产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF12415, RRID:AB_2845220.
展开/折叠
Ankyrin repeat-rich membrane-spanning protein; arms; KDIS_HUMAN; kidins220; Kinase D interacting substance of 220 kDa; Kinase D-interacting substrate of 220 kDa; rgd 619949;
抗原和靶标
Abundant in developing and adult neural tissues as well as neuroendocrine cells and dendritic cells. Overexpressed in melanoma and melanoma cell lines.
- Q9ULH0 KDIS_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSVLISQSVINYVEEENIPALKALLEKCKDVDERNECGQTPLMIAAEQGNLEIVKELIKNGANCNLEDLDNWTALISASKEGHVHIVEELLKCGVNLEHRDMGGWTALMWACYKGRTDVVELLLSHGANPSVTGLYSVYPIIWAAGRGHADIVHLLLQNGAKVNCSDKYGTTPLVWAARKGHLECVKHLLAMGADVDQEGANSMTALIVAVKGGYTQSVKEILKRNPNVNLTDKDGNTALMIASKEGHTEIVQDLLDAGTYVNIPDRSGDTVLIGAVRGGHVEIVRALLQKYADIDIRGQDNKTALYWAVEKGNATMVRDILQCNPDTEICTKDGETPLIKATKMRNIEVVELLLDKGAKVSAVDKKGDTPLHIAIRGRSRKLAELLLRNPKDGRLLYRPNKAGETPYNIDCSHQKSILTQIFGARHLSPTETDGDMLGYDLYSSALADILSEPTMQPPICVGLYAQWGSGKSFLLKKLEDEMKTFAGQQIEPLFQFSWLIVFLTLLLCGGLGLLFAFTVHPNLGIAVSLSFLALLYIFFIVIYFGGRREGESWNWAWVLSTRLARHIGYLELLLKLMFVNPPELPEQTTKALPVRFLFTDYNRLSSVGGETSLAEMIATLSDACEREFGFLATRLFRVFKTEDTQGKKKWKKTCCLPSFVIFLFIIGCIISGITLLAIFRVDPKHLTVNAVLISIASVVGLAFVLNCRTWWQVLDSLLNSQRKRLHNAASKLHKLKSEGFMKVLKCEVELMARMAKTIDSFTQNQTRLVVIIDGLDACEQDKVLQMLDTVRVLFSKGPFIAIFASDPHIIIKAINQNLNSVLRDSNINGHDYMRNIVHLPVFLNSRGLSNARKFLVTSATNGDVPCSDTTGIQEDADRRVSQNSLGEMTKLGSKTALNRRDTYRRRQMQRTITRQMSFDLTKLLVTEDWFSDISPQTMRRLLNIVSVTGRLLRANQISFNWDRLASWINLTEQWPYRTSWLILYLEETEGIPDQMTLKTIYERISKNIPTTKDVEPLLEIDGDIRNFEVFLSSRTPVLVARDVKVFLPCTVNLDPKLREIIADVRAAREQISIGGLAYPPLPLHEGPPRAPSGYSQPPSVCSSTSFNGPFAGGVVSPQPHSSYYSGMTGPQHPFYNRPFFAPYLYTPRYYPGGSQHLISRPSVKTSLPRDQNNGLEVIKEDAAEGLSSPTDSSRGSGPAPGPVVLLNSLNVDAVCEKLKQIEGLDQSMLPQYCTTIKKANINGRVLAQCNIDELKKEMNMNFGDWHLFRSTVLEMRNAESHVVPEDPRFLSESSSGPAPHGEPARRASHNELPHTELSSQTPYTLNFSFEELNTLGLDEGAPRHSNLSWQSQTRRTPSLSSLNSQDSSIEISKLTDKVQAEYRDAYREYIAQMSQLEGGPGSTTISGRSSPHSTYYMGQSSSGGSIHSNLEQEKGKDSEPKPDDGRKSFLMKRGDVIDYSSSGVSTNDASPLDPITEEDEKSDQSGSKLLPGKKSSERSSLFQTDLKLKGSGLRYQKLPSDEDESGTEESDNTPLLKDDKDRKAEGKVERVPKSPEHSAEPIRTFIKAKEYLSDALLDKKDSSDSGVRSSESSPNHSLHNEVADDSQLEKANLIELEDDSHSGKRGIPHSLSGLQDPIIARMSICSEDKKSPSECSLIASSPEENWPACQKAYNLNRTPSTVTLNNNSAPANRANQNFDEMEGIRETSQVILRPSSSPNPTTIQNENLKSMTHKRSQRSSYTRLSKDPPELHAAASSESTGFGEERESIL
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9ULH0 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Phosphorylation | Uniprot | |
| Y12 | Phosphorylation | Uniprot | |
| K22 | Ubiquitination | Uniprot | |
| K27 | Ubiquitination | Uniprot | |
| K59 | Ubiquitination | Uniprot | |
| Y169 | Phosphorylation | Uniprot | |
| S203 | Phosphorylation | Uniprot | |
| Y215 | Phosphorylation | Uniprot | |
| T216 | Phosphorylation | Uniprot | |
| S244 | Phosphorylation | Uniprot | |
| K291 | Ubiquitination | Uniprot | |
| K303 | Ubiquitination | Uniprot | |
| Y307 | Phosphorylation | Uniprot | |
| K341 | Ubiquitination | Uniprot | |
| K367 | Ubiquitination | Uniprot | |
| Y398 | Phosphorylation | Uniprot | |
| T406 | Phosphorylation | Uniprot | |
| K416 | Ubiquitination | Uniprot | |
| Y465 | Phosphorylation | Uniprot | |
