E2F3 Antibody - #DF12390

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产品: E2F3 抗体
货号: DF12390
描述: Rabbit polyclonal antibody to E2F3
应用: WB IHC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
分子量: 60-65 kDa; 49kD(Calculated).
蛋白号: O00716
RRID: AB_2845195

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(92%)
克隆:
Polyclonal
特异性:
E2F3 Antibody detects endogenous levels of total E2F3.
RRID:
AB_2845195
引用格式: Affinity Biosciences Cat# DF12390, RRID:AB_2845195.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

DKFZp686C18211; E2F 3; E2F transcription factor 3; E2F-3; E2F3; E2F3_HUMAN; KIAA0075; MGC104598; Transcription factor E2F 3; Transcription factor E2F3;

抗原和靶标

免疫原:
Uniprot:

O00716(E2F3_HUMAN) >>Visit HPA database.

基因/基因ID:
E2F3(1871)
序列:
MRKGIQPALEQYLVTAGGGEGAAVVAAAAAASMDKRALLASPGFAAAAAAAAAPGAYIQILTTNTSTTSCSSSLQSGAVAAGPLLPSAPGAEQTAGSLLYTTPHGPSSRAGLLQQPPALGRGGSGGGGGPPAKRRLELGESGHQYLSDGLKTPKGKGRAALRSPDSPKTPKSPSEKTRYDTSLGLLTKKFIQLLSQSPDGVLDLNKAAEVLKVQKRRIYDITNVLEGIHLIKKKSKNNVQWMGCSLSEDGGMLAQCQGLSKEVTELSQEEKKLDELIQSCTLDLKLLTEDSENQRLAYVTYQDIRKISGLKDQTVIVVKAPPETRLEVPDSIESLQIHLASTQGPIEVYLCPEETETHSPMKTNNQDHNGNIPKPASKDLASTNSGHSDCSVSMGNLSPLASPANLLQQTEDQIPSNLEGPFVNLLPPLLQEDYLLSLGEEEGISDLFDAYDLEKLPLVEDFMCS

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
92
Zebrafish
73
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - O00716 作为底物

Site PTM Type Enzyme
Phosphorylation
R121 Methylation
S124 Phosphorylation O14757 (CHEK1) , O96017 (CHEK2)
T152 Phosphorylation
S163 Phosphorylation
S166 Phosphorylation
K168 Acetylation
T169 Phosphorylation
K171 Acetylation
S172 Phosphorylation
S174 Phosphorylation
K176 Acetylation
Y179 Phosphorylation
S182 Phosphorylation
K188 Ubiquitination
K206 Ubiquitination
K212 Ubiquitination
S235 Phosphorylation
S267 Phosphorylation
K306 Acetylation
Y349 Phosphorylation

研究背景

功能:

Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F3 binds specifically to RB1 in a cell-cycle dependent manner. Inhibits adipogenesis, probably through the repression of CEBPA binding to its target gene promoters (By similarity).

细胞定位:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Component of the DRTF1/E2F transcription factor complex. Binds cooperatively with TFDP1/Dp-1 to E2F sites. Interacts with retinoblastoma protein RB1 and related proteins (such as RBL1) that inhibit the E2F transactivation domain. Binds EAPP.

蛋白家族:

Belongs to the E2F/DP family.

研究领域

· Cellular Processes > Cell growth and death > Cell cycle.   (View pathway)

· Cellular Processes > Cell growth and death > Cellular senescence.   (View pathway)

· Human Diseases > Drug resistance: Antineoplastic > Endocrine resistance.

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

· Human Diseases > Cancers: Specific types > Pancreatic cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Glioma.   (View pathway)

· Human Diseases > Cancers: Specific types > Prostate cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Melanoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Bladder cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Chronic myeloid leukemia.   (View pathway)

· Human Diseases > Cancers: Specific types > Small cell lung cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Non-small cell lung cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Breast cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Gastric cancer.   (View pathway)

文献引用

1). IGF2BP3 enhances the mRNA stability of E2F3 by interacting with LINC00958 to promote endometrial carcinoma progression. Cell Death Discovery, 2022 (PubMed: 35676262) [IF=7.0]
2). CircRHOT1 Mediated Cell Proliferation, Apoptosis and Invasion of Pancreatic Cancer Cells by Sponging miR125a-3p. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE, 2020 (PubMed: 32697386) [IF=5.3]

Application: WB    Species: human    Sample: HPDE and PANC-1 cells

FIGURE 4|CircRHOT1 regulates the expression of E2F3 by targeting miR-125a-3p in PANC-1 cells.F and G, Relative expression of E2F3mRNA and protein in human pancreatic ductal cell line (HPDE) and PANC-1 cells was measured by RT-qPCR.
3). Long non-coding RNA NEAT1 regulates E2F3 expression by competitively binding to miR-377 in non-small cell lung cancer. Oncology Letters, 2017 (PubMed: 29085511) [IF=2.9]

Application: WB    Species: human    Sample: A549 cells

Figure 3. |NEAT1 promotes E2F3 expression in part through competitive binding miRNA‑377‑3p. The relative level of miR‑377‑3p in A549 and H1299 (A). The luciferase report assay demonstrated that miR‑377‑3p could reduce the intensity of fluorescence in A549 (B) and H1299 (C) transfected with the NEAT1‑WT vector. Luciferase reporter assays indicated that luciferase activity generated by the reporter vector with E2F3‑3'‑WT decreased more than 50% after co‑transfected with miR‑377‑3p in both A549 (D) and H1299 (E). Overexpression of miR‑377‑3p inhibited NEAT1 expression (F). Overexpression of miR‑377‑3p inhibited E2F expression in A549 (G).

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