(8)
(7)
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF6233, RRID:AB_2834800.
展开/折叠
GATA 3; GATA binding factor 3; GATA binding protein 3; GATA-binding factor 3; Gata3; GATA3_HUMAN; HDR; HDRS; MGC2346; MGC5199; MGC5445; Trans acting T cell specific transcription factor GATA 3; Trans-acting T-cell-specific transcription factor GATA-3; DCML; Endothelial transcription factor GATA 2; Endothelial transcription factor GATA-2; Endothelial transcription factor GATA2; FLJ45948; GATA 2; GATA binding protein 2; GATA-binding protein 2; Gata2; GATA2_HUMAN; IMD21; MGC2306; MONOMAC; NFE 1B; NFE1B; OTTHUMP00000216240;
抗原和靶标
- P23771 GATA3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEVTADQPRWVSHHHPAVLNGQHPDTHHPGLSHSYMDAAQYPLPEEVDVLFNIDGQGNHVPPYYGNSVRATVQRYPPTHHGSQVCRPPLLHGSLPWLDGGKALGSHHTASPWNLSPFSKTSIHHGSPGPLSVYPPASSSSLSGGHASPHLFTFPPTPPKDVSPDPSLSTPGSAGSARQDEKECLKYQVPLPDSMKLESSHSRGSMTALGGASSSTHHPITTYPPYVPEYSSGLFPPSSLLGGSPTGFGCKSRPKARSSTGRECVNCGATSTPLWRRDGTGHYLCNACGLYHKMNGQNRPLIKPKRRLSAARRAGTSCANCQTTTTTLWRRNANGDPVCNACGLYYKLHNINRPLTMKKEGIQTRNRKMSSKSKKCKKVHDSLEDFPKNSSFNPAALSRHMSSLSHISPFSHSSHMLTTPTPMHPPSSLSFGPHHPSSMVTAMG
- P23769 GATA2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEVAPEQPRWMAHPAVLNAQHPDSHHPGLAHNYMEPAQLLPPDEVDVFFNHLDSQGNPYYANPAHARARVSYSPAHARLTGGQMCRPHLLHSPGLPWLDGGKAALSAAAAHHHNPWTVSPFSKTPLHPSAAGGPGGPLSVYPGAGGGSGGGSGSSVASLTPTAAHSGSHLFGFPPTPPKEVSPDPSTTGAASPASSSAGGSAARGEDKDGVKYQVSLTESMKMESGSPLRPGLATMGTQPATHHPIPTYPSYVPAAAHDYSSGLFHPGGFLGGPASSFTPKQRSKARSCSEGRECVNCGATATPLWRRDGTGHYLCNACGLYHKMNGQNRPLIKPKRRLSAARRAGTCCANCQTTTTTLWRRNANGDPVCNACGLYYKLHNVNRPLTMKKEGIQTRNRKMSNKSKKSKKGAECFEELSKCMQEKSSPFSAAALAGHMAPVGHLPPFSHSGHILPTPTPIHPSSSLSFGHPHPSSMVTAMG
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P23771/P23769 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Ubiquitination | Uniprot | ||
| S110 | Phosphorylation | Uniprot | |
| S115 | Phosphorylation | Uniprot | |
| T156 | Phosphorylation | P24941 (CDK2) | Uniprot |
| S162 | Phosphorylation | Uniprot | |
| S166 | Phosphorylation | Uniprot | |
| S172 | Phosphorylation | Uniprot | |
| Y186 | Phosphorylation | Uniprot | |
| K195 | Ubiquitination | Uniprot | |
| R261 | Methylation | Uniprot | |
| T271 | Phosphorylation | Uniprot | |
| Y282 | Phosphorylation | Uniprot | |
| Y290 | Phosphorylation | Uniprot | |
| K292 | Acetylation | Uniprot | |
| K304 | Acetylation | Uniprot | |
| S308 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| T315 | Phosphorylation | Uniprot | |
| S316 | Phosphorylation | Uniprot | |
| Y344 | Phosphorylation | Uniprot | |
| K346 | Acetylation | Uniprot | |
| S369 | Phosphorylation | Uniprot | |
| K371 | Acetylation | Uniprot | |
| K373 | Acetylation | Uniprot | |
| K374 | Acetylation | Uniprot | |
| K376 | Acetylation | Uniprot | |
| K377 | Ubiquitination | Uniprot | |
| S381 | Phosphorylation | Uniprot |
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S71 | Phosphorylation | Uniprot | |
| S73 | Phosphorylation | Uniprot | |
| R86 | Methylation | Uniprot | |
| S119 | Phosphorylation | Uniprot | |
| T176 | Phosphorylation | P06493 (CDK1) | Uniprot |
| S182 | Phosphorylation | Uniprot | |
| S186 | Phosphorylation | Uniprot | |
| S192 | Phosphorylation | Uniprot | |
| Y213 | Phosphorylation | Uniprot | |
| S216 | Phosphorylation | Uniprot | |
| S220 | Phosphorylation | Uniprot | |
| S276 | Phosphorylation | Uniprot | |
| S290 | Phosphorylation | Uniprot | |
| Y314 | Phosphorylation | Uniprot | |
| Y322 | Phosphorylation | Uniprot | |
| Y376 | Phosphorylation | Uniprot | |
| K389 | Sumoylation | Uniprot | |
| S401 | Phosphorylation | P31749 (AKT1) | Uniprot |
| K409 | Ubiquitination | Uniprot |
研究背景
Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'. Required for the T-helper 2 (Th2) differentiation process following immune and inflammatory responses.
Nucleus.
T-cells and endothelial cells.
Interacts with TBX21 ('Tyr-530' phosphorylated form).
Binds DNA via the 2 GATA-type zinc fingers. Each zinc finger may bind either adjacent sites in a palindromic motif, or a different DNA molecule allowing looping and long-range gene regulation.
The YxKxHxxxRP motif is critical for DNA-binding and function.
Transcriptional activator which regulates endothelin-1 gene expression in endothelial cells. Binds to the consensus sequence 5'-AGATAG-3'.
Nucleus.
Endothelial cells.
Interacts with BRD3 (By similarity). Interacts with AR and CCAR1.
研究领域
· Human Diseases > Immune diseases > Inflammatory bowel disease (IBD).
· Organismal Systems > Immune system > Th1 and Th2 cell differentiation. (View pathway)
· Organismal Systems > Immune system > Th17 cell differentiation. (View pathway)
文献引用
Application: WB Species: Mouse Sample:
Application: WB Species: Mouse Sample:
Application: WB Species: human Sample: HT-29 and DLD1 cells
Application: IHC Species: Human Sample: SCC tissues
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