | |
| S249 | Phosphorylation | Uniprot | |
| K256 | Acetylation | Uniprot | |
| K256 | Methylation | Uniprot | |
| K256 | Ubiquitination | Uniprot | |
| R263 | Methylation | Uniprot | |
| K271 | Ubiquitination | Uniprot | |
| K286 | Ubiquitination | Uniprot | |
| K318 | Ubiquitination | Uniprot | |
| K339 | Ubiquitination | Uniprot | |
| K404 | Ubiquitination | Uniprot | |
| K412 | Ubiquitination | Uniprot | |
| K418 | Ubiquitination | Uniprot | |
| K480 | Ubiquitination | Uniprot | |
| C504 | S-Nitrosylation | Uniprot | |
| K513 | Ubiquitination | Uniprot | |
| S521 | Phosphorylation | Uniprot | |
| K525 | Ubiquitination | Uniprot | |
| K564 | Ubiquitination | Uniprot | |
| C633 | S-Nitrosylation | Uniprot | |
| K664 | Ubiquitination | Uniprot | |
| K713 | Ubiquitination | Uniprot |
研究背景
Component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. Is not required for association of CNOT7 to the CCR4-NOT complex.
Cytoplasm. Nucleus.
Component of the CCR4-NOT complex; distinct complexes seem to exist that differ in the participation of probably mutually exclusive catalytic subunits. CNOT10 and CNOT11 form a subcomplex docked to the CNOT1 scaffold.
Belongs to the CNOT10 family.
研究领域
· Genetic Information Processing > Folding, sorting and degradation > RNA degradation.
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