| 产品: | DAAM1 抗体 |
| 货号: | DF12380 |
| 描述: | Rabbit polyclonal antibody to DAAM1 |
| 应用: | WB IHC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken |
| 分子量: | 123 kDa; 123kD(Calculated). |
| 蛋白号: | Q9Y4D1 |
| RRID: | AB_2845185 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF12380, RRID:AB_2845185.
展开/折叠
DAAM 1; Dishevelled associated activator of morphogenesis 1; FLJ41657; KIAA0666;
抗原和靶标
- Q9Y4D1 DAAM1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAPRKRGGRGISFIFCCFRNNDHPEITYRLRNDSNFALQTMEPALPMPPVEELDVMFSELVDELDLTDKHREAMFALPAEKKWQIYCSKKKDQEENKGATSWPEFYIDQLNSMAARKSLLALEKEEEEERSKTIESLKTALRTKPMRFVTRFIDLDGLSCILNFLKTMDYETSESRIHTSLIGCIKALMNNSQGRAHVLAHSESINVIAQSLSTENIKTKVAVLEILGAVCLVPGGHKKVLQAMLHYQKYASERTRFQTLINDLDKSTGRYRDEVSLKTAIMSFINAVLSQGAGVESLDFRLHLRYEFLMLGIQPVIDKLREHENSTLDRHLDFFEMLRNEDELEFAKRFELVHIDTKSATQMFELTRKRLTHSEAYPHFMSILHHCLQMPYKRSGNTVQYWLLLDRIIQQIVIQNDKGQDPDSTPLENFNIKNVVRMLVNENEVKQWKEQAEKMRKEHNELQQKLEKKERECDAKTQEKEEMMQTLNKMKEKLEKETTEHKQVKQQVADLTAQLHELSRRAVCASIPGGPSPGAPGGPFPSSVPGSLLPPPPPPPLPGGMLPPPPPPLPPGGPPPPPGPPPLGAIMPPPGAPMGLALKKKSIPQPTNALKSFNWSKLPENKLEGTVWTEIDDTKVFKILDLEDLERTFSAYQRQQDFFVNSNSKQKEADAIDDTLSSKLKVKELSVIDGRRAQNCNILLSRLKLSNDEIKRAILTMDEQEDLPKDMLEQLLKFVPEKSDIDLLEEHKHELDRMAKADRFLFEMSRINHYQQRLQSLYFKKKFAERVAEVKPKVEAIRSGSEEVFRSGALKQLLEVVLAFGNYMNKGQRGNAYGFKISSLNKIADTKSSIDKNITLLHYLITIVENKYPSVLNLNEELRDIPQAAKVNMTELDKEISTLRSGLKAVETELEYQKSQPPQPGDKFVSVVSQFITVASFSFSDVEDLLAEAKDLFTKAVKHFGEEAGKIQPDEFFGIFDQFLQAVSEAKQENENMRKKKEEEERRARMEAQLKEQRERERKMRKAKENSEESGEFDDLVSALRSGEVFDKDLSKLKRNRKRITNQMTDSSRERPITKLNF
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9Y4D1 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| R9 | Methylation | Uniprot | |
| S12 | Phosphorylation | Uniprot | |
| T27 | Phosphorylation | Uniprot | |
| Y28 | Phosphorylation | Uniprot | |
| S34 | Phosphorylation | Uniprot | |
| K97 | Ubiquitination | Uniprot | |
| S118 | Phosphorylation | Uniprot | |
| K124 | Ubiquitination | Uniprot | |
| K132 | Ubiquitination | Uniprot | |
| K186 | Acetylation | Uniprot | |
| Y247 | Phosphorylation | Uniprot | |
| T255 | Phosphorylation | Uniprot | |
| T259 | Phosphorylation | Uniprot | |
| Y271 | Phosphorylation | Uniprot | |
| Y401 | Phosphorylation | Uniprot | |
| T425 | Phosphorylation | Uniprot | |
| K433 | Ubiquitination | Uniprot | |
| K476 | Acetylation | Uniprot | |
| K489 | Acetylation | Uniprot | |
| K491 | Acetylation | Uniprot | |
| T499 | Phosphorylation | Uniprot | |
| K638 | Ubiquitination | Uniprot | |
| Y652 | Phosphorylation | Uniprot | |
| K679 | Ubiquitination | Uniprot | |
| K748 | Ubiquitination | Uniprot | |
| S776 | Phosphorylation | Uniprot | |
| S799 | Phosphorylation | Uniprot | |
| S801 | Phosphorylation | Uniprot | |
| Y833 | Phosphorylation | Uniprot | |
| K842 | Ubiquitination | Uniprot | |
| Y912 | Phosphorylation | Uniprot | |
| S1027 | Phosphorylation | Uniprot | |
| T1061 | Phosphorylation | Uniprot | |
| T1074 | Phosphorylation | Uniprot |
研究背景
Binds to disheveled (Dvl) and Rho, and mediates Wnt-induced Dvl-Rho complex formation. May play a role as a scaffolding protein to recruit Rho-GDP and Rho-GEF, thereby enhancing Rho-GTP formation. Can direct nucleation and elongation of new actin filaments. Involved in building functional cilia. Involved in the organization of the subapical actin network in multiciliated epithelial cells (By similarity). Together with DAAM2, required for myocardial maturation and sarcomere assembly (By similarity).
Cytoplasm. Cytoplasm>Cytoskeleton>Cilium basal body.
Note: Perinuclear.
Expressed in all tissues examined.
Homodimer. Interacts with CIP4, FNBP1 and FNBP1L. Interacts with the SH3 domains of Abl, BTK, endophilin, spectrin and SRC. Binds specifically to GTP-bound CDC42 and RHOA. Interacts with INTU; INTU mediates the indirect interaction between DAAM1 and NPHP4.
The C-terminal DAD domain may participate in intramolecular interactions with the N-terminus.
The DAD domain regulates activation via by an autoinhibitory interaction with the GBD/FH3 domain. This autoinhibition is released upon competitive binding of an activated GTPase. The release of DAD allows the FH2 domain to then nucleate and elongate nonbranched actin filaments (By similarity).
Belongs to the formin homology family.
研究领域
· Environmental Information Processing > Signal transduction > Wnt signaling pathway. (View pathway)
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