| K641 | Ubiquitination | Uniprot | |
| K732 | Ubiquitination | Uniprot | |
| K737 | Ubiquitination | Uniprot | |
| K743 | Acetylation | Uniprot | |
| K746 | Acetylation | Uniprot | |
| K746 | Ubiquitination | Uniprot | |
| Y833 | Phosphorylation | Uniprot | |
| K854 | Ubiquitination | Uniprot | |
| S882 | Phosphorylation | Uniprot | |
| S885 | Phosphorylation | Uniprot | |
| K891 | Ubiquitination | Uniprot | |
| S894 | Phosphorylation | Uniprot | |
| K895 | Ubiquitination | Uniprot | |
| T903 | Phosphorylation | Uniprot | |
| T912 | Phosphorylation | Uniprot | |
| T914 | Phosphorylation | Uniprot | |
| S918 | Phosphorylation | P98161 (PKD1) | Uniprot |
| T927 | Phosphorylation | Uniprot | |
| S932 | Phosphorylation | Uniprot | |
| S947 | Phosphorylation | Uniprot | |
| T949 | Phosphorylation | Uniprot | |
| K1007 | Ubiquitination | Uniprot | |
| K1013 | Ubiquitination | Uniprot | |
| K1057 | Ubiquitination | Uniprot | |
| Y1079 | Phosphorylation | Uniprot | |
| Y1096 | Phosphorylation | Uniprot | |
| Y1125 | Phosphorylation | Uniprot | |
| Y1144 | Phosphorylation | Uniprot | |
| Y1146 | Phosphorylation | Uniprot | |
| R1149 | Methylation | Uniprot | |
| Y1150 | Phosphorylation | Uniprot | |
| Y1151 | Phosphorylation | Uniprot | |
| S1155 | Phosphorylation | Uniprot | |
| S1163 | Phosphorylation | Uniprot | |
| K1165 | Ubiquitination | Uniprot | |
| S1167 | Phosphorylation | Uniprot | |
| S1294 | Phosphorylation | Uniprot | |
| S1295 | Phosphorylation | Uniprot | |
| S1296 | Phosphorylation | Uniprot | |
| S1309 | Phosphorylation | Uniprot | |
| Y1324 | Phosphorylation | Uniprot | |
| S1329 | Phosphorylation | Uniprot | |
| S1349 | Phosphorylation | Uniprot | |
| S1352 | Phosphorylation | Uniprot | |
| T1357 | Phosphorylation | Uniprot | |
| S1359 | Phosphorylation | Uniprot | |
| S1361 | Phosphorylation | Uniprot | |
| S1362 | Phosphorylation | Uniprot | |
| S1365 | Phosphorylation | Uniprot | |
| S1373 | Phosphorylation | Uniprot | |
| K1378 | Ubiquitination | Uniprot | |
| Y1383 | Phosphorylation | Uniprot | |
| Y1387 | Phosphorylation | Uniprot | |
| Y1390 | Phosphorylation | Uniprot | |
| S1410 | Phosphorylation | Uniprot | |
| S1411 | Phosphorylation | Uniprot | |
| Y1460 | Phosphorylation | Uniprot | |
| S1471 | Phosphorylation | Uniprot | |
| T1477 | Phosphorylation | Uniprot | |
| S1486 | Phosphorylation | Uniprot | |
| S1488 | Phosphorylation | Uniprot | |
| S1521 | Phosphorylation | Uniprot | |
| S1526 | Phosphorylation | Uniprot | |
| T1528 | Phosphorylation | Uniprot | |
| T1534 | Phosphorylation | Uniprot | |
| S1555 | Phosphorylation | Uniprot | |
| S1559 | Phosphorylation | Uniprot | |
| Y1572 | Phosphorylation | Uniprot | |
| S1574 | Phosphorylation | Uniprot | |
| K1580 | Ubiquitination | Uniprot | |
| S1590 | Phosphorylation | Uniprot | |
| S1594 | Phosphorylation | Uniprot | |
| S1598 | Phosphorylation | Uniprot | |
| S1607 | Phosphorylation | Uniprot | |
| S1621 | Phosphorylation | Uniprot | |
| S1623 | Phosphorylation | Uniprot | |
| K1625 | Ubiquitination | Uniprot | |
| S1631 | Phosphorylation | Uniprot | |
