产品: | NAT10 抗体 |
货号: | DF12427 |
描述: | Rabbit polyclonal antibody to NAT10 |
应用: | WB IHC |
反应: | Human, Mouse, Rat |
预测: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
分子量: | 116 kDa; 116kD(Calculated). |
蛋白号: | Q9H0A0 |
RRID: | AB_2845232 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF12427, RRID:AB_2845232.
展开/折叠
ALP; DKFZp434C116; FLJ10774; FLJ12179; FLJ23850; hALP; KIAA1709; N acetyltransferase 10; N acetyltransferase 10 GCN5 related; N acetyltransferase like; N acetyltransferase like protein; N-acetyltransferase 10; NAT10; NAT10_HUMAN; NET43;
抗原和靶标
- Q9H0A0 NAT10_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MHRKKVDNRIRILIENGVAERQRSLFVVVGDRGKDQVVILHHMLSKATVKARPSVLWCYKKELGFSSHRKKRMRQLQKKIKNGTLNIKQDDPFELFIAATNIRYCYYNETHKILGNTFGMCVLQDFEALTPNLLARTVETVEGGGLVVILLRTMNSLKQLYTVTMDVHSRYRTEAHQDVVGRFNERFILSLASCKKCLVIDDQLNILPISSHVATMEALPPQTPDESLGPSDLELRELKESLQDTQPVGVLVDCCKTLDQAKAVLKFIEGISEKTLRSTVALTAARGRGKSAALGLAIAGAVAFGYSNIFVTSPSPDNLHTLFEFVFKGFDALQYQEHLDYEIIQSLNPEFNKAVIRVNVFREHRQTIQYIHPADAVKLGQAELVVIDEAAAIPLPLVKSLLGPYLVFMASTINGYEGTGRSLSLKLIQQLRQQSAQSQVSTTAENKTTTTARLASARTLYEVSLQESIRYAPGDAVEKWLNDLLCLDCLNITRIVSGCPLPEACELYYVNRDTLFCYHKASEVFLQRLMALYVASHYKNSPNDLQMLSDAPAHHLFCLLPPVPPTQNALPEVLAVIQVCLEGEISRQSILNSLSRGKKASGDLIPWTVSEQFQDPDFGGLSGGRVVRIAVHPDYQGMGYGSRALQLLQMYYEGRFPCLEEKVLETPQEIHTVSSEAVSLLEEVITPRKDLPPLLLKLNERPAERLDYLGVSYGLTPRLLKFWKRAGFVPVYLRQTPNDLTGEHSCIMLKTLTDEDEADQGGWLAAFWKDFRRRFLALLSYQFSTFSPSLALNIIQNRNMGKPAQPALSREELEALFLPYDLKRLEMYSRNMVDYHLIMDMIPAISRIYFLNQLGDLALSAAQSALLLGIGLQHKSVDQLEKEIELPSGQLMGLFNRIIRKVVKLFNEVQEKAIEEQMVAAKDVVMEPTMKTLSDDLDEAAKEFQEKHKKEVGKLKSMDLSEYIIRGDDEEWNEVLNKAGPNASIISLKSDKKRKLEAKQEPKQSKKLKNRETKNKKDMKLKRKK
种属预测
score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9H0A0 作为底物
Site | PTM Type | Enzyme | Source |
---|---|---|---|
R32 | Methylation | Uniprot | |
K60 | Acetylation | Uniprot | |
K61 | Acetylation | Uniprot | |
K61 | Ubiquitination | Uniprot | |
S67 | Phosphorylation | Uniprot | |
T137 | Phosphorylation | Uniprot | |
S156 | Phosphorylation | Uniprot | |
T164 | Phosphorylation | Uniprot | |
T173 | Phosphorylation | Uniprot | |
K195 | Methylation | Uniprot | |
T223 | Phosphorylation | Uniprot | |
K239 | Ubiquitination | Uniprot | |
K256 | Ubiquitination | Uniprot | |
K262 | Ubiquitination | Uniprot | |
K266 | Ubiquitination | Uniprot | |
S272 | Phosphorylation | Uniprot | |
K274 | Ubiquitination | Uniprot | |
Y416 | Phosphorylation | Uniprot | |
K426 | Acetylation | Uniprot | |
K426 | Ubiquitination | Uniprot | |
S435 | Phosphorylation | Uniprot | |
S438 | Phosphorylation | Uniprot | |
K447 | Ubiquitination | Uniprot | |
K520 | Ubiquitination | Uniprot | |
S589 | Phosphorylation | Uniprot | |
S593 | Phosphorylation | Uniprot | |
K599 | Ubiquitination | Uniprot | |
Y635 | Phosphorylation | Uniprot | |
T666 | Phosphorylation | Uniprot | |
