EXOC5 Antibody - #DF12397

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产品: EXOC5 抗体
货号: DF12397
描述: Rabbit polyclonal antibody to EXOC5
应用: WB IHC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 77 kDa; 82kD(Calculated).
蛋白号: O00471
RRID: AB_2845202

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(92%), Bovine(92%), Horse(100%), Sheep(92%), Rabbit(100%), Dog(100%), Chicken(92%)
克隆:
Polyclonal
特异性:
EXOC5 Antibody detects endogenous levels of total EXOC5.
RRID:
AB_2845202
引用格式: Affinity Biosciences Cat# DF12397, RRID:AB_2845202.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

AI447711; AI448003; DKFZp666H126; EXOC5; exocyst complex component 5; Exocyst complex component Sec10; Gm76; HSEC10; p71 component of rsec6/8 secretory complex; PRO1912; SEC10; SEC10 like 1; SEC10L1; SEC10P;

抗原和靶标

免疫原:
Uniprot:

O00471(EXOC5_HUMAN) >>Visit HPA database.

基因/基因ID:
EXOC5(10640)
表达:
O00471 EXOC5_HUMAN:

Ubiquitous.

序列:
MATTAELFEEPFVADEYIERLVWRTPGGGSRGGPEAFDPKRLLEEFVNHIQELQIMDERIQRKVEKLEQQCQKEAKEFAKKVQELQKSNQVAFQHFQELDEHISYVATKVCHLGDQLEGVNTPRQRAVEAQKLMKYFNEFLDGELKSDVFTNSEKIKEAADIIQKLHLIAQELPFDRFSEVKSKIASKYHDLECQLIQEFTSAQRRGEISRMREVAAVLLHFKGYSHCVDVYIKQCQEGAYLRNDIFEDAGILCQRVNKQVGDIFSNPETVLAKLIQNVFEIKLQSFVKEQLEECRKSDAEQYLKNLYDLYTRTTNLSSKLMEFNLGTDKQTFLSKLIKSIFISYLENYIEVETGYLKSRSAMILQRYYDSKNHQKRSIGTGGIQDLKERIRQRTNLPLGPSIDTHGETFLSQEVVVNLLQETKQAFERCHRLSDPSDLPRNAFRIFTILVEFLCIEHIDYALETGLAGIPSSDSRNANLYFLDVVQQANTIFHLFDKQFNDHLMPLISSSPKLSECLQKKKEIIEQMEMKLDTGIDRTLNCMIGQMKHILAAEQKKTDFKPEDENNVLIQYTNACVKVCAYVRKQVEKIKNSMDGKNVDTVLMELGVRFHRLIYEHLQQYSYSCMGGMLAICDVAEYRKCAKDFKIPMVLHLFDTLHALCNLLVVAPDNLKQVCSGEQLANLDKNILHSFVQLRADYRSARLARHFS

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Horse
100
Dog
100
Rabbit
100
Pig
92
Bovine
92
Sheep
92
Chicken
92
Xenopus
73
Zebrafish
58
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - O00471 作为底物

Site PTM Type Enzyme
A2 Acetylation
T3 Phosphorylation
T4 Phosphorylation
K87 Ubiquitination
T122 Phosphorylation
K132 Ubiquitination
K135 Ubiquitination
K146 Ubiquitination
S147 Phosphorylation
K155 Ubiquitination
K157 Ubiquitination
K165 Ubiquitination
R177 Methylation
S210 Phosphorylation
Y225 Phosphorylation
K259 Ubiquitination
K289 Ubiquitination
K297 Ubiquitination
T328 Phosphorylation
T332 Phosphorylation
S378 Phosphorylation
K388 Ubiquitination
K557 Ubiquitination

研究背景

功能:

Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane.

细胞定位:

Cytoplasm. Midbody.
Note: Localization at the midbody requires the presence of RALA, EXOC2 and EXOC3.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Ubiquitous.

亚基结构:

The exocyst complex is composed of EXOC1, EXOC2, EXOC3, EXOC4, EXOC5, EXOC6, EXOC7 and EXOC8 (By similarity). Interacts with EXOC3L1 (By similarity).

蛋白家族:

Belongs to the SEC10 family.

文献引用

1). Exocyst controls exosome biogenesis via Rab11a. Molecular Therapy-Nucleic Acids, 2022 (PubMed: 35036064) [IF=6.5]

Application: IHC    Species: Human    Sample: LC tissue

Figure 1 Regulation of exosome release by exocysts in HNC (A and B) Sec10, Sec3, and Exo70 expression in LC tissue compared with that in Ctrl. (A) Representative images of immunohistochemistry staining. (B) Data summary of immunohistochemistry staining. LC, laryngocarcinoma tissue; Ctrl, adjacent normal tissues. ∗∗p < 0.01, ∗∗∗p < 0.001 by t test. Scale bar, 50 μm. (C and D) Sec10, Sec3, and Exo70 expression in HN4 and NP69 cells. (C) Representative images of western blotting. (D) The summary data of western blotting compared with NP69 cells. (E and F) Representative NTA traces and quantification of exosomes after knockdown of the expression of exocyst subunits by transfecting HN4 (E) and CNE2 (F) cells with specific small interfering (si)RNA for Sec10, Sec3, or Exo70 protein. ∗p < 0.05, ∗∗p < 0.05, ∗∗∗p < 0.001 by t test.

Application: WB    Species: Human    Sample: HN4 and NP69 cells

Figure 1 Regulation of exosome release by exocysts in HNC (A and B) Sec10, Sec3, and Exo70 expression in LC tissue compared with that in Ctrl. (A) Representative images of immunohistochemistry staining. (B) Data summary of immunohistochemistry staining. LC, laryngocarcinoma tissue; Ctrl, adjacent normal tissues. ∗∗p < 0.01, ∗∗∗p < 0.001 by t test. Scale bar, 50 μm. (C and D) Sec10, Sec3, and Exo70 expression in HN4 and NP69 cells. (C) Representative images of western blotting. (D) The summary data of western blotting compared with NP69 cells. (E and F) Representative NTA traces and quantification of exosomes after knockdown of the expression of exocyst subunits by transfecting HN4 (E) and CNE2 (F) cells with specific small interfering (si)RNA for Sec10, Sec3, or Exo70 protein. ∗p < 0.05, ∗∗p < 0.05, ∗∗∗p < 0.001 by t test.

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