| 产品: | p107 抗体 |
| 货号: | DF12438 |
| 描述: | Rabbit polyclonal antibody to p107 |
| 应用: | WB IHC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus |
| 分子量: | 119 kDa; 121kD(Calculated). |
| 蛋白号: | P28749 |
| RRID: | AB_2845243 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF12438, RRID:AB_2845243.
展开/折叠
107 kDa retinoblastoma-associated protein; Cellular protein 107; Cellular protein p107; CP107; MGC40006; p107; PRB1; RBL1; RBL1_HUMAN; Retinoblastoma like protein 1; retinoblastoma-like 1 (p107); Retinoblastoma-like protein 1;
抗原和靶标
- P28749 RBL1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MFEDKPHAEGAAVVAAAGEALQALCQELNLDEGSAAEALDDFTAIRGNYSLEGEVTHWLACSLYVACRKSIIPTVGKGIMEGNCVSLTRILRSAKLSLIQFFSKMKKWMDMSNLPQEFRERIERLERNFEVSTVIFKKYEPIFLDIFQNPYEEPPKLPRSRKQRRIPCSVKDLFNFCWTLFVYTKGNFRMIGDDLVNSYHLLLCCLDLIFANAIMCPNRQDLLNPSFKGLPSDFHTADFTASEEPPCIIAVLCELHDGLLVEAKGIKEHYFKPYISKLFDRKILKGECLLDLSSFTDNSKAVNKEYEEYVLTVGDFDERIFLGADAEEEIGTPRKFTRDTPLGKLTAQANVEYNLQQHFEKKRSFAPSTPLTGRRYLREKEAVITPVASATQSVSRLQSIVAGLKNAPSDQLINIFESCVRNPVENIMKILKGIGETFCQHYTQSTDEQPGSHIDFAVNRLKLAEILYYKILETVMVQETRRLHGMDMSVLLEQDIFHRSLMACCLEIVLFAYSSPRTFPWIIEVLNLQPFYFYKVIEVVIRSEEGLSRDMVKHLNSIEEQILESLAWSHDSALWEALQVSANKVPTCEEVIFPNNFETGNGGNVQGHLPLMPMSPLMHPRVKEVRTDSGSLRRDMQPLSPISVHERYSSPTAGSAKRRLFGEDPPKEMLMDKIITEGTKLKIAPSSSITAENVSILPGQTLLTMATAPVTGTTGHKVTIPLHGVANDAGEITLIPLSMNTNQESKVKSPVSLTAHSLIGASPKQTNLTKAQEVHSTGINRPKRTGSLALFYRKVYHLASVRLRDLCLKLDVSNELRRKIWTCFEFTLVHCPDLMKDRHLDQLLLCAFYIMAKVTKEERTFQEIMKSYRNQPQANSHVYRSVLLKSIPREVVAYNKNINDDFEMIDCDLEDATKTPDCSSGPVKEERGDLIKFYNTIYVGRVKSFALKYDLANQDHMMDAPPLSPFPHIKQQPGSPRRISQQHSIYISPHKNGSGLTPRSALLYKFNGSPSKSLKDINNMIRQGEQRTKKRVIAIDSDAESPAKRVCQENDDVLLKRLQDVVSERANH
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P28749 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T88 | Phosphorylation | Uniprot | |
| S93 | Phosphorylation | Uniprot | |
| S103 | Phosphorylation | Uniprot | |
| K300 | Ubiquitination | Uniprot | |
| K304 | Ubiquitination | Uniprot | |
| T332 | Phosphorylation | Uniprot | |
| T337 | Phosphorylation | Uniprot | |
| T340 | Phosphorylation | Uniprot | |
| S368 | Phosphorylation | Uniprot | |
| T369 | Phosphorylation | P11802 (CDK4) | Uniprot |
| T372 | Phosphorylation | Uniprot | |
| T385 | Phosphorylation | Uniprot | |
| S389 | Phosphorylation | Uniprot | |
| Y468 | Phosphorylation | Uniprot | |
| Y469 | Phosphorylation | Uniprot | |
| S615 | Phosphorylation | Uniprot | |
| S640 | Phosphorylation | P11802 (CDK4) , P24941 (CDK2) | Uniprot |
| S643 | Phosphorylation | Uniprot | |
| Y648 | Phosphorylation | Uniprot | |
| S650 | Phosphorylation | P24941 (CDK2) , P11802 (CDK4) | Uniprot |
| T652 | Phosphorylation | Uniprot | |
| K657 | Ubiquitination | Uniprot | |
| S749 | Phosphorylation | Uniprot | |
| S757 | Phosphorylation | Uniprot | |
| S762 | Phosphorylation | Uniprot | |
| T769 | Phosphorylation | Uniprot | |
| Y792 | Phosphorylation | Uniprot | |
| T915 | Phosphorylation | Uniprot | |
| S919 | Phosphorylation | Uniprot | |
| Y938 | Phosphorylation | Uniprot | |
| Y949 | Phosphorylation | Uniprot | |
| S964 | Phosphorylation | P11802 (CDK4) | Uniprot |
| S975 | Phosphorylation | P11802 (CDK4) | Uniprot |
| S980 | Phosphorylation | Uniprot | |
| S984 | Phosphorylation | Uniprot | |
| Y986 | Phosphorylation | Uniprot | |
| S988 | Phosphorylation | Uniprot | |
| S994 | Phosphorylation | Uniprot | |
| T997 | Phosphorylation | Uniprot | |
| S1000 | Phosphorylation | Uniprot | |
| Y1004 | Phosphorylation | Uniprot | |
| S1009 | Phosphorylation | Uniprot | |
| S1011 | Phosphorylation | Uniprot | |
| S1037 | Phosphorylation | Uniprot | |
| S1041 | Phosphorylation | Uniprot |
研究背景
Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation (By similarity). Recruits and targets histone methyltransferases KMT5B and KMT5C, leading to epigenetic transcriptional repression (By similarity). Controls histone H4 'Lys-20' trimethylation (By similarity). Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters (By similarity). Potent inhibitor of E2F-mediated trans-activation. May act as a tumor suppressor.
Cell-cycle arrest properties are inactivated by phosphorylation on Thr-332, Ser-640, Ser-964 and Ser-975 by CDK4.
Nucleus.
Component of the DREAM complex (also named LINC complex) at least composed of E2F4, E2F5, LIN9, LIN37, LIN52, LIN54, MYBL1, MYBL2, RBL1, RBL2, RBBP4, TFDP1 and TFDP2. The complex exists in quiescent cells where it represses cell cycle-dependent genes. It dissociates in S phase when LIN9, LIN37, LIN52 and LIN54 form a subcomplex that binds to MYBL2. Interacts with AATF. Interacts with KDM5A. Interacts with KMT5B and KMT5C (By similarity). Interacts with USP4 (By similarity). Interacts with RBBP9 (By similarity).
(Microbial infection) Interacts with SV40 and JC virus large T antigens. Large T antigen, but not E1A, binds only to the unphosphorylated form.
Belongs to the retinoblastoma protein (RB) family.
研究领域
· Cellular Processes > Cell growth and death > Cell cycle. (View pathway)
· Cellular Processes > Cell growth and death > Cellular senescence. (View pathway)
· Environmental Information Processing > Signal transduction > TGF-beta signaling pathway. (View pathway)
· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.
· Human Diseases > Cancers: Overview > Viral carcinogenesis.
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