产品: GSTM4 抗体
货号: DF12409
描述: Rabbit polyclonal antibody to GSTM4
应用: WB IHC IF/ICC
反应: Human, Mouse
预测: Rabbit
分子量: 26 kDa; 26kD(Calculated).
蛋白号: Q03013
RRID: AB_2845214

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse
预测:
Rabbit(100%)
克隆:
Polyclonal
特异性:
GSTM4 Antibody detects endogenous levels of total GSTM4.
RRID:
AB_2845214
引用格式: Affinity Biosciences Cat# DF12409, RRID:AB_2845214.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Glutathione S transferase M4; Glutathione S transferase Mu 4; Glutathione S-transferase Mu 4; GST class mu 4; GST class-mu 4; GST-Mu2; GSTM4 4; GSTM4; GSTM4-4; GSTM4_HUMAN; GTM4; GTS Mu2; MGC131945; MGC9247;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
Q03013 GSTM4_HUMAN:

Expressed in a wide variety of tissues.

序列:
MSMTLGYWDIRGLAHAIRLLLEYTDSSYEEKKYTMGDAPDYDRSQWLNEKFKLGLDFPNLPYLIDGAHKITQSNAILCYIARKHNLCGETEEEKIRVDILENQAMDVSNQLARVCYSPDFEKLKPEYLEELPTMMQHFSQFLGKRPWFVGDKITFVDFLAYDVLDLHRIFEPNCLDAFPNLKDFISRFEGLEKISAYMKSSRFLPKPLYTRVAVWGNK

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Rabbit
100
Horse
73
Bovine
60
Pig
0
Sheep
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q03013 作为底物

Site PTM Type Enzyme
Y23 Phosphorylation
S26 Phosphorylation
K31 Ubiquitination
K32 Ubiquitination
R43 Methylation
K50 Ubiquitination
K52 Ubiquitination
Y62 Phosphorylation
T71 Phosphorylation
S73 Phosphorylation
Y79 Phosphorylation
K83 Ubiquitination
K94 Ubiquitination
K182 Ubiquitination
S186 Phosphorylation
K193 Ubiquitination
K199 Acetylation
K199 Ubiquitination
K206 Ubiquitination

研究背景

功能:

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Active on 1-chloro-2,4-dinitrobenzene.

细胞定位:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed in a wide variety of tissues.

亚基结构:

Homodimer.

蛋白家族:

Belongs to the GST superfamily. Mu family.

研究领域

· Human Diseases > Drug resistance: Antineoplastic > Platinum drug resistance.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Chemical carcinogenesis.

· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma.   (View pathway)

· Metabolism > Metabolism of other amino acids > Glutathione metabolism.

· Metabolism > Xenobiotics biodegradation and metabolism > Metabolism of xenobiotics by cytochrome P450.

· Metabolism > Xenobiotics biodegradation and metabolism > Drug metabolism - cytochrome P450.

· Metabolism > Xenobiotics biodegradation and metabolism > Drug metabolism - other enzymes.

文献引用

1). A quantitative proteomic study reveals oxidative stress and synapse-related proteins contributed to TDCIPP exposure induced neurotoxicity. Ecotoxicology and environmental safety, 2024 (PubMed: 38262093) [IF=6.8]

Application: WB    Species: Rat    Sample:

Fig. 5. Dysregulated proteins induced by TDCIPP exposure were analyzed by heatmap and Western blot. (A) The heatmap is clustered in rows and the values are protein expression abundance, with blue representing down-regulated proteins and red representing up-regulated proteins. Western blot analysis and quantification of PKN1 (B and F), GSTM4 (C and G), BMAL1 (D and H), NQO1 (E and I) expression levels. Data was shown Mean ± SEM. * , p 

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