S679 | Phosphorylation | Uniprot | |
T686 | Phosphorylation | Uniprot | |
K689 | Ubiquitination | Uniprot | |
T716 | Phosphorylation | Uniprot | |
T741 | Phosphorylation | Uniprot | |
S809 | Phosphorylation | Uniprot | |
Y820 | Phosphorylation | Uniprot | |
K823 | Acetylation | Uniprot | |
K823 | Ubiquitination | Uniprot | |
K904 | Acetylation | Uniprot | |
K904 | Ubiquitination | Uniprot | |
T929 | Phosphorylation | Uniprot | |
T932 | Phosphorylation | Uniprot | |
S934 | Phosphorylation | Uniprot | |
K942 | Acetylation | Uniprot | |
K942 | Ubiquitination | Uniprot | |
K947 | Acetylation | Uniprot | |
K956 | Ubiquitination | Uniprot | |
S957 | Phosphorylation | Uniprot | |
Y963 | Phosphorylation | Uniprot | |
K978 | Ubiquitination | Uniprot | |
S984 | Phosphorylation | Uniprot | |
S987 | Phosphorylation | Uniprot | |
S990 | Phosphorylation | Uniprot |
研究背景
RNA cytidine acetyltransferase that catalyzes the formation of N(4)-acetylcytidine (ac4C) modification on mRNAs, 18S rRNA and tRNAs. Catalyzes ac4C modification of a broad range of mRNAs, enhancing mRNA stability and translation. mRNA ac4C modification is frequently present within wobble cytidine sites and promotes translation efficiency. Mediates the formation of ac4C at position 1842 in 18S rRNA. May also catalyze the formation of ac4C at position 1337 in 18S rRNA (By similarity). Required for early nucleolar cleavages of precursor rRNA at sites A0, A1 and A2 during 18S rRNA synthesis. Catalyzes the formation of ac4C in serine and leucine tRNAs (By similarity). Requires the tRNA-binding adapter protein THUMPD1 for full tRNA acetyltransferase activity but not for 18S rRNA acetylation. In addition to RNA acetyltransferase activity, also able to acetylate lysine residues of proteins, such as histones, microtubules, p53/TP53 and MDM2, in vitro. The relevance of the protein lysine acetyltransferase activity is however unsure in vivo. Activates telomerase activity by stimulating the transcription of TERT, and may also regulate telomerase function by affecting the balance of telomerase subunit assembly, disassembly, and localization.
Acetylation at Lys-426 is required to activation of rRNA transcription. May be autoacetylated; however ability to autoacetylate in vivo requires additional evidences.
Nucleus>Nucleolus. Midbody.
Note: Nucleolar in interphase and redistributes to the perichromosomal layer and to the midbody during telophase.
Interacts with THUMPD1. Interacts with SUN1 (via N-terminus). Interacts with TERT.
Belongs to the RNA cytidine acetyltransferase family. NAT10 subfamily.
研究领域
· Genetic Information Processing > Translation > Ribosome biogenesis in eukaryotes.
文献引用
Application: IHC Species: Human Sample:
Application: WB Species: Human Sample:
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