| S1633 | Phosphorylation | Uniprot | |
| S1661 | Phosphorylation | Uniprot | |
| S1662 | Phosphorylation | Uniprot | |
| Y1674 | Phosphorylation | Uniprot | |
| T1679 | Phosphorylation | Uniprot | |
| S1681 | Phosphorylation | Uniprot | |
| T1682 | Phosphorylation | Uniprot | |
| T1684 | Phosphorylation | Uniprot | |
| S1689 | Phosphorylation | Uniprot | |
| S1709 | Phosphorylation | Uniprot | |
| S1716 | Phosphorylation | Uniprot | |
| S1718 | Phosphorylation | Uniprot | |
| S1731 | Phosphorylation | Uniprot | |
| K1735 | Acetylation | Uniprot | |
| S1737 | Phosphorylation | Uniprot | |
| S1741 | Phosphorylation | Uniprot | |
| S1746 | Phosphorylation | Uniprot | |
| S1757 | Phosphorylation | Uniprot |
研究背景
Promotes a prolonged MAP-kinase signaling by neurotrophins through activation of a Rap1-dependent mechanism. Provides a docking site for the CRKL-C3G complex, resulting in Rap1-dependent sustained ERK activation. May play an important role in regulating postsynaptic signal transduction through the syntrophin-mediated localization of receptor tyrosine kinases such as EPHA4. In cooperation with SNTA1 can enhance EPHA4-induced JAK/STAT activation. Plays a role in nerve growth factor (NGF)-induced recruitment of RAPGEF2 to late endosomes and neurite outgrowth. May play a role in neurotrophin- and ephrin-mediated neuronal outgrowth and in axon guidance during neural development and in neuronal regeneration (By similarity). Modulates stress-induced apoptosis of melanoma cells via regulation of the MEK/ERK signaling pathway.
Tyrosine phosphorylated by NTRK1, NTRK2, EPHB2 and EPHA4. Phosphorylation at Ser-918 is induced by phorbol ester treatment. Phosphorylation by NTRK2 is induced by brain-derived neurotrophic factor (BDNF) and neurotrophin-4/5. Phosphorylation by NTRK1 is induced by nerve growth factor (NGF) (By similarity).
Membrane>Multi-pass membrane protein. Late endosome.
Note: Localized at late endosome before or after nerve growth factor (NGF) stimulation.
Abundant in developing and adult neural tissues as well as neuroendocrine cells and dendritic cells. Overexpressed in melanoma and melanoma cell lines.
Found in a complex, at least composed of KIDINS220, MAGI2, NTRK1 and RAPGEF2; the complex is mainly formed at late endosomes in a nerve growth factor (NGF)-dependent manner. Interacts with RAPGEF2; the interaction is strengthened after NGF stimulation. Isoform 2 interacts (via C-terminal domain) with MAGI2 isoform 1 (via PDZ domain). Interacts with NTRK1, NTRK2, NTRK3, ERKL and NGFR. Can form a ternary complex with NGFR and NTRK1 and this complex is affected by the expression levels of KIDINS220/ARMS. An increase in KIDINS220/ARMS expression leads to a decreased association of NGFR and NTRK1. Interacts (via PDZ-binding motif) with SNTA1 and SNTB2 (via PDZ domains). Interacts with EPHA4 and PRKD1.
The transmembrane domain mediates interaction with NTRK1.
研究领域
· Organismal Systems > Nervous system > Neurotrophin signaling pathway. (View pathway)
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